Relaxation of Loaded ESCRT-III Spiral Springs Drives Membrane Deformation.
Bottom Line: We reasoned that Snf7 spirals could function as spiral springs.Furthermore, we observed that the elastic expansion of compressed Snf7 spirals generated an area difference between the two sides of the membrane and thus curvature.This spring-like activity underlies the driving force by which ESCRT-III could mediate membrane deformation and fission.
Affiliation: University of Geneva, Department of Biochemistry, quai Ernest Ansermet 30, 1211 Geneva 4, Switzerland.Show MeSH
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Mentions: We started by reconstituting Snf7 polymerization on GUVs adhered to a glass surface, to avoid any displacement during time-lapse imaging (Figure S5A) and followed the increase of Snf7 fluorescence on the vesicle with time. In supported bilayers, which have smaller lipid mobility than free bilayers, diffusion of Snf7 assemblies is very limited. In contrast, Snf7 assemblies had a higher diffusion on GUVs explaining that fluorescence showed a homogeneous distribution (Figure 5C).
Affiliation: University of Geneva, Department of Biochemistry, quai Ernest Ansermet 30, 1211 Geneva 4, Switzerland.