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Lily Cultivars Have Allelopathic Potential in Controlling Orobanche aegyptiaca Persoon.

Chai M, Zhu X, Cui H, Jiang C, Zhang J, Shi L - PLoS ONE (2015)

Bottom Line: Specifically, Sorbonne and Ceb Dazzle behaved with similar allelopathy, and the bulb, scale leaf and aerial stem exhibited stronger allelopathic effects on O. aegyptiaca Pers. germination compared to other organs.Aqueous L. formolongo leaf extracts may contain more stable, effective stimulants given that they induced the highest germination rate at 76.7% even though the extracts were serially diluted.We speculate that these organs may be advantageous in further isolating and purifying economical active substances that can be substitutes for GR24.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.

ABSTRACT
As a devastating holoparasitic weed, Orobanche aegyptiaca Persoon. (Egyptian broomrape) causes serious damage to agricultural production and threatens economic development, which has raised widespread concern. The present study was conducted to determine whether lilies have the potential to be used as 'trap crops' for controlling O. aegyptiaca Persoon. In the experiments, the ability of three popular lily cultivars (Lilium Oriental hybrids 'Sorbonne', Lilium LA (Longiflorum hybrids x Asiatic hybrids) hybrids 'Ceb Dazzle', and Lilium Longiflorum hybrids (L. formosanum x L. longiflorum) 'L. formolongo') to induce O. aegyptiaca Persoon. seed germination was assessed. Parts of the three lily cultivars, including the rhizosphere soil and underground and above-ground organs, all induced "suicidal germination" of parasitic O. aegyptiaca Persoon. seed at four growth stages. Specifically, Sorbonne and Ceb Dazzle behaved with similar allelopathy, and the bulb, scale leaf and aerial stem exhibited stronger allelopathic effects on O. aegyptiaca Pers. germination compared to other organs. Aqueous L. formolongo leaf extracts may contain more stable, effective stimulants given that they induced the highest germination rate at 76.7% even though the extracts were serially diluted. We speculate that these organs may be advantageous in further isolating and purifying economical active substances that can be substitutes for GR24. These results indicate that lilies have the potential to be used as a trap crops or can be processed into green herbicide formulations that can be applied in agriculture production to rapidly deplete the seed bank of O. aegyptiaca Persoon. parasitic weeds in soil.

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Related in: MedlinePlus

Induction of O. aegyptiaca Pers. germination by aqueous and methanol extracts of lily underground (A) and above-ground (B) organs at the leaf expansion stage.Two types of organs were tested, (A) underground organs and (B) above-ground organs. Two types of extracts were tested, (A1 and B1) aqueous and (A2 and B2) methanol. Four concentrations of extracts were assessed: undiluted and 10-fold, 100-fold and 1000-fold dilutions. Abbreviations: R-Sor, root extracts of Sorbonne; R-Ceb, root extracts of Ceb Dazzle; R-Lor, root extracts of L. formolongo; B-Sor, bulb extracts of Sorbonne; B-Ceb, bulb extracts of Ceb Dazzle; B-Lor, bulb extracts of L. formolongo; S-Sor, scale leaf extracts of Sorbonne; S-Ceb, scale leaf extracts of Ceb Dazzle; S-Lor, scale leaf extracts of L. formolongo; P-Sor, prop root extracts of Sorbonne; P-Ceb, prop root extracts of Ceb Dazzle; P-Lor, prop root extracts of L.formolongo; A-Sor, aerial stem extracts of Sorbonne; A-Ceb, aerial stem extracts of Ceb Dazzle; A-Lor, aerial stem extracts of L.formolongo.; L-Sor, leaf extracts of Sorbonne; L-Ceb, leaf extracts of Ceb Dazzle; L-Lor, leaf extracts of L.formolongo; CK1, GR24 as positive control; CK2, Distilled water as negative control; CK3, Methanol as negative control. The dotted black lines represent 20% and 40% position, respectively. Error bars represent the standard error of the mean. Different small letters above the error bars indicate significant differences at 0.05 (ANOVA and Duncan's multiple range test). Matching letters in the same color column indicates that there were no significant differences between treatments.
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pone.0142811.g003: Induction of O. aegyptiaca Pers. germination by aqueous and methanol extracts of lily underground (A) and above-ground (B) organs at the leaf expansion stage.Two types of organs were tested, (A) underground organs and (B) above-ground organs. Two types of extracts were tested, (A1 and B1) aqueous and (A2 and B2) methanol. Four concentrations of extracts were assessed: undiluted and 10-fold, 100-fold and 1000-fold dilutions. Abbreviations: R-Sor, root extracts of Sorbonne; R-Ceb, root extracts of Ceb Dazzle; R-Lor, root extracts of L. formolongo; B-Sor, bulb extracts of Sorbonne; B-Ceb, bulb extracts of Ceb Dazzle; B-Lor, bulb extracts of L. formolongo; S-Sor, scale leaf extracts of Sorbonne; S-Ceb, scale leaf extracts of Ceb Dazzle; S-Lor, scale leaf extracts of L. formolongo; P-Sor, prop root extracts of Sorbonne; P-Ceb, prop root extracts of Ceb Dazzle; P-Lor, prop root extracts of L.formolongo; A-Sor, aerial stem extracts of Sorbonne; A-Ceb, aerial stem extracts of Ceb Dazzle; A-Lor, aerial stem extracts of L.formolongo.; L-Sor, leaf extracts of Sorbonne; L-Ceb, leaf extracts of Ceb Dazzle; L-Lor, leaf extracts of L.formolongo; CK1, GR24 as positive control; CK2, Distilled water as negative control; CK3, Methanol as negative control. The dotted black lines represent 20% and 40% position, respectively. Error bars represent the standard error of the mean. Different small letters above the error bars indicate significant differences at 0.05 (ANOVA and Duncan's multiple range test). Matching letters in the same color column indicates that there were no significant differences between treatments.

