Relaxation of Loaded ESCRT-III Spiral Springs Drives Membrane Deformation.
Bottom Line: We reasoned that Snf7 spirals could function as spiral springs.Furthermore, we observed that the elastic expansion of compressed Snf7 spirals generated an area difference between the two sides of the membrane and thus curvature.This spring-like activity underlies the driving force by which ESCRT-III could mediate membrane deformation and fission.
Affiliation: University of Geneva, Department of Biochemistry, quai Ernest Ansermet 30, 1211 Geneva 4, Switzerland.Show MeSH
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Mentions: Imaging is performed using an inverted microscope assembled by 3i (Intelligent Imaging Innovation, Denver, USA) and Nikon (Eclipse C1, Nikon, Tokyo, Japan). For SDC imaging, a 2-μm-thick volume stack (1 μm above and below the supported membrane) is acquired then rendered to 2D by maximum intensity projection. TIRF Imaging is performed using a motorized Nikon TIRF system. The number of molecules within Snf7 oligomers is estimated by calibrating the microscope with commercially available fluorescent DNA origamis (GATTA-Brightness 9R and 18R, GATTAquant, Braunschweig, Germany) (Figure S6).
Affiliation: University of Geneva, Department of Biochemistry, quai Ernest Ansermet 30, 1211 Geneva 4, Switzerland.