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Functional elucidation of miR-494 in the tumorigenesis of nasopharyngeal carcinoma.

Duan HF, Li XQ, Hu HY, Li YC, Cai Z, Mei XS, Yu P, Nie LP, Zhang W, Yu ZD, Nie GH - Tumour Biol. (2015)

Bottom Line: Nasopharyngeal carcinoma has very high incidence and high mortality worldwide.In the present study, we verify that the expression of miR-494 in NPC tissues and NPC-derived cells was down-regulated, respectively.The proliferation, colony formation, migration, and invasion of NPC-derived cells were suppressed, while the cell apoptosis was promoted, when miR-494 was over-expressed in these cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngological, Peking University Shenzhen Hospital, 518036, Shenzhen, Guangdong Province, China.

ABSTRACT
Nasopharyngeal carcinoma has very high incidence and high mortality worldwide. MiRNA is related to the tumorigenesis and metastasis of a variety of tumors. In the present study, we verify that the expression of miR-494 in NPC tissues and NPC-derived cells was down-regulated, respectively. The proliferation, colony formation, migration, and invasion of NPC-derived cells were suppressed, while the cell apoptosis was promoted, when miR-494 was over-expressed in these cells. GALNT7 and CDK16 were confirmed to be the direct targets of miR-494. These results suggested that miR-494 play an inhibitory role in the tumorigenesis of NPC.

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Related in: MedlinePlus

The expression of miR-494 in NPC tissues and NPC-derived cells. a MiR-494 is down-regulated in NPC qRT-PCR of miR-494 expression relative to U6 expression in 20 NPC tumor samples compared to 20 normal nasopharyngeal epithelial tissues. b qRT-PCR showed that miR-494 was down-regulated in 6-10B, 9-4E, and CNE2 cells compared with NP69 cell. 2−ΔΔCT method was used to analyze the data, and the data are presented as mean ± SD (*p < 0.05, **p < 0.001)
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Fig1: The expression of miR-494 in NPC tissues and NPC-derived cells. a MiR-494 is down-regulated in NPC qRT-PCR of miR-494 expression relative to U6 expression in 20 NPC tumor samples compared to 20 normal nasopharyngeal epithelial tissues. b qRT-PCR showed that miR-494 was down-regulated in 6-10B, 9-4E, and CNE2 cells compared with NP69 cell. 2−ΔΔCT method was used to analyze the data, and the data are presented as mean ± SD (*p < 0.05, **p < 0.001)

Mentions: The expression of miR-494 was examined in 20 freshly frozen NPC clinical specimens and 20 normal nasopharyngeal epithelial tissues. As shown in Fig. 1a, the expression of miR-494 was significantly down-regulated in NPC tissues compared with that in normal nasopharyngeal epithelial tissues.Fig. 1


Functional elucidation of miR-494 in the tumorigenesis of nasopharyngeal carcinoma.

Duan HF, Li XQ, Hu HY, Li YC, Cai Z, Mei XS, Yu P, Nie LP, Zhang W, Yu ZD, Nie GH - Tumour Biol. (2015)

The expression of miR-494 in NPC tissues and NPC-derived cells. a MiR-494 is down-regulated in NPC qRT-PCR of miR-494 expression relative to U6 expression in 20 NPC tumor samples compared to 20 normal nasopharyngeal epithelial tissues. b qRT-PCR showed that miR-494 was down-regulated in 6-10B, 9-4E, and CNE2 cells compared with NP69 cell. 2−ΔΔCT method was used to analyze the data, and the data are presented as mean ± SD (*p < 0.05, **p < 0.001)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4644213&req=5

Fig1: The expression of miR-494 in NPC tissues and NPC-derived cells. a MiR-494 is down-regulated in NPC qRT-PCR of miR-494 expression relative to U6 expression in 20 NPC tumor samples compared to 20 normal nasopharyngeal epithelial tissues. b qRT-PCR showed that miR-494 was down-regulated in 6-10B, 9-4E, and CNE2 cells compared with NP69 cell. 2−ΔΔCT method was used to analyze the data, and the data are presented as mean ± SD (*p < 0.05, **p < 0.001)
Mentions: The expression of miR-494 was examined in 20 freshly frozen NPC clinical specimens and 20 normal nasopharyngeal epithelial tissues. As shown in Fig. 1a, the expression of miR-494 was significantly down-regulated in NPC tissues compared with that in normal nasopharyngeal epithelial tissues.Fig. 1

Bottom Line: Nasopharyngeal carcinoma has very high incidence and high mortality worldwide.In the present study, we verify that the expression of miR-494 in NPC tissues and NPC-derived cells was down-regulated, respectively.The proliferation, colony formation, migration, and invasion of NPC-derived cells were suppressed, while the cell apoptosis was promoted, when miR-494 was over-expressed in these cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngological, Peking University Shenzhen Hospital, 518036, Shenzhen, Guangdong Province, China.

ABSTRACT
Nasopharyngeal carcinoma has very high incidence and high mortality worldwide. MiRNA is related to the tumorigenesis and metastasis of a variety of tumors. In the present study, we verify that the expression of miR-494 in NPC tissues and NPC-derived cells was down-regulated, respectively. The proliferation, colony formation, migration, and invasion of NPC-derived cells were suppressed, while the cell apoptosis was promoted, when miR-494 was over-expressed in these cells. GALNT7 and CDK16 were confirmed to be the direct targets of miR-494. These results suggested that miR-494 play an inhibitory role in the tumorigenesis of NPC.

Show MeSH
Related in: MedlinePlus