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Trinitrotoluene Induces Endoplasmic Reticulum Stress and Apoptosis in HePG2 Cells.

Song L, Wang Y, Wang J, Yang F, Li X, Wu Y - Med. Sci. Monit. (2015)

Bottom Line: Real-time PCR was used to measure mRNA expression from the respective genes.In addition, the mRNA and protein expression levels of ERS-induced apoptotic protein Caspase-4 were significantly increased.Flow cytometry revealed that after TNT treatment, the apoptosis rate also significantly increased.

View Article: PubMed Central - PubMed

Affiliation: Department of Occupational and Environmental Health, School of Public Health, Harbin Medical University, Harbin, Heilongjiang, China (mainland).

ABSTRACT

Background: This study aims to describe trinitrotoluene (TNT)-induced endoplasmic reticulum stress (ERS) and apoptosis in HePG2 cells.

Material and methods: HePG2 cells were cultured in vitro with 0, 6, 12, or 24 μg/ml TNT solution for 12, 24, and 48 h. Western blotting was performed to detect intracellular ERS-related proteins, including glucose-regulated protein (GRP) 78, GRP94, Caspase 4, p-Jun N-terminal kinase (JNK), and C/EBP homologous protein (CHOP). Real-time PCR was used to measure mRNA expression from the respective genes.

Results: The expressions of ERS-related proteins GRP78 and GRP94 as well as mRNA and protein expression of ERS signaling apoptotic CHOP in the TNT treatment group were significantly increased. In addition, the mRNA and protein expression levels of ERS-induced apoptotic protein Caspase-4 were significantly increased. Flow cytometry revealed that after TNT treatment, the apoptosis rate also significantly increased.

Conclusions: TNT could increase the expression levels of GRP78, GRP94, Caspase-4, and CHOP in HePG2 cells; this increase in protein expression might be involved in HePG2 apoptosis through the induction of the ERS pathway.

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Expression levels of Caspase-4 protein.
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f4-medscimonit-21-3434: Expression levels of Caspase-4 protein.

Mentions: After HePG2 cells were stimulated with 0, 6, 12, and 24 μg/mL TNT for 24 h, Western blot and RT-PCR were performed to measure C CASP4 expression. TNT exposure significantly increased C CASP4 protein and mRNA expression (P<0.05, Figure 4, Table 4).


Trinitrotoluene Induces Endoplasmic Reticulum Stress and Apoptosis in HePG2 Cells.

Song L, Wang Y, Wang J, Yang F, Li X, Wu Y - Med. Sci. Monit. (2015)

Expression levels of Caspase-4 protein.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4644021&req=5

f4-medscimonit-21-3434: Expression levels of Caspase-4 protein.
Mentions: After HePG2 cells were stimulated with 0, 6, 12, and 24 μg/mL TNT for 24 h, Western blot and RT-PCR were performed to measure C CASP4 expression. TNT exposure significantly increased C CASP4 protein and mRNA expression (P<0.05, Figure 4, Table 4).

Bottom Line: Real-time PCR was used to measure mRNA expression from the respective genes.In addition, the mRNA and protein expression levels of ERS-induced apoptotic protein Caspase-4 were significantly increased.Flow cytometry revealed that after TNT treatment, the apoptosis rate also significantly increased.

View Article: PubMed Central - PubMed

Affiliation: Department of Occupational and Environmental Health, School of Public Health, Harbin Medical University, Harbin, Heilongjiang, China (mainland).

ABSTRACT

Background: This study aims to describe trinitrotoluene (TNT)-induced endoplasmic reticulum stress (ERS) and apoptosis in HePG2 cells.

Material and methods: HePG2 cells were cultured in vitro with 0, 6, 12, or 24 μg/ml TNT solution for 12, 24, and 48 h. Western blotting was performed to detect intracellular ERS-related proteins, including glucose-regulated protein (GRP) 78, GRP94, Caspase 4, p-Jun N-terminal kinase (JNK), and C/EBP homologous protein (CHOP). Real-time PCR was used to measure mRNA expression from the respective genes.

Results: The expressions of ERS-related proteins GRP78 and GRP94 as well as mRNA and protein expression of ERS signaling apoptotic CHOP in the TNT treatment group were significantly increased. In addition, the mRNA and protein expression levels of ERS-induced apoptotic protein Caspase-4 were significantly increased. Flow cytometry revealed that after TNT treatment, the apoptosis rate also significantly increased.

Conclusions: TNT could increase the expression levels of GRP78, GRP94, Caspase-4, and CHOP in HePG2 cells; this increase in protein expression might be involved in HePG2 apoptosis through the induction of the ERS pathway.

Show MeSH