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ALDH1 might influence the metastatic capability of HeLa cells.

Yao T, Lu R, Li Y, Peng Y, Ding M, Xie X, Lin Z - Tumour Biol. (2015)

Bottom Line: We showed that knockdown of ALDH1 expression reduced the cell migration ability of HeLa cells, whereas augmented expression of ALDH1 increased cell migration.However, there was no difference in the cellular proliferation, apoptosis, cell cycle, and invasion.These results indicate that ALDH1 is directly involved in HeLa migration.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecological Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 107 Yan Jiang West Road, Guangzhou, 510120, People's Republic of China. yaoyaotingting@msn.com.

ABSTRACT
Recent data suggest that tumor persistence and recurrence could be caused by the presence of cancer stem cells (CSCs). Aldehyde dehydrogenase 1 (ALDH1) has been implicated in cancer pathogenesis and used as a CSC marker. We previously reported that cervical carcinoma contains a small subpopulation of cells expressing ALDH1 [1]. In this study, we used small interfering RNA to suppress ALDH1 expression and introduced an ALDH1 reporting vector into HeLa cells followed by various in vitro assays. We showed that knockdown of ALDH1 expression reduced the cell migration ability of HeLa cells, whereas augmented expression of ALDH1 increased cell migration. However, there was no difference in the cellular proliferation, apoptosis, cell cycle, and invasion. These results indicate that ALDH1 is directly involved in HeLa migration.

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Expression of ALDH1 in various cells. a The mRNA expression of ALDH1 in the cells without treatment, the cells with non-targeting siRNA and the cells with siRNA targeting the specific sequence. b The detection of ALDH1 mRNA levels in cells without any treatment, the cells just with vector and cells with a pIRES2/ALDH1 construct
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Fig1: Expression of ALDH1 in various cells. a The mRNA expression of ALDH1 in the cells without treatment, the cells with non-targeting siRNA and the cells with siRNA targeting the specific sequence. b The detection of ALDH1 mRNA levels in cells without any treatment, the cells just with vector and cells with a pIRES2/ALDH1 construct

Mentions: RNAi technology was employed to silence the ALDH1 expression. Real-time PCR and Western blot were performed to quantify ALDH1 expression. Endogenous ALDH1 mRNA expression (Fig. 1a) were significantly lowered by RNAi (HeLa-ALDH1-RNAi) than by the control transfections (HeLa-NCi) and HeLa cells. To increase the ALDH1 expression, the cells were stably transfected with an ALDH1 expression construct. After transfection and G418 selection, the ALDH1 mRNA (Fig. 1b) expression levels in the stably transfected cells (HeLa-ALDH1) were much higher than in the HeLa cells.Fig. 1


ALDH1 might influence the metastatic capability of HeLa cells.

Yao T, Lu R, Li Y, Peng Y, Ding M, Xie X, Lin Z - Tumour Biol. (2015)

Expression of ALDH1 in various cells. a The mRNA expression of ALDH1 in the cells without treatment, the cells with non-targeting siRNA and the cells with siRNA targeting the specific sequence. b The detection of ALDH1 mRNA levels in cells without any treatment, the cells just with vector and cells with a pIRES2/ALDH1 construct
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4644206&req=5

Fig1: Expression of ALDH1 in various cells. a The mRNA expression of ALDH1 in the cells without treatment, the cells with non-targeting siRNA and the cells with siRNA targeting the specific sequence. b The detection of ALDH1 mRNA levels in cells without any treatment, the cells just with vector and cells with a pIRES2/ALDH1 construct
Mentions: RNAi technology was employed to silence the ALDH1 expression. Real-time PCR and Western blot were performed to quantify ALDH1 expression. Endogenous ALDH1 mRNA expression (Fig. 1a) were significantly lowered by RNAi (HeLa-ALDH1-RNAi) than by the control transfections (HeLa-NCi) and HeLa cells. To increase the ALDH1 expression, the cells were stably transfected with an ALDH1 expression construct. After transfection and G418 selection, the ALDH1 mRNA (Fig. 1b) expression levels in the stably transfected cells (HeLa-ALDH1) were much higher than in the HeLa cells.Fig. 1

Bottom Line: We showed that knockdown of ALDH1 expression reduced the cell migration ability of HeLa cells, whereas augmented expression of ALDH1 increased cell migration.However, there was no difference in the cellular proliferation, apoptosis, cell cycle, and invasion.These results indicate that ALDH1 is directly involved in HeLa migration.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecological Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 107 Yan Jiang West Road, Guangzhou, 510120, People's Republic of China. yaoyaotingting@msn.com.

ABSTRACT
Recent data suggest that tumor persistence and recurrence could be caused by the presence of cancer stem cells (CSCs). Aldehyde dehydrogenase 1 (ALDH1) has been implicated in cancer pathogenesis and used as a CSC marker. We previously reported that cervical carcinoma contains a small subpopulation of cells expressing ALDH1 [1]. In this study, we used small interfering RNA to suppress ALDH1 expression and introduced an ALDH1 reporting vector into HeLa cells followed by various in vitro assays. We showed that knockdown of ALDH1 expression reduced the cell migration ability of HeLa cells, whereas augmented expression of ALDH1 increased cell migration. However, there was no difference in the cellular proliferation, apoptosis, cell cycle, and invasion. These results indicate that ALDH1 is directly involved in HeLa migration.

Show MeSH
Related in: MedlinePlus