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Functional elucidation of miR-494 in the tumorigenesis of nasopharyngeal carcinoma.

Duan HF, Li XQ, Hu HY, Li YC, Cai Z, Mei XS, Yu P, Nie LP, Zhang W, Yu ZD, Nie GH - Tumour Biol. (2015)

Bottom Line: Nasopharyngeal carcinoma has very high incidence and high mortality worldwide.In the present study, we verify that the expression of miR-494 in NPC tissues and NPC-derived cells was down-regulated, respectively.The proliferation, colony formation, migration, and invasion of NPC-derived cells were suppressed, while the cell apoptosis was promoted, when miR-494 was over-expressed in these cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngological, Peking University Shenzhen Hospital, 518036, Shenzhen, Guangdong Province, China.

ABSTRACT
Nasopharyngeal carcinoma has very high incidence and high mortality worldwide. MiRNA is related to the tumorigenesis and metastasis of a variety of tumors. In the present study, we verify that the expression of miR-494 in NPC tissues and NPC-derived cells was down-regulated, respectively. The proliferation, colony formation, migration, and invasion of NPC-derived cells were suppressed, while the cell apoptosis was promoted, when miR-494 was over-expressed in these cells. GALNT7 and CDK16 were confirmed to be the direct targets of miR-494. These results suggested that miR-494 play an inhibitory role in the tumorigenesis of NPC.

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Related in: MedlinePlus

Validation of cell transfection efficiency. a Phase-contrast and green fluorescence images were taken from the same field. b The relative expression levels of miR-494 in 6-10B, 9-4E, and CNE2 cells transfected with miR-494 mimic, negative control, miR-494 inhibitor, or inhibitor negative control. Data are presented as mean ± SD (*p < 0.05, **p < 0.001)
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Fig2: Validation of cell transfection efficiency. a Phase-contrast and green fluorescence images were taken from the same field. b The relative expression levels of miR-494 in 6-10B, 9-4E, and CNE2 cells transfected with miR-494 mimic, negative control, miR-494 inhibitor, or inhibitor negative control. Data are presented as mean ± SD (*p < 0.05, **p < 0.001)

Mentions: As shown in Fig. 2a, the transfection efficiency was more than 90 % when the cells were transfected with fluorescence-conjugated miRNA. qRT-PCR was used to verify the transfection effect and revealed that miR-494 was over-expressed obviously after transfection with miR-494 mimic in 6-10B (p < 0.001), 9-4E (p = 0.045), and CNE2 (p = 0.001) cells, and decreased after transfection with miR-494 inhibitor in 6-10B (p = 0.019), 9-4E (p = 0.005), and CNE2 (p = 0.010) cells (Fig. 2b).Fig. 2


Functional elucidation of miR-494 in the tumorigenesis of nasopharyngeal carcinoma.

Duan HF, Li XQ, Hu HY, Li YC, Cai Z, Mei XS, Yu P, Nie LP, Zhang W, Yu ZD, Nie GH - Tumour Biol. (2015)

Validation of cell transfection efficiency. a Phase-contrast and green fluorescence images were taken from the same field. b The relative expression levels of miR-494 in 6-10B, 9-4E, and CNE2 cells transfected with miR-494 mimic, negative control, miR-494 inhibitor, or inhibitor negative control. Data are presented as mean ± SD (*p < 0.05, **p < 0.001)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4644213&req=5

Fig2: Validation of cell transfection efficiency. a Phase-contrast and green fluorescence images were taken from the same field. b The relative expression levels of miR-494 in 6-10B, 9-4E, and CNE2 cells transfected with miR-494 mimic, negative control, miR-494 inhibitor, or inhibitor negative control. Data are presented as mean ± SD (*p < 0.05, **p < 0.001)
Mentions: As shown in Fig. 2a, the transfection efficiency was more than 90 % when the cells were transfected with fluorescence-conjugated miRNA. qRT-PCR was used to verify the transfection effect and revealed that miR-494 was over-expressed obviously after transfection with miR-494 mimic in 6-10B (p < 0.001), 9-4E (p = 0.045), and CNE2 (p = 0.001) cells, and decreased after transfection with miR-494 inhibitor in 6-10B (p = 0.019), 9-4E (p = 0.005), and CNE2 (p = 0.010) cells (Fig. 2b).Fig. 2

Bottom Line: Nasopharyngeal carcinoma has very high incidence and high mortality worldwide.In the present study, we verify that the expression of miR-494 in NPC tissues and NPC-derived cells was down-regulated, respectively.The proliferation, colony formation, migration, and invasion of NPC-derived cells were suppressed, while the cell apoptosis was promoted, when miR-494 was over-expressed in these cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngological, Peking University Shenzhen Hospital, 518036, Shenzhen, Guangdong Province, China.

ABSTRACT
Nasopharyngeal carcinoma has very high incidence and high mortality worldwide. MiRNA is related to the tumorigenesis and metastasis of a variety of tumors. In the present study, we verify that the expression of miR-494 in NPC tissues and NPC-derived cells was down-regulated, respectively. The proliferation, colony formation, migration, and invasion of NPC-derived cells were suppressed, while the cell apoptosis was promoted, when miR-494 was over-expressed in these cells. GALNT7 and CDK16 were confirmed to be the direct targets of miR-494. These results suggested that miR-494 play an inhibitory role in the tumorigenesis of NPC.

Show MeSH
Related in: MedlinePlus