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MicroRNA-199a-3p suppresses glioma cell proliferation by regulating the AKT/mTOR signaling pathway.

Shen L, Sun C, Li Y, Li X, Sun T, Liu C, Zhou Y, Du Z - Tumour Biol. (2015)

Bottom Line: The over-expression of miR-199a-3p might target mTOR and restrained cellular growth and proliferation but not invasive and apoptosis capability.Results indicated that cellular proliferation was inhibited to regulate the AKT/mTOR signaling pathway by elevating levels of miR-199a-3p.MiR-199a-3p in glioma cell lines has effects similar to the tumor suppressor gene on cellular proliferation via the AKT/mTOR signaling pathway.

View Article: PubMed Central - PubMed

Affiliation: Neurosurgery and Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, Jiangsu, 215006, People's Republic of China.

ABSTRACT
Glioma has been investigated for decades, but the prognosis remains poor because of rapid proliferation, its aggressive potential, and its resistance to chemotherapy or radiotherapy. The mammalian target of rapamycin (mTOR) is highly expressed and regulates cellular proliferation and cell growth. MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene transcription and translation via up-regulating or down-regulating the levels of miRNAs. This study was conducted to explore the molecular functions of miR-199a-3p in glioma. We detected the expression of miR-199a-3p in glioma samples by quantitative PCR (qPCR). Then, we transfected the U87 and U251 cell lines with miR-199a-3p. Cellular proliferation, invasion, and apoptosis were assessed to explain the function of miR-199a-3p. PCR confirmed that the expression of miR-199a-3p was lower in glioma samples combined with normal brain tissues. The over-expression of miR-199a-3p might target mTOR and restrained cellular growth and proliferation but not invasive and apoptosis capability. Results indicated that cellular proliferation was inhibited to regulate the AKT/mTOR signaling pathway by elevating levels of miR-199a-3p. MiR-199a-3p in glioma cell lines has effects similar to the tumor suppressor gene on cellular proliferation via the AKT/mTOR signaling pathway.

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a The expression of miR-199a-3p in U87 and U251 compared with six normal brain tissues (asterisks, P < 0.01). b The expression of miR-199a-3p in cells transfected with miR-199a-3p (asterisks, P < 0.01). c Cellular proliferation was detected in U87 and U251 by cell counting kit (CCK-8) after 6, 24, 48, and 72 h of incubation. Data were reported as means ± SD (asterisks, P < 0.01), compared with cells transfected with miR-199a-3p mimic negative control or untransfected cells. d mTOR in U87 and U251 with miR-199a-3p over-expression compared with miR-199a-3p mimic negative control (asterisks, P < 0.01). e Cell cycle modulation was restrained resulting in G1 phase increase (asterisks, P < 0.01) and S phase decrease (asterisk, P < 0.05)
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Fig2: a The expression of miR-199a-3p in U87 and U251 compared with six normal brain tissues (asterisks, P < 0.01). b The expression of miR-199a-3p in cells transfected with miR-199a-3p (asterisks, P < 0.01). c Cellular proliferation was detected in U87 and U251 by cell counting kit (CCK-8) after 6, 24, 48, and 72 h of incubation. Data were reported as means ± SD (asterisks, P < 0.01), compared with cells transfected with miR-199a-3p mimic negative control or untransfected cells. d mTOR in U87 and U251 with miR-199a-3p over-expression compared with miR-199a-3p mimic negative control (asterisks, P < 0.01). e Cell cycle modulation was restrained resulting in G1 phase increase (asterisks, P < 0.01) and S phase decrease (asterisk, P < 0.05)

Mentions: To gain further insight into the expression of miR-199a-3p in glioma samples and normal brain tissues, quantitative polymerase chain reaction (qPCR) was conducted in 6 normal brain tissues and 61 glioma samples. Results showed that the expression of miR-199a-3p was lower in glioma samples than normal brain tissues (P < 0.01; Fig. 1a), and there was no significant difference between low-grade glioma (grade II) and high-grade (grade III, grade IV) glioma (P > 0.05; Fig. 1a). The expression of mTOR in glioma samples was higher combined with normal brain tissues and increased with the increasing degree of malignancy in glioma (low-grade glioma vs. high-grade glioma, P < 0.01; Fig. 1b). In addition, RhoA and p70S6k expressions in glioma samples were higher than normal brain tissues (P < 0.01; Fig. 1c, d) and had no significant association in low-grade and high-grade glioma samples (P > 0.05; Fig. 1c, d). The expression of miR-199a-3p in U87 and U251 cell lines was very low (P < 0.01; Fig. 2a).Fig. 1


MicroRNA-199a-3p suppresses glioma cell proliferation by regulating the AKT/mTOR signaling pathway.

