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Engineering iodine-doped carbon dots as dual-modal probes for fluorescence and X-ray CT imaging.

Zhang M, Ju H, Zhang L, Sun M, Zhou Z, Dai Z, Zhang L, Gong A, Wu C, Du F - Int J Nanomedicine (2015)

Bottom Line: Importantly, I-doped CDs displayed superior X-ray attenuation properties in vitro and excellent biocompatibility.After intravenous injection, I-doped CDs were distributed throughout the body and excreted by renal clearance.These findings validated that I-doped CDs with high X-ray attenuation potency and favorable photoluminescence show great promise for biomedical research and disease diagnosis.

View Article: PubMed Central - PubMed

Affiliation: School of Medicine, Jiangsu University, Zhenjiang, People's Republic of China.

ABSTRACT
X-ray computed tomography (CT) is the most commonly used imaging technique for noninvasive diagnosis of disease. In order to improve tissue specificity and prevent adverse effects, we report the design and synthesis of iodine-doped carbon dots (I-doped CDs) as efficient CT contrast agents and fluorescence probe by a facile bottom-up hydrothermal carbonization process. The as-prepared I-doped CDs are monodispersed spherical nanoparticles (a diameter of ~2.7 nm) with favorable dispersibility and colloidal stability in water. The aqueous solution of I-doped CDs showed wavelength-dependent excitation and stable photoluminescence similar to traditional carbon quantum dots. Importantly, I-doped CDs displayed superior X-ray attenuation properties in vitro and excellent biocompatibility. After intravenous injection, I-doped CDs were distributed throughout the body and excreted by renal clearance. These findings validated that I-doped CDs with high X-ray attenuation potency and favorable photoluminescence show great promise for biomedical research and disease diagnosis.

No MeSH data available.


Histological changes in the organs of the rats 7 days after intravenous injection of I-doped CDs at the dose of 10 mg/kg.Notes: These organs were stained with H&E and observed under a light microscope (scale bars =50 μm). The inset figures represent the pictures were observed under high resolution.Abbreviations: I-doped CDs, iodine-doped carbon dots; H&E, hematoxylin and eosin.
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f8-ijn-10-6943: Histological changes in the organs of the rats 7 days after intravenous injection of I-doped CDs at the dose of 10 mg/kg.Notes: These organs were stained with H&E and observed under a light microscope (scale bars =50 μm). The inset figures represent the pictures were observed under high resolution.Abbreviations: I-doped CDs, iodine-doped carbon dots; H&E, hematoxylin and eosin.

Mentions: The possibility to use I-doped CDs as contrast agents for CT imaging in vivo was studied using Sprague Dawley rats as a model. After intravenous injection of I-doped CDs at a dose of 40 mg/kg, the CT images were acquired through different scans. Compared with preinjection (Figure 7A), a great contrast enhancement in bladder was observed clearly using the three dimensional (3-D) CT imaging (as shown in Figure 7B). Figure 7C showed that the signal both in the kidney and bladder strongly increased at 10 minutes postinjection, which indicated I-doped CDs accumulation. These results were further confirmed through CT transverse scans at different levels (Figure 7D and E). To further investigate whether I-doped CDs can induce adverse effect (such as tissue damage, inflammation, or lesions) during the long-term postinjection, we conducted the histological assessment of the mouse susceptible organs (liver, heart, spleen, lung, and kidney). As shown in Figure 8, no obvious adverse effect associated with the administration of the I-doped CDs was observed compared with the control group. Based on the results described above, we proposed that the I-doped CDs were rapidly distributed throughout the body and excreted from the bladder.


Engineering iodine-doped carbon dots as dual-modal probes for fluorescence and X-ray CT imaging.

Zhang M, Ju H, Zhang L, Sun M, Zhou Z, Dai Z, Zhang L, Gong A, Wu C, Du F - Int J Nanomedicine (2015)

Histological changes in the organs of the rats 7 days after intravenous injection of I-doped CDs at the dose of 10 mg/kg.Notes: These organs were stained with H&E and observed under a light microscope (scale bars =50 μm). The inset figures represent the pictures were observed under high resolution.Abbreviations: I-doped CDs, iodine-doped carbon dots; H&E, hematoxylin and eosin.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4644166&req=5

f8-ijn-10-6943: Histological changes in the organs of the rats 7 days after intravenous injection of I-doped CDs at the dose of 10 mg/kg.Notes: These organs were stained with H&E and observed under a light microscope (scale bars =50 μm). The inset figures represent the pictures were observed under high resolution.Abbreviations: I-doped CDs, iodine-doped carbon dots; H&E, hematoxylin and eosin.
Mentions: The possibility to use I-doped CDs as contrast agents for CT imaging in vivo was studied using Sprague Dawley rats as a model. After intravenous injection of I-doped CDs at a dose of 40 mg/kg, the CT images were acquired through different scans. Compared with preinjection (Figure 7A), a great contrast enhancement in bladder was observed clearly using the three dimensional (3-D) CT imaging (as shown in Figure 7B). Figure 7C showed that the signal both in the kidney and bladder strongly increased at 10 minutes postinjection, which indicated I-doped CDs accumulation. These results were further confirmed through CT transverse scans at different levels (Figure 7D and E). To further investigate whether I-doped CDs can induce adverse effect (such as tissue damage, inflammation, or lesions) during the long-term postinjection, we conducted the histological assessment of the mouse susceptible organs (liver, heart, spleen, lung, and kidney). As shown in Figure 8, no obvious adverse effect associated with the administration of the I-doped CDs was observed compared with the control group. Based on the results described above, we proposed that the I-doped CDs were rapidly distributed throughout the body and excreted from the bladder.

Bottom Line: Importantly, I-doped CDs displayed superior X-ray attenuation properties in vitro and excellent biocompatibility.After intravenous injection, I-doped CDs were distributed throughout the body and excreted by renal clearance.These findings validated that I-doped CDs with high X-ray attenuation potency and favorable photoluminescence show great promise for biomedical research and disease diagnosis.

View Article: PubMed Central - PubMed

Affiliation: School of Medicine, Jiangsu University, Zhenjiang, People's Republic of China.

ABSTRACT
X-ray computed tomography (CT) is the most commonly used imaging technique for noninvasive diagnosis of disease. In order to improve tissue specificity and prevent adverse effects, we report the design and synthesis of iodine-doped carbon dots (I-doped CDs) as efficient CT contrast agents and fluorescence probe by a facile bottom-up hydrothermal carbonization process. The as-prepared I-doped CDs are monodispersed spherical nanoparticles (a diameter of ~2.7 nm) with favorable dispersibility and colloidal stability in water. The aqueous solution of I-doped CDs showed wavelength-dependent excitation and stable photoluminescence similar to traditional carbon quantum dots. Importantly, I-doped CDs displayed superior X-ray attenuation properties in vitro and excellent biocompatibility. After intravenous injection, I-doped CDs were distributed throughout the body and excreted by renal clearance. These findings validated that I-doped CDs with high X-ray attenuation potency and favorable photoluminescence show great promise for biomedical research and disease diagnosis.

No MeSH data available.