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ALDH1 might influence the metastatic capability of HeLa cells.

Yao T, Lu R, Li Y, Peng Y, Ding M, Xie X, Lin Z - Tumour Biol. (2015)

Bottom Line: We showed that knockdown of ALDH1 expression reduced the cell migration ability of HeLa cells, whereas augmented expression of ALDH1 increased cell migration.However, there was no difference in the cellular proliferation, apoptosis, cell cycle, and invasion.These results indicate that ALDH1 is directly involved in HeLa migration.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecological Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 107 Yan Jiang West Road, Guangzhou, 510120, People's Republic of China. yaoyaotingting@msn.com.

ABSTRACT
Recent data suggest that tumor persistence and recurrence could be caused by the presence of cancer stem cells (CSCs). Aldehyde dehydrogenase 1 (ALDH1) has been implicated in cancer pathogenesis and used as a CSC marker. We previously reported that cervical carcinoma contains a small subpopulation of cells expressing ALDH1 [1]. In this study, we used small interfering RNA to suppress ALDH1 expression and introduced an ALDH1 reporting vector into HeLa cells followed by various in vitro assays. We showed that knockdown of ALDH1 expression reduced the cell migration ability of HeLa cells, whereas augmented expression of ALDH1 increased cell migration. However, there was no difference in the cellular proliferation, apoptosis, cell cycle, and invasion. These results indicate that ALDH1 is directly involved in HeLa migration.

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The Transwell analysis of migration. a The cells without any treatment, cells with non-targeting siRNA and cells with siRNA targeting the specific sequence. b The cells without any treatment, cells just with vector and cells with pIRES2/ALDH1 construct. c Each bar represents mean values ± SE from three independent experiments for cells without any treatment, with non-targeting siRNA and with siRNA targeting the specific sequence. d Each bar represents mean values ± SE from three independent experiments for cells without any treatment, cells just with vector and cells with pIRES2/ALDH1 construct
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Fig5: The Transwell analysis of migration. a The cells without any treatment, cells with non-targeting siRNA and cells with siRNA targeting the specific sequence. b The cells without any treatment, cells just with vector and cells with pIRES2/ALDH1 construct. c Each bar represents mean values ± SE from three independent experiments for cells without any treatment, with non-targeting siRNA and with siRNA targeting the specific sequence. d Each bar represents mean values ± SE from three independent experiments for cells without any treatment, cells just with vector and cells with pIRES2/ALDH1 construct

Mentions: To examine the effect of ALDH1 on the mobility of HeLa cells, the migration was measured by Transwell assays. We showed that the SiRNA-transfected HeLa cells had lower migration rates than the control (Fig. 5a, c), whereas the HeLa-ALDH1-positive cells had significantly higher migration rates than their control cells (Fig. 5b, d), suggesting that ALDH1 is an effective target for treating cervical cancer metastases.Fig. 5


ALDH1 might influence the metastatic capability of HeLa cells.

Yao T, Lu R, Li Y, Peng Y, Ding M, Xie X, Lin Z - Tumour Biol. (2015)

The Transwell analysis of migration. a The cells without any treatment, cells with non-targeting siRNA and cells with siRNA targeting the specific sequence. b The cells without any treatment, cells just with vector and cells with pIRES2/ALDH1 construct. c Each bar represents mean values ± SE from three independent experiments for cells without any treatment, with non-targeting siRNA and with siRNA targeting the specific sequence. d Each bar represents mean values ± SE from three independent experiments for cells without any treatment, cells just with vector and cells with pIRES2/ALDH1 construct
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4644206&req=5

Fig5: The Transwell analysis of migration. a The cells without any treatment, cells with non-targeting siRNA and cells with siRNA targeting the specific sequence. b The cells without any treatment, cells just with vector and cells with pIRES2/ALDH1 construct. c Each bar represents mean values ± SE from three independent experiments for cells without any treatment, with non-targeting siRNA and with siRNA targeting the specific sequence. d Each bar represents mean values ± SE from three independent experiments for cells without any treatment, cells just with vector and cells with pIRES2/ALDH1 construct
Mentions: To examine the effect of ALDH1 on the mobility of HeLa cells, the migration was measured by Transwell assays. We showed that the SiRNA-transfected HeLa cells had lower migration rates than the control (Fig. 5a, c), whereas the HeLa-ALDH1-positive cells had significantly higher migration rates than their control cells (Fig. 5b, d), suggesting that ALDH1 is an effective target for treating cervical cancer metastases.Fig. 5

Bottom Line: We showed that knockdown of ALDH1 expression reduced the cell migration ability of HeLa cells, whereas augmented expression of ALDH1 increased cell migration.However, there was no difference in the cellular proliferation, apoptosis, cell cycle, and invasion.These results indicate that ALDH1 is directly involved in HeLa migration.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecological Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 107 Yan Jiang West Road, Guangzhou, 510120, People's Republic of China. yaoyaotingting@msn.com.

ABSTRACT
Recent data suggest that tumor persistence and recurrence could be caused by the presence of cancer stem cells (CSCs). Aldehyde dehydrogenase 1 (ALDH1) has been implicated in cancer pathogenesis and used as a CSC marker. We previously reported that cervical carcinoma contains a small subpopulation of cells expressing ALDH1 [1]. In this study, we used small interfering RNA to suppress ALDH1 expression and introduced an ALDH1 reporting vector into HeLa cells followed by various in vitro assays. We showed that knockdown of ALDH1 expression reduced the cell migration ability of HeLa cells, whereas augmented expression of ALDH1 increased cell migration. However, there was no difference in the cellular proliferation, apoptosis, cell cycle, and invasion. These results indicate that ALDH1 is directly involved in HeLa migration.

Show MeSH
Related in: MedlinePlus