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The IMSI Procedure Improves Laboratory and Clinical Outcomes Without Compromising the Aneuploidy Rate When Compared to the Classical ICSI Procedure.

Luna D, Hilario R, Dueñas-Chacón J, Romero R, Zavala P, Villegas L, García-Ferreyra J - Clin Med Insights Reprod Health (2015)

Bottom Line: The intracytoplasmic morphologically selected sperm injection (IMSI) procedure has been associated with better laboratory and clinical outcomes in assisted reproduction technologies.The fertilization rate, cleavage rate, embryo quality, blastocyst development, aneuploidy rate, pregnancy rate, implantation rate, and miscarriage rate were compared between the groups.The IMSI procedure significantly improves the embryo quality/development by increasing the implantation rates without affecting the chromosomal status of embryos.

View Article: PubMed Central - PubMed

Affiliation: FERTILAB Laboratory of Assisted Reproduction, Lima, Perú.

ABSTRACT

Purpose: The intracytoplasmic morphologically selected sperm injection (IMSI) procedure has been associated with better laboratory and clinical outcomes in assisted reproduction technologies. Less information is available regarding the relationship between embryo aneuploidy rate and the IMSI procedure. The aim of this study is to compare the clinical outcomes and chromosomal status of IMSI-derived embryos with those obtained from intracytoplasmic sperm injection (ICSI) in order to establish a clearer view of the benefits of IMSI in infertile patients.

Methods: We retrospectively analyzed a total of 11 cycles of IMSI and 20 cycles of ICSI with preimplantation genetic diagnosis. The fertilization rate, cleavage rate, embryo quality, blastocyst development, aneuploidy rate, pregnancy rate, implantation rate, and miscarriage rate were compared between the groups.

Results: Similar rates of fertilization (70% and 73%), cleavage (98% and 100%), and aneuploidy (76.9% and 70.9%) were observed in the IMSI and ICSI groups, respectively. The IMSI group had significantly more good quality embryos at day 3 (95% vs 73%), higher blastocyst development rates (33% vs 19%), and greater number of hatching blastocysts (43% vs 28%), cycles with at least one blastocyst at day 5 (55% vs 35%), and blastocysts with good trophoectoderm morphology (21% vs 6%) compared with the ICSI group (P < 0.001). Significantly higher implantation rates were observed in the IMSI group compared with the ICSI group (57% vs 27%; P < 0.05). Pregnancy and miscarriage rates were similar in both groups (80% vs 50% and 0% vs 33%, respectively).

Conclusion: The IMSI procedure significantly improves the embryo quality/development by increasing the implantation rates without affecting the chromosomal status of embryos. There is a tendency for the IMSI procedure to enhance the pregnancy rates and lower the miscarriage rates when compared with ICSI.

No MeSH data available.


Related in: MedlinePlus

Grading of spermatozoa into four groups according to the presence or size of vacuoles. Grade I: normal form and no vacuoles (A). Grade II: normal form with less than or equal to two small vacuoles (B, C). Grade III: normal form with more than two small vacuoles or at least one large vacuole (D, E). Grade IV: large vacuole and abnormal head shapes or other abnormalities (F, G).
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f1-cmrh-9-2015-029: Grading of spermatozoa into four groups according to the presence or size of vacuoles. Grade I: normal form and no vacuoles (A). Grade II: normal form with less than or equal to two small vacuoles (B, C). Grade III: normal form with more than two small vacuoles or at least one large vacuole (D, E). Grade IV: large vacuole and abnormal head shapes or other abnormalities (F, G).

Mentions: For sperm retrieval and immobilization, a glass bottom dish (Willco Wells) with one 5-μL microdroplet of polyvinylpyrrolidone (PVP; Irvine Scientific) and another microdroplet of Global® HEPES-buffered medium supplemented with 10% vol/vol SSS under mineral oil were prepared. Spermatozoa were selected under ×10,160 magnification in an inverted microscope (IX71; Olympus) equipped with a Normarski differential interference contrast optics, ×100/1.30 oil objective lens, and a variable zoom lens. Spermatozoa were retrieved from the medium and then transferred to the PVP microdroplet to be immobilized by tail crushing. Afterward, they were classified according to their morphology and grade of vacuolization pattern (types I–IV) as described by Vanderzwalmen et al4 (Fig. 1). The spermatozoa with a normal morphology and presenting a vacuolization pattern of type I or II were used for injection into the oocytes.


