Relaxation of Loaded ESCRT-III Spiral Springs Drives Membrane Deformation.
Bottom Line: We reasoned that Snf7 spirals could function as spiral springs.Furthermore, we observed that the elastic expansion of compressed Snf7 spirals generated an area difference between the two sides of the membrane and thus curvature.This spring-like activity underlies the driving force by which ESCRT-III could mediate membrane deformation and fission.
Affiliation: University of Geneva, Department of Biochemistry, quai Ernest Ansermet 30, 1211 Geneva 4, Switzerland.Show MeSH
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Mentions: To study the polymerization of ESCRT-III, we reconstituted ESCRT-III polymerization by adding purified yeast Snf7 onto supported lipid membranes. Supported membranes were obtained by bursting giant unilamellar vesicles (GUVs) composed of 40% di-oleoyl-phosphatidylserine (DOPS) and 60% of di-oleoyl-phosphatidylcholine (DOPC) on cleaned glass coverslips (Figure S1A, Movie S1). These coverslips were built into a flow chamber, allowing sequential addition and exchange of solutions.
Affiliation: University of Geneva, Department of Biochemistry, quai Ernest Ansermet 30, 1211 Geneva 4, Switzerland.