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LPS-Stimulated Human Skin-Derived Stem Cells Enhance Neo-Vascularization during Dermal Regeneration.

Kisch T, Weber C, Rapoport DH, Kruse C, Schumann S, Stang FH, Siemers F, Matthießen AE - PLoS ONE (2015)

Bottom Line: High numbers of adult stem cells are still required to improve the formation of new vessels in scaffolds to accelerate dermal regeneration.Results showed that LPS-activated SDSC significantly enhanced vascularization of the scaffolds, compared to unstimulated stem cells in vivo.Our results suggest that combining activated stem cells and a dermal substitute is a promising option to enhance vascularization in scaffold-mediated dermal regeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic Surgery and Hand Surgery, University of Lübeck, Lübeck, Germany.

ABSTRACT
High numbers of adult stem cells are still required to improve the formation of new vessels in scaffolds to accelerate dermal regeneration. Recent data indicate a benefit for vascularization capacity by stimulating stem cells with lipopolysaccharide (LPS). In this study, stem cells derived from human skin (SDSC) were activated with LPS and seeded in a commercially available dermal substitute to examine vascularization in vivo. Besides, in vitro assays were performed to evaluate angiogenic factor release and tube formation ability. Results showed that LPS-activated SDSC significantly enhanced vascularization of the scaffolds, compared to unstimulated stem cells in vivo. Further, in vitro assays confirmed higher secretion rates of proangiogenic as well as proinflammatoric factors in the presence of LPS-activated SDSC. Our results suggest that combining activated stem cells and a dermal substitute is a promising option to enhance vascularization in scaffold-mediated dermal regeneration.

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Related in: MedlinePlus

LPS Uptake.An immunocytochemical staining revealed the expression of TLR4 in skin-derived stem cells (SDSC). The incorporation of LPS could be shown by staining of LPS in SDSC. The LPS uptake led to a significantly enhanced expression of TLR4 mRNA 4 hours after stimulation with 10ng/ml LPS. Afterwards TLR4 expression returned to normal level. *p<0.05.
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pone.0142907.g001: LPS Uptake.An immunocytochemical staining revealed the expression of TLR4 in skin-derived stem cells (SDSC). The incorporation of LPS could be shown by staining of LPS in SDSC. The LPS uptake led to a significantly enhanced expression of TLR4 mRNA 4 hours after stimulation with 10ng/ml LPS. Afterwards TLR4 expression returned to normal level. *p<0.05.

Mentions: LPS is known to be recognized via the toll-like receptor 4 (TLR-4)/MD2 receptor complex and binding to the co-receptor CD14. An immunocytochemical staining revealed the expression of TLR-4 on the surface of human skin-derived stem cells (SDSC), after cells had been successfully isolated from human full skin biopsies. The addition of LPS, obtained from E.coli 11:B4, to the growth medium led to an incorporation of LPS into the cells, as confirmed by immunocytochemical staining. Subsequent intracellular uptake of LPS resulted in significantly enhanced expression of TLR-4 mRNA within 4 h. At later time points (24 h or 48 h after stimulation) TLR-4 mRNA expression had again decreased to baseline levels (Fig 1).


LPS-Stimulated Human Skin-Derived Stem Cells Enhance Neo-Vascularization during Dermal Regeneration.

Kisch T, Weber C, Rapoport DH, Kruse C, Schumann S, Stang FH, Siemers F, Matthießen AE - PLoS ONE (2015)

LPS Uptake.An immunocytochemical staining revealed the expression of TLR4 in skin-derived stem cells (SDSC). The incorporation of LPS could be shown by staining of LPS in SDSC. The LPS uptake led to a significantly enhanced expression of TLR4 mRNA 4 hours after stimulation with 10ng/ml LPS. Afterwards TLR4 expression returned to normal level. *p<0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4643997&req=5

pone.0142907.g001: LPS Uptake.An immunocytochemical staining revealed the expression of TLR4 in skin-derived stem cells (SDSC). The incorporation of LPS could be shown by staining of LPS in SDSC. The LPS uptake led to a significantly enhanced expression of TLR4 mRNA 4 hours after stimulation with 10ng/ml LPS. Afterwards TLR4 expression returned to normal level. *p<0.05.
Mentions: LPS is known to be recognized via the toll-like receptor 4 (TLR-4)/MD2 receptor complex and binding to the co-receptor CD14. An immunocytochemical staining revealed the expression of TLR-4 on the surface of human skin-derived stem cells (SDSC), after cells had been successfully isolated from human full skin biopsies. The addition of LPS, obtained from E.coli 11:B4, to the growth medium led to an incorporation of LPS into the cells, as confirmed by immunocytochemical staining. Subsequent intracellular uptake of LPS resulted in significantly enhanced expression of TLR-4 mRNA within 4 h. At later time points (24 h or 48 h after stimulation) TLR-4 mRNA expression had again decreased to baseline levels (Fig 1).

Bottom Line: High numbers of adult stem cells are still required to improve the formation of new vessels in scaffolds to accelerate dermal regeneration.Results showed that LPS-activated SDSC significantly enhanced vascularization of the scaffolds, compared to unstimulated stem cells in vivo.Our results suggest that combining activated stem cells and a dermal substitute is a promising option to enhance vascularization in scaffold-mediated dermal regeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic Surgery and Hand Surgery, University of Lübeck, Lübeck, Germany.

ABSTRACT
High numbers of adult stem cells are still required to improve the formation of new vessels in scaffolds to accelerate dermal regeneration. Recent data indicate a benefit for vascularization capacity by stimulating stem cells with lipopolysaccharide (LPS). In this study, stem cells derived from human skin (SDSC) were activated with LPS and seeded in a commercially available dermal substitute to examine vascularization in vivo. Besides, in vitro assays were performed to evaluate angiogenic factor release and tube formation ability. Results showed that LPS-activated SDSC significantly enhanced vascularization of the scaffolds, compared to unstimulated stem cells in vivo. Further, in vitro assays confirmed higher secretion rates of proangiogenic as well as proinflammatoric factors in the presence of LPS-activated SDSC. Our results suggest that combining activated stem cells and a dermal substitute is a promising option to enhance vascularization in scaffold-mediated dermal regeneration.

Show MeSH
Related in: MedlinePlus