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Noncontiguous finished genome sequence and description of Virgibacillus massiliensis sp. nov., a moderately halophilic bacterium isolated from human gut.

Khelaifia S, Croce O, Lagier JC, Robert C, Couderc C, Di Pinto F, Davoust B, Djossou F, Raoult D, Fournier PE - New Microbes New Infect (2015)

Bottom Line: Here we describe its phenotypic characteristics and complete genome sequence.The 4 353 177 bp long genome exhibits a G + C content of 36.87% and contains 4394 protein-coding and 125 predicted RNA genes.Phylogenetically and genetically, strain Vm-c is a member of the genus Virgibacillus but is distinct enough to be classified as a new species.

View Article: PubMed Central - PubMed

Affiliation: Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UM 63, CNRS 7278, IRD 198, Inserm 1095, Institut Hospitalo-Universitaire Méditerranée-Infection, Faculté de médecine, Aix-Marseille Université, Marseille, France.

ABSTRACT
Strain Vm-5(T) was isolated from the stool specimen of a 10-year-old Amazonian boy. This bacterium is a Gram-positive, strictly aerobic rod, motile by a polar flagellum. Here we describe its phenotypic characteristics and complete genome sequence. The 4 353 177 bp long genome exhibits a G + C content of 36.87% and contains 4394 protein-coding and 125 predicted RNA genes. Phylogenetically and genetically, strain Vm-c is a member of the genus Virgibacillus but is distinct enough to be classified as a new species. We propose the creation of V. massiliensis sp. nov., whose type strain is strain Vm-5(T) (CSUR P971 = DSM 28587).

No MeSH data available.


Related in: MedlinePlus

Transmission electron microscopy of Virgibacillus massiliensis strain Vm-5T, using Morgagni 268D transmission electron microscope (Philips/FEI, Hillsboro, OR, USA) at operating voltage of 60 kV. Scale bar = 500 nm. Arrow indicates terminal spore.
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fig3: Transmission electron microscopy of Virgibacillus massiliensis strain Vm-5T, using Morgagni 268D transmission electron microscope (Philips/FEI, Hillsboro, OR, USA) at operating voltage of 60 kV. Scale bar = 500 nm. Arrow indicates terminal spore.

Mentions: Strain Vm-5 T was mesophilic and grew at temperatures ranging from 15 to 45°C, at an optimum temperature of 37°C. The isolate required NaCl for growth and grew at salinity ranging from 5 to 200 g/L of NaCl (optimum at 50 g/L). The optimal pH for growth was 7.5 (pH range 5 to 9). The growth of strain Vm-5T was tested under aerobic atmosphere, in the presence of 5% CO2 and in anaerobic and microaerophilic atmospheres created using GENbag anaer and GENbag microaer (bioMérieux, Marcy l’Etoile, France), respectively. The strain was strictly aerobic and grew in the presence of 5% CO2 but did not grow in microaerophilic or anaerobic atmosphere. The size (2 to 6 μm in length and 0.5 μm in diameter) and ultrastructure of cells were determined by negative staining transmission electron microscopy (Fig. 3).


Noncontiguous finished genome sequence and description of Virgibacillus massiliensis sp. nov., a moderately halophilic bacterium isolated from human gut.

Khelaifia S, Croce O, Lagier JC, Robert C, Couderc C, Di Pinto F, Davoust B, Djossou F, Raoult D, Fournier PE - New Microbes New Infect (2015)

Transmission electron microscopy of Virgibacillus massiliensis strain Vm-5T, using Morgagni 268D transmission electron microscope (Philips/FEI, Hillsboro, OR, USA) at operating voltage of 60 kV. Scale bar = 500 nm. Arrow indicates terminal spore.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4644261&req=5

fig3: Transmission electron microscopy of Virgibacillus massiliensis strain Vm-5T, using Morgagni 268D transmission electron microscope (Philips/FEI, Hillsboro, OR, USA) at operating voltage of 60 kV. Scale bar = 500 nm. Arrow indicates terminal spore.
Mentions: Strain Vm-5 T was mesophilic and grew at temperatures ranging from 15 to 45°C, at an optimum temperature of 37°C. The isolate required NaCl for growth and grew at salinity ranging from 5 to 200 g/L of NaCl (optimum at 50 g/L). The optimal pH for growth was 7.5 (pH range 5 to 9). The growth of strain Vm-5T was tested under aerobic atmosphere, in the presence of 5% CO2 and in anaerobic and microaerophilic atmospheres created using GENbag anaer and GENbag microaer (bioMérieux, Marcy l’Etoile, France), respectively. The strain was strictly aerobic and grew in the presence of 5% CO2 but did not grow in microaerophilic or anaerobic atmosphere. The size (2 to 6 μm in length and 0.5 μm in diameter) and ultrastructure of cells were determined by negative staining transmission electron microscopy (Fig. 3).

Bottom Line: Here we describe its phenotypic characteristics and complete genome sequence.The 4 353 177 bp long genome exhibits a G + C content of 36.87% and contains 4394 protein-coding and 125 predicted RNA genes.Phylogenetically and genetically, strain Vm-c is a member of the genus Virgibacillus but is distinct enough to be classified as a new species.

View Article: PubMed Central - PubMed

Affiliation: Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UM 63, CNRS 7278, IRD 198, Inserm 1095, Institut Hospitalo-Universitaire Méditerranée-Infection, Faculté de médecine, Aix-Marseille Université, Marseille, France.

ABSTRACT
Strain Vm-5(T) was isolated from the stool specimen of a 10-year-old Amazonian boy. This bacterium is a Gram-positive, strictly aerobic rod, motile by a polar flagellum. Here we describe its phenotypic characteristics and complete genome sequence. The 4 353 177 bp long genome exhibits a G + C content of 36.87% and contains 4394 protein-coding and 125 predicted RNA genes. Phylogenetically and genetically, strain Vm-c is a member of the genus Virgibacillus but is distinct enough to be classified as a new species. We propose the creation of V. massiliensis sp. nov., whose type strain is strain Vm-5(T) (CSUR P971 = DSM 28587).

No MeSH data available.


Related in: MedlinePlus