Mentions: The highest germination rates were induced by 10-fold dilution of aqueous and methanol extracts from underground organs (Fig 3). Maximum germination rates induced by 10-fold dilution of both aqueous and methanol extracts by organ were as follows: bulb > scale leaf > root in both Ceb Dazzle and L. formolongo. Differences between organs was significant (P<0.05) (Fig 4) (S2 Table). Ceb Dazzle (45.1%) and L. formolongo (60.1%) bulbs had relatively stable, strong allelopathic effects on O. aegyptiaca Pers. seeds, which were similar to effects in the sprouting stage (Figs 1 and 4). Although there were no significant differences between the underground organs of the Sorbonne cultivar, the highest germination rate was induced by scale leaf (50.1%), which was increased beyond the rate induced by bulbs in the leaf expansion stage (Fig 3A2).


Lily Cultivars Have Allelopathic Potential in Controlling Orobanche aegyptiaca Persoon.

Chai M, Zhu X, Cui H, Jiang C, Zhang J, Shi L - PLoS ONE (2015)

Induction of O. aegyptiaca Pers. germination by aqueous and methanol extracts of lily underground (A) and above-ground (B) organs at the leaf expansion stage.Two types of organs were tested, (A) underground organs and (B) above-ground organs. Two types of extracts were tested, (A1 and B1) aqueous and (A2 and B2) methanol. Four concentrations of extracts were assessed: undiluted and 10-fold, 100-fold and 1000-fold dilutions. Abbreviations: R-Sor, root extracts of Sorbonne; R-Ceb, root extracts of Ceb Dazzle; R-Lor, root extracts of L. formolongo; B-Sor, bulb extracts of Sorbonne; B-Ceb, bulb extracts of Ceb Dazzle; B-Lor, bulb extracts of L. formolongo; S-Sor, scale leaf extracts of Sorbonne; S-Ceb, scale leaf extracts of Ceb Dazzle; S-Lor, scale leaf extracts of L. formolongo; P-Sor, prop root extracts of Sorbonne; P-Ceb, prop root extracts of Ceb Dazzle; P-Lor, prop root extracts of L.formolongo; A-Sor, aerial stem extracts of Sorbonne; A-Ceb, aerial stem extracts of Ceb Dazzle; A-Lor, aerial stem extracts of L.formolongo.; L-Sor, leaf extracts of Sorbonne; L-Ceb, leaf extracts of Ceb Dazzle; L-Lor, leaf extracts of L.formolongo; CK1, GR24 as positive control; CK2, Distilled water as negative control; CK3, Methanol as negative control. The dotted black lines represent 20% and 40% position, respectively. Error bars represent the standard error of the mean. Different small letters above the error bars indicate significant differences at 0.05 (ANOVA and Duncan's multiple range test). Matching letters in the same color column indicates that there were no significant differences between treatments.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4643976&req=5

