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Resistance and integron characterization of Acinetobacter baumannii in a teaching hospital in Chongqing, China.

Huang C, Long Q, Qian K, Fu T, Zhang Z, Liao P, Xie J - New Microbes New Infect (2015)

Bottom Line: Susceptibility data showed strains carrying integrons were significantly more resistant to all tested antibiotics that strains lacking integrons.The major epidemic strains from intensive care unit patients belong to international clone 2.In conclusion, the presence of integrons was significantly associated with multiple drug resistance of A. baumannii in this hospital, and class I integron isolates bearing AadA2-HP-dfrA were the prevalent strain in this hospital.

View Article: PubMed Central - PubMed

Affiliation: Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Eco-Environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Beibei, China ; The Fifth People's Hospital of Chongqing, Nanan, China.

ABSTRACT
A total of 189 Acinetobacter baumannii isolates were collected in 2011 from a teaching hospital in Chongqing, China. Susceptibility data showed strains carrying integrons were significantly more resistant to all tested antibiotics that strains lacking integrons. Five types of gene cassettes belonging to class I integrons were identified in this study, and for the first time two types of gene cassettes belonging to class II integrons are reported. Most of the cassettes belong to a class I integron (136/144) encoding arr3, aacA4, dfrA17, aadA5, aadB, cat, blaOXA10 , aadA1, aadA2, dfrA and aacC1. Isolates contained a class I gene cassette; AadA2-HP-dfrA was the prevalent strain in this hospital. A class II integron was detected in eight strains, which contained the type IV fimbriae expression regulatory gene pilR and sulfate adenylyltransferase, suggesting a possible role in multidrug resistance. The major epidemic strains from intensive care unit patients belong to international clone 2. In conclusion, the presence of integrons was significantly associated with multiple drug resistance of A. baumannii in this hospital, and class I integron isolates bearing AadA2-HP-dfrA were the prevalent strain in this hospital.

No MeSH data available.


Related in: MedlinePlus

Repetitive PCR analysis of isolates from intensive care unit. Isolates with more than 80% similarity were considered related. Arrow indicates isolates processed by multilocus sequence typing analysis to determine genotypes of Acinetobacter baumannii.
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fig1: Repetitive PCR analysis of isolates from intensive care unit. Isolates with more than 80% similarity were considered related. Arrow indicates isolates processed by multilocus sequence typing analysis to determine genotypes of Acinetobacter baumannii.

Mentions: Because the ICU environment is the hardest hit with A. baumannii infection, molecular typing using rep-PCR and MLST were carried out in strains isolated from the ICU. Rep-PCR testing of ICU isolates showed that 16 of 19 isolated were closely related, with similarity scores of over 80% (Fig. 1). We assumed that isolates with closely linked rep-PCR results would be the same MLST type, so only five representative strains were processed for MLST typing. All five strains were consistent with sequence type (ST) 2, corresponding to European clone II [18].


Resistance and integron characterization of Acinetobacter baumannii in a teaching hospital in Chongqing, China.

Huang C, Long Q, Qian K, Fu T, Zhang Z, Liao P, Xie J - New Microbes New Infect (2015)

Repetitive PCR analysis of isolates from intensive care unit. Isolates with more than 80% similarity were considered related. Arrow indicates isolates processed by multilocus sequence typing analysis to determine genotypes of Acinetobacter baumannii.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4644259&req=5

fig1: Repetitive PCR analysis of isolates from intensive care unit. Isolates with more than 80% similarity were considered related. Arrow indicates isolates processed by multilocus sequence typing analysis to determine genotypes of Acinetobacter baumannii.
Mentions: Because the ICU environment is the hardest hit with A. baumannii infection, molecular typing using rep-PCR and MLST were carried out in strains isolated from the ICU. Rep-PCR testing of ICU isolates showed that 16 of 19 isolated were closely related, with similarity scores of over 80% (Fig. 1). We assumed that isolates with closely linked rep-PCR results would be the same MLST type, so only five representative strains were processed for MLST typing. All five strains were consistent with sequence type (ST) 2, corresponding to European clone II [18].

Bottom Line: Susceptibility data showed strains carrying integrons were significantly more resistant to all tested antibiotics that strains lacking integrons.The major epidemic strains from intensive care unit patients belong to international clone 2.In conclusion, the presence of integrons was significantly associated with multiple drug resistance of A. baumannii in this hospital, and class I integron isolates bearing AadA2-HP-dfrA were the prevalent strain in this hospital.

View Article: PubMed Central - PubMed

Affiliation: Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Eco-Environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Beibei, China ; The Fifth People's Hospital of Chongqing, Nanan, China.

ABSTRACT
A total of 189 Acinetobacter baumannii isolates were collected in 2011 from a teaching hospital in Chongqing, China. Susceptibility data showed strains carrying integrons were significantly more resistant to all tested antibiotics that strains lacking integrons. Five types of gene cassettes belonging to class I integrons were identified in this study, and for the first time two types of gene cassettes belonging to class II integrons are reported. Most of the cassettes belong to a class I integron (136/144) encoding arr3, aacA4, dfrA17, aadA5, aadB, cat, blaOXA10 , aadA1, aadA2, dfrA and aacC1. Isolates contained a class I gene cassette; AadA2-HP-dfrA was the prevalent strain in this hospital. A class II integron was detected in eight strains, which contained the type IV fimbriae expression regulatory gene pilR and sulfate adenylyltransferase, suggesting a possible role in multidrug resistance. The major epidemic strains from intensive care unit patients belong to international clone 2. In conclusion, the presence of integrons was significantly associated with multiple drug resistance of A. baumannii in this hospital, and class I integron isolates bearing AadA2-HP-dfrA were the prevalent strain in this hospital.

No MeSH data available.


Related in: MedlinePlus