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Antibiotic Resistance and Biofilm Formation of Enterococcus faecalis in Patient and Environmental Samples.

Talebi M, Asghari Moghadam N, Mamooii Z, Enayati M, Saifi M, Pourshafie MR - Jundishapur J Microbiol (2015)

Bottom Line: The results indicated that, 86% of patient and 29% of environmental isolates carried ebp genes.The RFLP of the ebp showed no genetic variations amongst the isolates.The results of the antibiotic resistance and other data suggest that there is a possible common clone of E. faecalis, which could rapidly disseminate in patients and the environment.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, IR Iran.

ABSTRACT

Background: Enterococci are opportunistic pathogens and are a major factor in nosocomial infections. They may contain ebp operon, which upon expression makes them highly prone to biofilm formation on biotic and abiotic surfaces.

Objectives: The aim of the current study was to detect the polymorphism of ebp genes in Enterococcus faecalis.

Materials and methods: Samples were isolated from patients (n = 58) and hospital environments (n = 32) of two hospitals in Tehran, Iran. All enterococcal species were identified by species-specific polymerase chain reaction (PCR); the antibiotic resistance pattern against nine antibiotics was determined. The ebp A, ebp B, ebp C and srt C genes were detected by PCR and the biofilm formation by the isolates was evaluated using the microtiter plate method. The genetic diversity of ebp genes was analyzed by restriction fragment length polymorphism (RFLP).

Results: The results indicated that, 86% of patient and 29% of environmental isolates carried ebp genes. The ability of the isolates to strongly attach was 62% and 71% for patient and environmental samples, respectively. The RFLP of the ebp showed no genetic variations amongst the isolates.

Conclusions: The results of the antibiotic resistance and other data suggest that there is a possible common clone of E. faecalis, which could rapidly disseminate in patients and the environment.

No MeSH data available.


Related in: MedlinePlus

Antibiotic Resistance Pattern Amongst Patient and Environmental Isolates
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fig23370: Antibiotic Resistance Pattern Amongst Patient and Environmental Isolates

Mentions: Among the clinical isolates, 86% of isolates were resistant to tetracycline (86%). The resistance observed against teicoplanin (3%), vancomycin (9%), linezolid (9%) and ampicillin (9%) was low. Among environmental isolates only 25% were sensitive to tetracycline with no resistance against vancomycin, teicoplanin, linezolid and ampicillin. Overall, 20 different antibiotic resistance patterns were obtained for 58 patient isolates and nine for environmental isolates (Figure 1).


Antibiotic Resistance and Biofilm Formation of Enterococcus faecalis in Patient and Environmental Samples.

Talebi M, Asghari Moghadam N, Mamooii Z, Enayati M, Saifi M, Pourshafie MR - Jundishapur J Microbiol (2015)

Antibiotic Resistance Pattern Amongst Patient and Environmental Isolates
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4644270&req=5

fig23370: Antibiotic Resistance Pattern Amongst Patient and Environmental Isolates
Mentions: Among the clinical isolates, 86% of isolates were resistant to tetracycline (86%). The resistance observed against teicoplanin (3%), vancomycin (9%), linezolid (9%) and ampicillin (9%) was low. Among environmental isolates only 25% were sensitive to tetracycline with no resistance against vancomycin, teicoplanin, linezolid and ampicillin. Overall, 20 different antibiotic resistance patterns were obtained for 58 patient isolates and nine for environmental isolates (Figure 1).

Bottom Line: The results indicated that, 86% of patient and 29% of environmental isolates carried ebp genes.The RFLP of the ebp showed no genetic variations amongst the isolates.The results of the antibiotic resistance and other data suggest that there is a possible common clone of E. faecalis, which could rapidly disseminate in patients and the environment.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, IR Iran.

ABSTRACT

Background: Enterococci are opportunistic pathogens and are a major factor in nosocomial infections. They may contain ebp operon, which upon expression makes them highly prone to biofilm formation on biotic and abiotic surfaces.

Objectives: The aim of the current study was to detect the polymorphism of ebp genes in Enterococcus faecalis.

Materials and methods: Samples were isolated from patients (n = 58) and hospital environments (n = 32) of two hospitals in Tehran, Iran. All enterococcal species were identified by species-specific polymerase chain reaction (PCR); the antibiotic resistance pattern against nine antibiotics was determined. The ebp A, ebp B, ebp C and srt C genes were detected by PCR and the biofilm formation by the isolates was evaluated using the microtiter plate method. The genetic diversity of ebp genes was analyzed by restriction fragment length polymorphism (RFLP).

Results: The results indicated that, 86% of patient and 29% of environmental isolates carried ebp genes. The ability of the isolates to strongly attach was 62% and 71% for patient and environmental samples, respectively. The RFLP of the ebp showed no genetic variations amongst the isolates.

Conclusions: The results of the antibiotic resistance and other data suggest that there is a possible common clone of E. faecalis, which could rapidly disseminate in patients and the environment.

No MeSH data available.


Related in: MedlinePlus