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MicroRNA-199a-3p suppresses glioma cell proliferation by regulating the AKT/mTOR signaling pathway.

Shen L, Sun C, Li Y, Li X, Sun T, Liu C, Zhou Y, Du Z - Tumour Biol. (2015)

Bottom Line: The over-expression of miR-199a-3p might target mTOR and restrained cellular growth and proliferation but not invasive and apoptosis capability.Results indicated that cellular proliferation was inhibited to regulate the AKT/mTOR signaling pathway by elevating levels of miR-199a-3p.MiR-199a-3p in glioma cell lines has effects similar to the tumor suppressor gene on cellular proliferation via the AKT/mTOR signaling pathway.

View Article: PubMed Central - PubMed

Affiliation: Neurosurgery and Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, Jiangsu, 215006, People's Republic of China.

ABSTRACT
Glioma has been investigated for decades, but the prognosis remains poor because of rapid proliferation, its aggressive potential, and its resistance to chemotherapy or radiotherapy. The mammalian target of rapamycin (mTOR) is highly expressed and regulates cellular proliferation and cell growth. MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene transcription and translation via up-regulating or down-regulating the levels of miRNAs. This study was conducted to explore the molecular functions of miR-199a-3p in glioma. We detected the expression of miR-199a-3p in glioma samples by quantitative PCR (qPCR). Then, we transfected the U87 and U251 cell lines with miR-199a-3p. Cellular proliferation, invasion, and apoptosis were assessed to explain the function of miR-199a-3p. PCR confirmed that the expression of miR-199a-3p was lower in glioma samples combined with normal brain tissues. The over-expression of miR-199a-3p might target mTOR and restrained cellular growth and proliferation but not invasive and apoptosis capability. Results indicated that cellular proliferation was inhibited to regulate the AKT/mTOR signaling pathway by elevating levels of miR-199a-3p. MiR-199a-3p in glioma cell lines has effects similar to the tumor suppressor gene on cellular proliferation via the AKT/mTOR signaling pathway.

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The invasion and apoptosis were assessed and compared with miR-199a-3p mimic negative control. a, b Invasion ability was conducted by transwell invasion system. The number of invasive cells was counted (ampersand, P > 0.05). c, d Apoptosis capability was detected by flow cytometry analysis (ampersand, P > 0.05). e The expression of MMP2 and caspase-3 were conducted (ampersand, P > 0.05)
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Fig4: The invasion and apoptosis were assessed and compared with miR-199a-3p mimic negative control. a, b Invasion ability was conducted by transwell invasion system. The number of invasive cells was counted (ampersand, P > 0.05). c, d Apoptosis capability was detected by flow cytometry analysis (ampersand, P > 0.05). e The expression of MMP2 and caspase-3 were conducted (ampersand, P > 0.05)

Mentions: To determine whether the suppression of cellular proliferation was due to decreased invasion capability or increased apoptosis by the over-expression of miR-199a-3p, we carried out a cell invasion assay and apoptosis assay in vitro. At the same time, we detected the expression of MMP2 and caspase-3, which were related to invasion and apoptosis capability, by Western blot analysis. Results indicated that no significant difference was found between glioma cells’ over-expression of miR-199a-3p and those with negative control oligonucleotide (P > 0.05; Fig. 4).Fig. 4


MicroRNA-199a-3p suppresses glioma cell proliferation by regulating the AKT/mTOR signaling pathway.

Shen L, Sun C, Li Y, Li X, Sun T, Liu C, Zhou Y, Du Z - Tumour Biol. (2015)

The invasion and apoptosis were assessed and compared with miR-199a-3p mimic negative control. a, b Invasion ability was conducted by transwell invasion system. The number of invasive cells was counted (ampersand, P > 0.05). c, d Apoptosis capability was detected by flow cytometry analysis (ampersand, P > 0.05). e The expression of MMP2 and caspase-3 were conducted (ampersand, P > 0.05)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4644202&req=5

Fig4: The invasion and apoptosis were assessed and compared with miR-199a-3p mimic negative control. a, b Invasion ability was conducted by transwell invasion system. The number of invasive cells was counted (ampersand, P > 0.05). c, d Apoptosis capability was detected by flow cytometry analysis (ampersand, P > 0.05). e The expression of MMP2 and caspase-3 were conducted (ampersand, P > 0.05)
Mentions: To determine whether the suppression of cellular proliferation was due to decreased invasion capability or increased apoptosis by the over-expression of miR-199a-3p, we carried out a cell invasion assay and apoptosis assay in vitro. At the same time, we detected the expression of MMP2 and caspase-3, which were related to invasion and apoptosis capability, by Western blot analysis. Results indicated that no significant difference was found between glioma cells’ over-expression of miR-199a-3p and those with negative control oligonucleotide (P > 0.05; Fig. 4).Fig. 4

Bottom Line: The over-expression of miR-199a-3p might target mTOR and restrained cellular growth and proliferation but not invasive and apoptosis capability.Results indicated that cellular proliferation was inhibited to regulate the AKT/mTOR signaling pathway by elevating levels of miR-199a-3p.MiR-199a-3p in glioma cell lines has effects similar to the tumor suppressor gene on cellular proliferation via the AKT/mTOR signaling pathway.

View Article: PubMed Central - PubMed

Affiliation: Neurosurgery and Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, Jiangsu, 215006, People's Republic of China.

ABSTRACT
Glioma has been investigated for decades, but the prognosis remains poor because of rapid proliferation, its aggressive potential, and its resistance to chemotherapy or radiotherapy. The mammalian target of rapamycin (mTOR) is highly expressed and regulates cellular proliferation and cell growth. MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene transcription and translation via up-regulating or down-regulating the levels of miRNAs. This study was conducted to explore the molecular functions of miR-199a-3p in glioma. We detected the expression of miR-199a-3p in glioma samples by quantitative PCR (qPCR). Then, we transfected the U87 and U251 cell lines with miR-199a-3p. Cellular proliferation, invasion, and apoptosis were assessed to explain the function of miR-199a-3p. PCR confirmed that the expression of miR-199a-3p was lower in glioma samples combined with normal brain tissues. The over-expression of miR-199a-3p might target mTOR and restrained cellular growth and proliferation but not invasive and apoptosis capability. Results indicated that cellular proliferation was inhibited to regulate the AKT/mTOR signaling pathway by elevating levels of miR-199a-3p. MiR-199a-3p in glioma cell lines has effects similar to the tumor suppressor gene on cellular proliferation via the AKT/mTOR signaling pathway.

Show MeSH
Related in: MedlinePlus