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Pulmonary toxicity of well-dispersed cerium oxide nanoparticles following intratracheal instillation and inhalation.

Morimoto Y, Izumi H, Yoshiura Y, Tomonaga T, Oyabu T, Myojo T, Kawai K, Yatera K, Shimada M, Kubo M, Yamamoto K, Kitajima S, Kuroda E, Kawaguchi K, Sasaki T - J Nanopart Res (2015)

Bottom Line: The intratracheal instillation of CeO2 nanoparticles caused a persistent increase in the total and neutrophil number in BALF and in the concentration of cytokine-induced neutrophil chemoattractant (CINC)-1, CINC-2, chemokine for neutrophil, and heme oxygenase-1 (HO-1), an oxidative stress marker, in BALF during the observation time.The inhalation of CeO2 nanoparticles also induced a persistent influx of neutrophils and expression of CINC-1, CINC-2, and HO-1 in BALF.Pathological features revealed that inflammatory cells, including macrophages and neutrophils, invaded the alveolar space in both studies.

View Article: PubMed Central - PubMed

Affiliation: University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahata-nishi-ku, Kitakyushu, Fukuoka 807-8555 Japan.

ABSTRACT

We performed inhalation and intratracheal instillation studies of cerium dioxide (CeO2) nanoparticles in order to investigate their pulmonary toxicity, and observed pulmonary inflammation not only in the acute and but also in the chronic phases. In the intratracheal instillation study, F344 rats were exposed to 0.2 mg or 1 mg of CeO2 nanoparticles. Cell analysis and chemokines in bronchoalveolar lavage fluid (BALF) were analyzed from 3 days to 6 months following the instillation. In the inhalation study, rats were exposed to the maximum concentration of inhaled CeO2 nanoparticles (2, 10 mg/m(3), respectively) for 4 weeks (6 h/day, 5 days/week). The same endpoints as in the intratracheal instillation study were examined from 3 days to 3 months after the end of the exposure. The intratracheal instillation of CeO2 nanoparticles caused a persistent increase in the total and neutrophil number in BALF and in the concentration of cytokine-induced neutrophil chemoattractant (CINC)-1, CINC-2, chemokine for neutrophil, and heme oxygenase-1 (HO-1), an oxidative stress marker, in BALF during the observation time. The inhalation of CeO2 nanoparticles also induced a persistent influx of neutrophils and expression of CINC-1, CINC-2, and HO-1 in BALF. Pathological features revealed that inflammatory cells, including macrophages and neutrophils, invaded the alveolar space in both studies. Taken together, the CeO2 nanoparticles induced not only acute but also chronic inflammation in the lung, suggesting that CeO2 nanoparticles have a pulmonary toxicity that can lead to irreversible lesions.

No MeSH data available.


Related in: MedlinePlus

TEM images of lung tissue in the high-dose group at 3 days (a), and at 3 months (b) after CeO2 inhalation, respectively. Many phagosomes containing CeO2 nanoparticles were observed in alveolar macrophages. The amount of CeO2 nanoparticles in alveolar macrophages decreased according to time
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Fig8: TEM images of lung tissue in the high-dose group at 3 days (a), and at 3 months (b) after CeO2 inhalation, respectively. Many phagosomes containing CeO2 nanoparticles were observed in alveolar macrophages. The amount of CeO2 nanoparticles in alveolar macrophages decreased according to time

Mentions: Compared to the inhalation groups, the macrophages in the instillation groups were accompanied by more lymphocytic infiltrations. Changes in the number of intracytoplasmic particles were different between the instillation groups and the inhalation groups in a time course, with more particles being observed in the early periods in the instillation groups, and a higher number of particles in the late periods in the inhalation groups.


Pulmonary toxicity of well-dispersed cerium oxide nanoparticles following intratracheal instillation and inhalation.

Morimoto Y, Izumi H, Yoshiura Y, Tomonaga T, Oyabu T, Myojo T, Kawai K, Yatera K, Shimada M, Kubo M, Yamamoto K, Kitajima S, Kuroda E, Kawaguchi K, Sasaki T - J Nanopart Res (2015)

TEM images of lung tissue in the high-dose group at 3 days (a), and at 3 months (b) after CeO2 inhalation, respectively. Many phagosomes containing CeO2 nanoparticles were observed in alveolar macrophages. The amount of CeO2 nanoparticles in alveolar macrophages decreased according to time
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4644191&req=5

Fig8: TEM images of lung tissue in the high-dose group at 3 days (a), and at 3 months (b) after CeO2 inhalation, respectively. Many phagosomes containing CeO2 nanoparticles were observed in alveolar macrophages. The amount of CeO2 nanoparticles in alveolar macrophages decreased according to time
Mentions: Compared to the inhalation groups, the macrophages in the instillation groups were accompanied by more lymphocytic infiltrations. Changes in the number of intracytoplasmic particles were different between the instillation groups and the inhalation groups in a time course, with more particles being observed in the early periods in the instillation groups, and a higher number of particles in the late periods in the inhalation groups.

Bottom Line: The intratracheal instillation of CeO2 nanoparticles caused a persistent increase in the total and neutrophil number in BALF and in the concentration of cytokine-induced neutrophil chemoattractant (CINC)-1, CINC-2, chemokine for neutrophil, and heme oxygenase-1 (HO-1), an oxidative stress marker, in BALF during the observation time.The inhalation of CeO2 nanoparticles also induced a persistent influx of neutrophils and expression of CINC-1, CINC-2, and HO-1 in BALF.Pathological features revealed that inflammatory cells, including macrophages and neutrophils, invaded the alveolar space in both studies.

View Article: PubMed Central - PubMed

Affiliation: University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahata-nishi-ku, Kitakyushu, Fukuoka 807-8555 Japan.

ABSTRACT

We performed inhalation and intratracheal instillation studies of cerium dioxide (CeO2) nanoparticles in order to investigate their pulmonary toxicity, and observed pulmonary inflammation not only in the acute and but also in the chronic phases. In the intratracheal instillation study, F344 rats were exposed to 0.2 mg or 1 mg of CeO2 nanoparticles. Cell analysis and chemokines in bronchoalveolar lavage fluid (BALF) were analyzed from 3 days to 6 months following the instillation. In the inhalation study, rats were exposed to the maximum concentration of inhaled CeO2 nanoparticles (2, 10 mg/m(3), respectively) for 4 weeks (6 h/day, 5 days/week). The same endpoints as in the intratracheal instillation study were examined from 3 days to 3 months after the end of the exposure. The intratracheal instillation of CeO2 nanoparticles caused a persistent increase in the total and neutrophil number in BALF and in the concentration of cytokine-induced neutrophil chemoattractant (CINC)-1, CINC-2, chemokine for neutrophil, and heme oxygenase-1 (HO-1), an oxidative stress marker, in BALF during the observation time. The inhalation of CeO2 nanoparticles also induced a persistent influx of neutrophils and expression of CINC-1, CINC-2, and HO-1 in BALF. Pathological features revealed that inflammatory cells, including macrophages and neutrophils, invaded the alveolar space in both studies. Taken together, the CeO2 nanoparticles induced not only acute but also chronic inflammation in the lung, suggesting that CeO2 nanoparticles have a pulmonary toxicity that can lead to irreversible lesions.

No MeSH data available.


Related in: MedlinePlus