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Noncontiguous finished genome sequence and description of Weeksella massiliensis sp. nov.

Sankar SA, Lo CI, Fall B, Sambe-Ba B, Mediannikov O, Diallo I, Labas N, Faye N, Wade B, Raoult D, Fournier PE, Fenollar F - New Microbes New Infect (2015)

Bottom Line: The sequenced genome is 2 562 781 bp with one chromosome but no plasmid.It exhibits a G + C content of 35.9% and contains 2390 protein-coding and 56 RNA genes, including a complete rRNA operon.On the basis of these data, we propose the creation of Weeksella massiliensis sp. nov.

View Article: PubMed Central - PubMed

Affiliation: )Aix-Marseille Université, URMITE, UM63, CNRS 7278, IRD 198, Inserm U1095, Faculté de médecine, Marseille, France.

ABSTRACT
Strain FF8(T) (= CSUR P860 = DSM 28259) was isolated in Dakar, Senegal, from the urine of a 65-year-old man with acute cystitis. This strain shows a similarity of sequence of 16S rRNA of 98.38% with Weeksella virosa, and its GenBank accession numbers are HG931340 and CCMH00000000. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis had a poor score, ranging from 1.32 to 1.56, that did not allow identification of the bacterium. Using a polyphasic study made of phenotypic and genomic analyses, strain FF8(T) was a Gram-negative, aerobic rod and a member of the family Flavobacteriaceae. The sequenced genome is 2 562 781 bp with one chromosome but no plasmid. It exhibits a G + C content of 35.9% and contains 2390 protein-coding and 56 RNA genes, including a complete rRNA operon. On the basis of these data, we propose the creation of Weeksella massiliensis sp. nov.

No MeSH data available.


Related in: MedlinePlus

Graphical circular map of Weeksella massiliensis strain FF8T chromosome. From outside in, outer two circles show open reading frames oriented in forward (colored by COGs categories) and reverse (colored by COGs categories) directions, respectively. Third circle marks rRNA gene operon (red) and tRNA genes (green). Fourth circle shows G + C content plot. Innermost circle shows GC skew, with purple indicating negative values and olive positive values. COGs, Clusters of Orthologous Groups (COGs) database.
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fig6: Graphical circular map of Weeksella massiliensis strain FF8T chromosome. From outside in, outer two circles show open reading frames oriented in forward (colored by COGs categories) and reverse (colored by COGs categories) directions, respectively. Third circle marks rRNA gene operon (red) and tRNA genes (green). Fourth circle shows G + C content plot. Innermost circle shows GC skew, with purple indicating negative values and olive positive values. COGs, Clusters of Orthologous Groups (COGs) database.

Mentions: The genome of W. massiliensis strain FF8T is 2 562 781 bp long with a 35.9% G + C content (Fig. 6). Of the 2446 predicted genes, 2390 were protein-coding genes and 56 were RNAs genes. Three rRNA genes (one 16S rRNA, one 23S rRNA and one 5S rRNA) and 53 predicted tRNA genes were identified in the genome. A total of 1428 genes (58.38%) were assigned a putative function. Eighty genes were identified as ORFans (3.27%). The remaining genes were annotated as hypothetical proteins. The properties and the statistics of the genome are summarized in Table 5. The distribution of genes into COGs functional categories is presented in Table 6.


Noncontiguous finished genome sequence and description of Weeksella massiliensis sp. nov.

Sankar SA, Lo CI, Fall B, Sambe-Ba B, Mediannikov O, Diallo I, Labas N, Faye N, Wade B, Raoult D, Fournier PE, Fenollar F - New Microbes New Infect (2015)

Graphical circular map of Weeksella massiliensis strain FF8T chromosome. From outside in, outer two circles show open reading frames oriented in forward (colored by COGs categories) and reverse (colored by COGs categories) directions, respectively. Third circle marks rRNA gene operon (red) and tRNA genes (green). Fourth circle shows G + C content plot. Innermost circle shows GC skew, with purple indicating negative values and olive positive values. COGs, Clusters of Orthologous Groups (COGs) database.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4644262&req=5

fig6: Graphical circular map of Weeksella massiliensis strain FF8T chromosome. From outside in, outer two circles show open reading frames oriented in forward (colored by COGs categories) and reverse (colored by COGs categories) directions, respectively. Third circle marks rRNA gene operon (red) and tRNA genes (green). Fourth circle shows G + C content plot. Innermost circle shows GC skew, with purple indicating negative values and olive positive values. COGs, Clusters of Orthologous Groups (COGs) database.
Mentions: The genome of W. massiliensis strain FF8T is 2 562 781 bp long with a 35.9% G + C content (Fig. 6). Of the 2446 predicted genes, 2390 were protein-coding genes and 56 were RNAs genes. Three rRNA genes (one 16S rRNA, one 23S rRNA and one 5S rRNA) and 53 predicted tRNA genes were identified in the genome. A total of 1428 genes (58.38%) were assigned a putative function. Eighty genes were identified as ORFans (3.27%). The remaining genes were annotated as hypothetical proteins. The properties and the statistics of the genome are summarized in Table 5. The distribution of genes into COGs functional categories is presented in Table 6.

Bottom Line: The sequenced genome is 2 562 781 bp with one chromosome but no plasmid.It exhibits a G + C content of 35.9% and contains 2390 protein-coding and 56 RNA genes, including a complete rRNA operon.On the basis of these data, we propose the creation of Weeksella massiliensis sp. nov.

View Article: PubMed Central - PubMed

Affiliation: )Aix-Marseille Université, URMITE, UM63, CNRS 7278, IRD 198, Inserm U1095, Faculté de médecine, Marseille, France.

ABSTRACT
Strain FF8(T) (= CSUR P860 = DSM 28259) was isolated in Dakar, Senegal, from the urine of a 65-year-old man with acute cystitis. This strain shows a similarity of sequence of 16S rRNA of 98.38% with Weeksella virosa, and its GenBank accession numbers are HG931340 and CCMH00000000. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis had a poor score, ranging from 1.32 to 1.56, that did not allow identification of the bacterium. Using a polyphasic study made of phenotypic and genomic analyses, strain FF8(T) was a Gram-negative, aerobic rod and a member of the family Flavobacteriaceae. The sequenced genome is 2 562 781 bp with one chromosome but no plasmid. It exhibits a G + C content of 35.9% and contains 2390 protein-coding and 56 RNA genes, including a complete rRNA operon. On the basis of these data, we propose the creation of Weeksella massiliensis sp. nov.

No MeSH data available.


Related in: MedlinePlus