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ALDH1 might influence the metastatic capability of HeLa cells.

Yao T, Lu R, Li Y, Peng Y, Ding M, Xie X, Lin Z - Tumour Biol. (2015)

Bottom Line: We showed that knockdown of ALDH1 expression reduced the cell migration ability of HeLa cells, whereas augmented expression of ALDH1 increased cell migration.However, there was no difference in the cellular proliferation, apoptosis, cell cycle, and invasion.These results indicate that ALDH1 is directly involved in HeLa migration.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecological Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 107 Yan Jiang West Road, Guangzhou, 510120, People's Republic of China. yaoyaotingting@msn.com.

ABSTRACT
Recent data suggest that tumor persistence and recurrence could be caused by the presence of cancer stem cells (CSCs). Aldehyde dehydrogenase 1 (ALDH1) has been implicated in cancer pathogenesis and used as a CSC marker. We previously reported that cervical carcinoma contains a small subpopulation of cells expressing ALDH1 [1]. In this study, we used small interfering RNA to suppress ALDH1 expression and introduced an ALDH1 reporting vector into HeLa cells followed by various in vitro assays. We showed that knockdown of ALDH1 expression reduced the cell migration ability of HeLa cells, whereas augmented expression of ALDH1 increased cell migration. However, there was no difference in the cellular proliferation, apoptosis, cell cycle, and invasion. These results indicate that ALDH1 is directly involved in HeLa migration.

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The MTT assay to evaluate cell proliferation. a The OD value of the cells without any treatment, the cells with non-targeting of siRNA and the cells with siRNA targeting the specific sequence. b The OD value of the cells without treatment, the cells with vector and the cells with a pIRES2/ALDH1 construct
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Fig2: The MTT assay to evaluate cell proliferation. a The OD value of the cells without any treatment, the cells with non-targeting of siRNA and the cells with siRNA targeting the specific sequence. b The OD value of the cells without treatment, the cells with vector and the cells with a pIRES2/ALDH1 construct

Mentions: As shown in Fig. 2a, knockdown of ALDH1 using the oligonuclear sequences resulted in no significant decrease and no significant increase in the HeLa cell proliferation (Fig. 2a), apoptosis (Fig. 3a, c), and cell cycle (Fig. 3a) compared to the empty vector-transfected cells. Similarly, no obvious alteration of the HeLa cells was found in the ALDH1 overexpressing cells in comparison to that in the control sequence-transfected group (Figs. 2b, 3b, c, and 4b).Fig. 2


ALDH1 might influence the metastatic capability of HeLa cells.

Yao T, Lu R, Li Y, Peng Y, Ding M, Xie X, Lin Z - Tumour Biol. (2015)

The MTT assay to evaluate cell proliferation. a The OD value of the cells without any treatment, the cells with non-targeting of siRNA and the cells with siRNA targeting the specific sequence. b The OD value of the cells without treatment, the cells with vector and the cells with a pIRES2/ALDH1 construct
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4644206&req=5

Fig2: The MTT assay to evaluate cell proliferation. a The OD value of the cells without any treatment, the cells with non-targeting of siRNA and the cells with siRNA targeting the specific sequence. b The OD value of the cells without treatment, the cells with vector and the cells with a pIRES2/ALDH1 construct
Mentions: As shown in Fig. 2a, knockdown of ALDH1 using the oligonuclear sequences resulted in no significant decrease and no significant increase in the HeLa cell proliferation (Fig. 2a), apoptosis (Fig. 3a, c), and cell cycle (Fig. 3a) compared to the empty vector-transfected cells. Similarly, no obvious alteration of the HeLa cells was found in the ALDH1 overexpressing cells in comparison to that in the control sequence-transfected group (Figs. 2b, 3b, c, and 4b).Fig. 2

Bottom Line: We showed that knockdown of ALDH1 expression reduced the cell migration ability of HeLa cells, whereas augmented expression of ALDH1 increased cell migration.However, there was no difference in the cellular proliferation, apoptosis, cell cycle, and invasion.These results indicate that ALDH1 is directly involved in HeLa migration.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecological Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 107 Yan Jiang West Road, Guangzhou, 510120, People's Republic of China. yaoyaotingting@msn.com.

ABSTRACT
Recent data suggest that tumor persistence and recurrence could be caused by the presence of cancer stem cells (CSCs). Aldehyde dehydrogenase 1 (ALDH1) has been implicated in cancer pathogenesis and used as a CSC marker. We previously reported that cervical carcinoma contains a small subpopulation of cells expressing ALDH1 [1]. In this study, we used small interfering RNA to suppress ALDH1 expression and introduced an ALDH1 reporting vector into HeLa cells followed by various in vitro assays. We showed that knockdown of ALDH1 expression reduced the cell migration ability of HeLa cells, whereas augmented expression of ALDH1 increased cell migration. However, there was no difference in the cellular proliferation, apoptosis, cell cycle, and invasion. These results indicate that ALDH1 is directly involved in HeLa migration.

Show MeSH
Related in: MedlinePlus