pone.0142811.g003: Induction of O. aegyptiaca Pers. germination by aqueous and methanol extracts of lily underground (A) and above-ground (B) organs at the leaf expansion stage.Two types of organs were tested, (A) underground organs and (B) above-ground organs. Two types of extracts were tested, (A1 and B1) aqueous and (A2 and B2) methanol. Four concentrations of extracts were assessed: undiluted and 10-fold, 100-fold and 1000-fold dilutions. Abbreviations: R-Sor, root extracts of Sorbonne; R-Ceb, root extracts of Ceb Dazzle; R-Lor, root extracts of L. formolongo; B-Sor, bulb extracts of Sorbonne; B-Ceb, bulb extracts of Ceb Dazzle; B-Lor, bulb extracts of L. formolongo; S-Sor, scale leaf extracts of Sorbonne; S-Ceb, scale leaf extracts of Ceb Dazzle; S-Lor, scale leaf extracts of L. formolongo; P-Sor, prop root extracts of Sorbonne; P-Ceb, prop root extracts of Ceb Dazzle; P-Lor, prop root extracts of L.formolongo; A-Sor, aerial stem extracts of Sorbonne; A-Ceb, aerial stem extracts of Ceb Dazzle; A-Lor, aerial stem extracts of L.formolongo.; L-Sor, leaf extracts of Sorbonne; L-Ceb, leaf extracts of Ceb Dazzle; L-Lor, leaf extracts of L.formolongo; CK1, GR24 as positive control; CK2, Distilled water as negative control; CK3, Methanol as negative control. The dotted black lines represent 20% and 40% position, respectively. Error bars represent the standard error of the mean. Different small letters above the error bars indicate significant differences at 0.05 (ANOVA and Duncan's multiple range test). Matching letters in the same color column indicates that there were no significant differences between treatments.
Mentions: The highest germination rates were induced by 10-fold dilution of aqueous and methanol extracts from underground organs (Fig 3). Maximum germination rates induced by 10-fold dilution of both aqueous and methanol extracts by organ were as follows: bulb > scale leaf > root in both Ceb Dazzle and L. formolongo. Differences between organs was significant (P<0.05) (Fig 4) (S2 Table). Ceb Dazzle (45.1%) and L. formolongo (60.1%) bulbs had relatively stable, strong allelopathic effects on O. aegyptiaca Pers. seeds, which were similar to effects in the sprouting stage (Figs 1 and 4). Although there were no significant differences between the underground organs of the Sorbonne cultivar, the highest germination rate was induced by scale leaf (50.1%), which was increased beyond the rate induced by bulbs in the leaf expansion stage (Fig 3A2).

Bottom Line: Specifically, Sorbonne and Ceb Dazzle behaved with similar allelopathy, and the bulb, scale leaf and aerial stem exhibited stronger allelopathic effects on O. aegyptiaca Pers. germination compared to other organs.Aqueous L. formolongo leaf extracts may contain more stable, effective stimulants given that they induced the highest germination rate at 76.7% even though the extracts were serially diluted.We speculate that these organs may be advantageous in further isolating and purifying economical active substances that can be substitutes for GR24.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.

ABSTRACT
As a devastating holoparasitic weed, Orobanche aegyptiaca Persoon. (Egyptian broomrape) causes serious damage to agricultural production and threatens economic development, which has raised widespread concern. The present study was conducted to determine whether lilies have the potential to be used as 'trap crops' for controlling O. aegyptiaca Persoon. In the experiments, the ability of three popular lily cultivars (Lilium Oriental hybrids 'Sorbonne', Lilium LA (Longiflorum hybrids x Asiatic hybrids) hybrids 'Ceb Dazzle', and Lilium Longiflorum hybrids (L. formosanum x L. longiflorum) 'L. formolongo') to induce O. aegyptiaca Persoon. seed germination was assessed. Parts of the three lily cultivars, including the rhizosphere soil and underground and above-ground organs, all induced "suicidal germination" of parasitic O. aegyptiaca Persoon. seed at four growth stages. Specifically, Sorbonne and Ceb Dazzle behaved with similar allelopathy, and the bulb, scale leaf and aerial stem exhibited stronger allelopathic effects on O. aegyptiaca Pers. germination compared to other organs. Aqueous L. formolongo leaf extracts may contain more stable, effective stimulants given that they induced the highest germination rate at 76.7% even though the extracts were serially diluted. We speculate that these organs may be advantageous in further isolating and purifying economical active substances that can be substitutes for GR24. These results indicate that lilies have the potential to be used as a trap crops or can be processed into green herbicide formulations that can be applied in agriculture production to rapidly deplete the seed bank of O. aegyptiaca Persoon. parasitic weeds in soil.

Show MeSH
Related in: MedlinePlus