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Functional elucidation of miR-494 in the tumorigenesis of nasopharyngeal carcinoma.

Duan HF, Li XQ, Hu HY, Li YC, Cai Z, Mei XS, Yu P, Nie LP, Zhang W, Yu ZD, Nie GH - Tumour Biol. (2015)

Bottom Line: Nasopharyngeal carcinoma has very high incidence and high mortality worldwide.In the present study, we verify that the expression of miR-494 in NPC tissues and NPC-derived cells was down-regulated, respectively.The proliferation, colony formation, migration, and invasion of NPC-derived cells were suppressed, while the cell apoptosis was promoted, when miR-494 was over-expressed in these cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngological, Peking University Shenzhen Hospital, 518036, Shenzhen, Guangdong Province, China.

ABSTRACT
Nasopharyngeal carcinoma has very high incidence and high mortality worldwide. MiRNA is related to the tumorigenesis and metastasis of a variety of tumors. In the present study, we verify that the expression of miR-494 in NPC tissues and NPC-derived cells was down-regulated, respectively. The proliferation, colony formation, migration, and invasion of NPC-derived cells were suppressed, while the cell apoptosis was promoted, when miR-494 was over-expressed in these cells. GALNT7 and CDK16 were confirmed to be the direct targets of miR-494. These results suggested that miR-494 play an inhibitory role in the tumorigenesis of NPC.

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Related in: MedlinePlus

MiR-494 down-regulated the expression of GALNT7 and CDK16. a, b Western blotting showed that the function of miR-494 on GALNT7 and CDK16 expression at protein level in 6-10B, 9-4E, or CNE2 cells after transfection of miR-494 mimic, negative control, inhibitor, or inhibitor negative control. Date are presented as mean ± SD (*p < 0.05, **p < 0.001). c, d The mRNA expression level of GALNT7 and CDK16 negatively correlated with miR-494 in 30 NPC tumor samples by qRT-PCR. 2−ΔΔCT method was used to analyze the data
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Fig7: MiR-494 down-regulated the expression of GALNT7 and CDK16. a, b Western blotting showed that the function of miR-494 on GALNT7 and CDK16 expression at protein level in 6-10B, 9-4E, or CNE2 cells after transfection of miR-494 mimic, negative control, inhibitor, or inhibitor negative control. Date are presented as mean ± SD (*p < 0.05, **p < 0.001). c, d The mRNA expression level of GALNT7 and CDK16 negatively correlated with miR-494 in 30 NPC tumor samples by qRT-PCR. 2−ΔΔCT method was used to analyze the data

Mentions: The protein expression levels of GALNT7 or CDK16 were examined by Western blotting in 6-10B, 9-4E, and CNE2 cells. MiR-494 mimic significantly down-regulated the protein levels of GALNT7 and CDK16. However, miR-494 inhibitor did not show obvious effect on the levels of GALNT7 and CDK6 (Fig. 7a, b).Fig. 7


Functional elucidation of miR-494 in the tumorigenesis of nasopharyngeal carcinoma.

Duan HF, Li XQ, Hu HY, Li YC, Cai Z, Mei XS, Yu P, Nie LP, Zhang W, Yu ZD, Nie GH - Tumour Biol. (2015)

MiR-494 down-regulated the expression of GALNT7 and CDK16. a, b Western blotting showed that the function of miR-494 on GALNT7 and CDK16 expression at protein level in 6-10B, 9-4E, or CNE2 cells after transfection of miR-494 mimic, negative control, inhibitor, or inhibitor negative control. Date are presented as mean ± SD (*p < 0.05, **p < 0.001). c, d The mRNA expression level of GALNT7 and CDK16 negatively correlated with miR-494 in 30 NPC tumor samples by qRT-PCR. 2−ΔΔCT method was used to analyze the data
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4644213&req=5

Fig7: MiR-494 down-regulated the expression of GALNT7 and CDK16. a, b Western blotting showed that the function of miR-494 on GALNT7 and CDK16 expression at protein level in 6-10B, 9-4E, or CNE2 cells after transfection of miR-494 mimic, negative control, inhibitor, or inhibitor negative control. Date are presented as mean ± SD (*p < 0.05, **p < 0.001). c, d The mRNA expression level of GALNT7 and CDK16 negatively correlated with miR-494 in 30 NPC tumor samples by qRT-PCR. 2−ΔΔCT method was used to analyze the data
Mentions: The protein expression levels of GALNT7 or CDK16 were examined by Western blotting in 6-10B, 9-4E, and CNE2 cells. MiR-494 mimic significantly down-regulated the protein levels of GALNT7 and CDK16. However, miR-494 inhibitor did not show obvious effect on the levels of GALNT7 and CDK6 (Fig. 7a, b).Fig. 7

Bottom Line: Nasopharyngeal carcinoma has very high incidence and high mortality worldwide.In the present study, we verify that the expression of miR-494 in NPC tissues and NPC-derived cells was down-regulated, respectively.The proliferation, colony formation, migration, and invasion of NPC-derived cells were suppressed, while the cell apoptosis was promoted, when miR-494 was over-expressed in these cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngological, Peking University Shenzhen Hospital, 518036, Shenzhen, Guangdong Province, China.

ABSTRACT
Nasopharyngeal carcinoma has very high incidence and high mortality worldwide. MiRNA is related to the tumorigenesis and metastasis of a variety of tumors. In the present study, we verify that the expression of miR-494 in NPC tissues and NPC-derived cells was down-regulated, respectively. The proliferation, colony formation, migration, and invasion of NPC-derived cells were suppressed, while the cell apoptosis was promoted, when miR-494 was over-expressed in these cells. GALNT7 and CDK16 were confirmed to be the direct targets of miR-494. These results suggested that miR-494 play an inhibitory role in the tumorigenesis of NPC.

Show MeSH
Related in: MedlinePlus