The IMSI Procedure Improves Laboratory and Clinical Outcomes Without Compromising the Aneuploidy Rate When Compared to the Classical ICSI Procedure.

Luna D, Hilario R, Dueñas-Chacón J, Romero R, Zavala P, Villegas L, García-Ferreyra J - Clin Med Insights Reprod Health (2015)

Grading of spermatozoa into four groups according to the presence or size of vacuoles. Grade I: normal form and no vacuoles (A). Grade II: normal form with less than or equal to two small vacuoles (B, C). Grade III: normal form with more than two small vacuoles or at least one large vacuole (D, E). Grade IV: large vacuole and abnormal head shapes or other abnormalities (F, G).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4644143&req=5

f1-cmrh-9-2015-029: Grading of spermatozoa into four groups according to the presence or size of vacuoles. Grade I: normal form and no vacuoles (A). Grade II: normal form with less than or equal to two small vacuoles (B, C). Grade III: normal form with more than two small vacuoles or at least one large vacuole (D, E). Grade IV: large vacuole and abnormal head shapes or other abnormalities (F, G).
Mentions: For sperm retrieval and immobilization, a glass bottom dish (Willco Wells) with one 5-μL microdroplet of polyvinylpyrrolidone (PVP; Irvine Scientific) and another microdroplet of Global® HEPES-buffered medium supplemented with 10% vol/vol SSS under mineral oil were prepared. Spermatozoa were selected under ×10,160 magnification in an inverted microscope (IX71; Olympus) equipped with a Normarski differential interference contrast optics, ×100/1.30 oil objective lens, and a variable zoom lens. Spermatozoa were retrieved from the medium and then transferred to the PVP microdroplet to be immobilized by tail crushing. Afterward, they were classified according to their morphology and grade of vacuolization pattern (types I–IV) as described by Vanderzwalmen et al4 (Fig. 1). The spermatozoa with a normal morphology and presenting a vacuolization pattern of type I or II were used for injection into the oocytes.

Bottom Line: The intracytoplasmic morphologically selected sperm injection (IMSI) procedure has been associated with better laboratory and clinical outcomes in assisted reproduction technologies.The fertilization rate, cleavage rate, embryo quality, blastocyst development, aneuploidy rate, pregnancy rate, implantation rate, and miscarriage rate were compared between the groups.The IMSI procedure significantly improves the embryo quality/development by increasing the implantation rates without affecting the chromosomal status of embryos.

View Article: PubMed Central - PubMed

Affiliation: FERTILAB Laboratory of Assisted Reproduction, Lima, Perú.

ABSTRACT

Purpose: The intracytoplasmic morphologically selected sperm injection (IMSI) procedure has been associated with better laboratory and clinical outcomes in assisted reproduction technologies. Less information is available regarding the relationship between embryo aneuploidy rate and the IMSI procedure. The aim of this study is to compare the clinical outcomes and chromosomal status of IMSI-derived embryos with those obtained from intracytoplasmic sperm injection (ICSI) in order to establish a clearer view of the benefits of IMSI in infertile patients.

Methods: We retrospectively analyzed a total of 11 cycles of IMSI and 20 cycles of ICSI with preimplantation genetic diagnosis. The fertilization rate, cleavage rate, embryo quality, blastocyst development, aneuploidy rate, pregnancy rate, implantation rate, and miscarriage rate were compared between the groups.

Results: Similar rates of fertilization (70% and 73%), cleavage (98% and 100%), and aneuploidy (76.9% and 70.9%) were observed in the IMSI and ICSI groups, respectively. The IMSI group had significantly more good quality embryos at day 3 (95% vs 73%), higher blastocyst development rates (33% vs 19%), and greater number of hatching blastocysts (43% vs 28%), cycles with at least one blastocyst at day 5 (55% vs 35%), and blastocysts with good trophoectoderm morphology (21% vs 6%) compared with the ICSI group (P < 0.001). Significantly higher implantation rates were observed in the IMSI group compared with the ICSI group (57% vs 27%; P < 0.05). Pregnancy and miscarriage rates were similar in both groups (80% vs 50% and 0% vs 33%, respectively).

Conclusion: The IMSI procedure significantly improves the embryo quality/development by increasing the implantation rates without affecting the chromosomal status of embryos. There is a tendency for the IMSI procedure to enhance the pregnancy rates and lower the miscarriage rates when compared with ICSI.

No MeSH data available.


Related in: MedlinePlus