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Evaluation of the Antimicrobial Effect of Chitosan/Polyvinyl Alcohol Electrospun Nanofibers Containing Mafenide Acetate.

Abbaspour M, Sharif Makhmalzadeh B, Rezaee B, Shoja S, Ahangari Z - Jundishapur J Microbiol (2015)

Bottom Line: The results indicated that all nanofibrous films, with and without drug, can prevent bacterial penetration.Incorporation of mafenide acetate into chitosan/PVA nanofibers enhanced their antimicrobial activity against P. aeruginosa and S. aureus.Overall, the results showed that chitosan/polyvinyl alcohol (PVA) nanofibrous films are applicable for use as a wound dressing with protective, healing, and antimicrobial effects.

View Article: PubMed Central - PubMed

Affiliation: Nanotechnology Research Center, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.

ABSTRACT

Background: Chitosan, an important biodegradable and biocompatible polymer, has demonstrated wound-healing and antimicrobial properties.

Objectives: This study aimed to evaluate the antimicrobial properties of mafenide acetate-loaded nanofibrous films, prepared by the electrospinning technique, using chitosan and polyvinyl alcohol (PVA).

Materials and methods: A 3(2) full factorial design was used for formulating electrospinning solutions. The chitosan percentage in chitosan/PVA solutions (0%, 10%, and 30%) and the drug content (0%, 20%, and 40%) were chosen as independent variables. The release rate of mafenide acetate from nanofibrous films and their microbial penetration were evaluated. The antimicrobial activity of different nanofibrous film formulations against Staphylococcus aureus and Pseudomonas aeruginosa was studied.

Results: The results indicated that all nanofibrous films, with and without drug, can prevent bacterial penetration. Incorporation of mafenide acetate into chitosan/PVA nanofibers enhanced their antimicrobial activity against P. aeruginosa and S. aureus.

Conclusions: Overall, the results showed that chitosan/polyvinyl alcohol (PVA) nanofibrous films are applicable for use as a wound dressing with protective, healing, and antimicrobial effects.

No MeSH data available.


Related in: MedlinePlus

Scanning Electron Micrographs of PVA: Chitosan Nanofibers (PC) Containing Mafenide Acetate; A) PC 90:10 + 20% drug, B) PC 90:10 + 40% Drug, C) PC 70:30 + 20% Drug, D) PC 70:30 + 40% Drug, (Magnification: 4000 ×, Scale bar: 2 µm)
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fig23276: Scanning Electron Micrographs of PVA: Chitosan Nanofibers (PC) Containing Mafenide Acetate; A) PC 90:10 + 20% drug, B) PC 90:10 + 40% Drug, C) PC 70:30 + 20% Drug, D) PC 70:30 + 40% Drug, (Magnification: 4000 ×, Scale bar: 2 µm)

Mentions: Morphological evaluation of the film formulations showed that pure PVA generated smooth and uniform nanofibers with a mean diameter of 1064 nm, which upon addition of 20% and 40% mafenide acetate, decreased in diameter to 418 and 102 nm, respectively (Figure 1).


Evaluation of the Antimicrobial Effect of Chitosan/Polyvinyl Alcohol Electrospun Nanofibers Containing Mafenide Acetate.

Abbaspour M, Sharif Makhmalzadeh B, Rezaee B, Shoja S, Ahangari Z - Jundishapur J Microbiol (2015)

Scanning Electron Micrographs of PVA: Chitosan Nanofibers (PC) Containing Mafenide Acetate; A) PC 90:10 + 20% drug, B) PC 90:10 + 40% Drug, C) PC 70:30 + 20% Drug, D) PC 70:30 + 40% Drug, (Magnification: 4000 ×, Scale bar: 2 µm)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4644308&req=5

fig23276: Scanning Electron Micrographs of PVA: Chitosan Nanofibers (PC) Containing Mafenide Acetate; A) PC 90:10 + 20% drug, B) PC 90:10 + 40% Drug, C) PC 70:30 + 20% Drug, D) PC 70:30 + 40% Drug, (Magnification: 4000 ×, Scale bar: 2 µm)
Mentions: Morphological evaluation of the film formulations showed that pure PVA generated smooth and uniform nanofibers with a mean diameter of 1064 nm, which upon addition of 20% and 40% mafenide acetate, decreased in diameter to 418 and 102 nm, respectively (Figure 1).

Bottom Line: The results indicated that all nanofibrous films, with and without drug, can prevent bacterial penetration.Incorporation of mafenide acetate into chitosan/PVA nanofibers enhanced their antimicrobial activity against P. aeruginosa and S. aureus.Overall, the results showed that chitosan/polyvinyl alcohol (PVA) nanofibrous films are applicable for use as a wound dressing with protective, healing, and antimicrobial effects.

View Article: PubMed Central - PubMed

Affiliation: Nanotechnology Research Center, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.

ABSTRACT

Background: Chitosan, an important biodegradable and biocompatible polymer, has demonstrated wound-healing and antimicrobial properties.

Objectives: This study aimed to evaluate the antimicrobial properties of mafenide acetate-loaded nanofibrous films, prepared by the electrospinning technique, using chitosan and polyvinyl alcohol (PVA).

Materials and methods: A 3(2) full factorial design was used for formulating electrospinning solutions. The chitosan percentage in chitosan/PVA solutions (0%, 10%, and 30%) and the drug content (0%, 20%, and 40%) were chosen as independent variables. The release rate of mafenide acetate from nanofibrous films and their microbial penetration were evaluated. The antimicrobial activity of different nanofibrous film formulations against Staphylococcus aureus and Pseudomonas aeruginosa was studied.

Results: The results indicated that all nanofibrous films, with and without drug, can prevent bacterial penetration. Incorporation of mafenide acetate into chitosan/PVA nanofibers enhanced their antimicrobial activity against P. aeruginosa and S. aureus.

Conclusions: Overall, the results showed that chitosan/polyvinyl alcohol (PVA) nanofibrous films are applicable for use as a wound dressing with protective, healing, and antimicrobial effects.

No MeSH data available.


Related in: MedlinePlus