Limits...
An Analog Filter Approach to Frequency Domain Fluorescence Spectroscopy.

Trainham R, O'Neill M, McKenna IJ - J Fluoresc (2015)

Bottom Line: The rate equations found in frequency domain fluorescence spectroscopy are the same as those found in electronics under analog filter theory.The techniques described here can be used to separate signals from fast and slow fluorophores emitting into the same spectral band, and data collection can be greatly accelerated by means of a frequency comb driver waveform and appropriate signal processing of the response.The simplification of the analysis mathematics, and the ability to model the entire detection chain, make it possible to develop more compact instruments for remote sensing applications.

View Article: PubMed Central - PubMed

Affiliation: Special Technologies Laboratory, National Security Technologies, LLC, 5520 Ekwill Street, Santa Barbara, CA, 93111, USA. trainhcp@nv.doe.gov.

ABSTRACT
The rate equations found in frequency domain fluorescence spectroscopy are the same as those found in electronics under analog filter theory. Laplace transform methods are a natural way to solve the equations, and the methods can provide solutions for arbitrary excitation functions. The fluorescence terms can be modelled as circuit components and cascaded with drive and detection electronics to produce a global transfer function. Electronics design tools such as SPICE can be used to model fluorescence problems. In applications, such as remote sensing, where detection electronics are operated at high gain and limited bandwidth, a global modelling of the entire system is important, since the filter terms of the drive and detection electronics affect the measured response of the fluorescence signals. The techniques described here can be used to separate signals from fast and slow fluorophores emitting into the same spectral band, and data collection can be greatly accelerated by means of a frequency comb driver waveform and appropriate signal processing of the response. The simplification of the analysis mathematics, and the ability to model the entire detection chain, make it possible to develop more compact instruments for remote sensing applications.

No MeSH data available.


Related in: MedlinePlus

The modelled phase of the fluorescence response is reproduced using only three frequency settings for the driver excitation: ω = 0.2, ω = 1, and ω = 9, where ω is in units of the fluorescence decay rate Γ
© Copyright Policy - OpenAccess
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4644193&req=5

Fig8: The modelled phase of the fluorescence response is reproduced using only three frequency settings for the driver excitation: ω = 0.2, ω = 1, and ω = 9, where ω is in units of the fluorescence decay rate Γ

Mentions: The harmonic content of a square wave can be exploited to sample the fluorescence response over an extended frequency range using only a few frequency steps of the driving term. In Fig. 8 the modelled phase response has been reproduced with only three frequency settings for the driver: ω = 0.2, ω = 1, and ω = 9, where ω is in units of the fluorescence decay rate Γ.Fig. 8


An Analog Filter Approach to Frequency Domain Fluorescence Spectroscopy.

Trainham R, O'Neill M, McKenna IJ - J Fluoresc (2015)

The modelled phase of the fluorescence response is reproduced using only three frequency settings for the driver excitation: ω = 0.2, ω = 1, and ω = 9, where ω is in units of the fluorescence decay rate Γ
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4644193&req=5

Fig8: The modelled phase of the fluorescence response is reproduced using only three frequency settings for the driver excitation: ω = 0.2, ω = 1, and ω = 9, where ω is in units of the fluorescence decay rate Γ
Mentions: The harmonic content of a square wave can be exploited to sample the fluorescence response over an extended frequency range using only a few frequency steps of the driving term. In Fig. 8 the modelled phase response has been reproduced with only three frequency settings for the driver: ω = 0.2, ω = 1, and ω = 9, where ω is in units of the fluorescence decay rate Γ.Fig. 8

Bottom Line: The rate equations found in frequency domain fluorescence spectroscopy are the same as those found in electronics under analog filter theory.The techniques described here can be used to separate signals from fast and slow fluorophores emitting into the same spectral band, and data collection can be greatly accelerated by means of a frequency comb driver waveform and appropriate signal processing of the response.The simplification of the analysis mathematics, and the ability to model the entire detection chain, make it possible to develop more compact instruments for remote sensing applications.

View Article: PubMed Central - PubMed

Affiliation: Special Technologies Laboratory, National Security Technologies, LLC, 5520 Ekwill Street, Santa Barbara, CA, 93111, USA. trainhcp@nv.doe.gov.

ABSTRACT
The rate equations found in frequency domain fluorescence spectroscopy are the same as those found in electronics under analog filter theory. Laplace transform methods are a natural way to solve the equations, and the methods can provide solutions for arbitrary excitation functions. The fluorescence terms can be modelled as circuit components and cascaded with drive and detection electronics to produce a global transfer function. Electronics design tools such as SPICE can be used to model fluorescence problems. In applications, such as remote sensing, where detection electronics are operated at high gain and limited bandwidth, a global modelling of the entire system is important, since the filter terms of the drive and detection electronics affect the measured response of the fluorescence signals. The techniques described here can be used to separate signals from fast and slow fluorophores emitting into the same spectral band, and data collection can be greatly accelerated by means of a frequency comb driver waveform and appropriate signal processing of the response. The simplification of the analysis mathematics, and the ability to model the entire detection chain, make it possible to develop more compact instruments for remote sensing applications.

No MeSH data available.


Related in: MedlinePlus