Shen L, Sun C, Li Y, Li X, Sun T, Liu C, Zhou Y, Du Z - Tumour Biol. (2015)

a The expression of miR-199a-3p in U87 and U251 compared with six normal brain tissues (asterisks, P < 0.01). b The expression of miR-199a-3p in cells transfected with miR-199a-3p (asterisks, P < 0.01). c Cellular proliferation was detected in U87 and U251 by cell counting kit (CCK-8) after 6, 24, 48, and 72 h of incubation. Data were reported as means ± SD (asterisks, P < 0.01), compared with cells transfected with miR-199a-3p mimic negative control or untransfected cells. d mTOR in U87 and U251 with miR-199a-3p over-expression compared with miR-199a-3p mimic negative control (asterisks, P < 0.01). e Cell cycle modulation was restrained resulting in G1 phase increase (asterisks, P < 0.01) and S phase decrease (asterisk, P < 0.05)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4644202&req=5

Fig2: a The expression of miR-199a-3p in U87 and U251 compared with six normal brain tissues (asterisks, P < 0.01). b The expression of miR-199a-3p in cells transfected with miR-199a-3p (asterisks, P < 0.01). c Cellular proliferation was detected in U87 and U251 by cell counting kit (CCK-8) after 6, 24, 48, and 72 h of incubation. Data were reported as means ± SD (asterisks, P < 0.01), compared with cells transfected with miR-199a-3p mimic negative control or untransfected cells. d mTOR in U87 and U251 with miR-199a-3p over-expression compared with miR-199a-3p mimic negative control (asterisks, P < 0.01). e Cell cycle modulation was restrained resulting in G1 phase increase (asterisks, P < 0.01) and S phase decrease (asterisk, P < 0.05)
Mentions: To gain further insight into the expression of miR-199a-3p in glioma samples and normal brain tissues, quantitative polymerase chain reaction (qPCR) was conducted in 6 normal brain tissues and 61 glioma samples. Results showed that the expression of miR-199a-3p was lower in glioma samples than normal brain tissues (P < 0.01; Fig. 1a), and there was no significant difference between low-grade glioma (grade II) and high-grade (grade III, grade IV) glioma (P > 0.05; Fig. 1a). The expression of mTOR in glioma samples was higher combined with normal brain tissues and increased with the increasing degree of malignancy in glioma (low-grade glioma vs. high-grade glioma, P < 0.01; Fig. 1b). In addition, RhoA and p70S6k expressions in glioma samples were higher than normal brain tissues (P < 0.01; Fig. 1c, d) and had no significant association in low-grade and high-grade glioma samples (P > 0.05; Fig. 1c, d). The expression of miR-199a-3p in U87 and U251 cell lines was very low (P < 0.01; Fig. 2a).Fig. 1

Bottom Line: The over-expression of miR-199a-3p might target mTOR and restrained cellular growth and proliferation but not invasive and apoptosis capability.Results indicated that cellular proliferation was inhibited to regulate the AKT/mTOR signaling pathway by elevating levels of miR-199a-3p.MiR-199a-3p in glioma cell lines has effects similar to the tumor suppressor gene on cellular proliferation via the AKT/mTOR signaling pathway.

View Article: PubMed Central - PubMed

Affiliation: Neurosurgery and Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, Jiangsu, 215006, People's Republic of China.

ABSTRACT
Glioma has been investigated for decades, but the prognosis remains poor because of rapid proliferation, its aggressive potential, and its resistance to chemotherapy or radiotherapy. The mammalian target of rapamycin (mTOR) is highly expressed and regulates cellular proliferation and cell growth. MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene transcription and translation via up-regulating or down-regulating the levels of miRNAs. This study was conducted to explore the molecular functions of miR-199a-3p in glioma. We detected the expression of miR-199a-3p in glioma samples by quantitative PCR (qPCR). Then, we transfected the U87 and U251 cell lines with miR-199a-3p. Cellular proliferation, invasion, and apoptosis were assessed to explain the function of miR-199a-3p. PCR confirmed that the expression of miR-199a-3p was lower in glioma samples combined with normal brain tissues. The over-expression of miR-199a-3p might target mTOR and restrained cellular growth and proliferation but not invasive and apoptosis capability. Results indicated that cellular proliferation was inhibited to regulate the AKT/mTOR signaling pathway by elevating levels of miR-199a-3p. MiR-199a-3p in glioma cell lines has effects similar to the tumor suppressor gene on cellular proliferation via the AKT/mTOR signaling pathway.

Show MeSH
Related in: MedlinePlus