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Evidence of Field-Evolved Resistance to Bifenthrin in Western Corn Rootworm (Diabrotica virgifera virgifera LeConte) Populations in Western Nebraska and Kansas.

Pereira AE, Wang H, Zukoff SN, Meinke LJ, French BW, Siegfried BD - PLoS ONE (2015)

Bottom Line: Concentration-response bioassays were performed to estimate the baseline susceptibility.This study provides evidence that resistance to bifenthrin is evolving in field populations that have been exposed for multiple years to pyrethroid insecticides.Implications to sustainable rootworm management are discussed.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America.

ABSTRACT
Pyrethroid insecticides have been used to control larvae or adults of the western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte, a key pest of field corn in the United States. In response to reports of reduced efficacy of pyrethroids in WCR management programs in southwestern areas of Nebraska and Kansas the present research was designed to establish a baseline of susceptibility to the pyrethroid insecticide, bifenthrin, using susceptible laboratory populations and to compare this baseline with susceptibility of field populations. Concentration-response bioassays were performed to estimate the baseline susceptibility. From the baseline data, a diagnostic concentration (LC99) was determined and used to test adults of both laboratory and field populations. Larval susceptibility was also tested using both laboratory and field populations. Significant differences were recorded in adult and larval susceptibility among WCR field and laboratory populations. The highest LC50 for WCR adults was observed in populations from Keith 2 and Chase Counties, NE, with LC50s of 2.2 and 1.38 μg/vial, respectively, and Finney County 1, KS, with 1.43 μg/vial, as compared to a laboratory non-diapause population (0.24 μg/vial). For larvae, significant differences between WCR field and laboratory populations were also recorded. Significant differences in mortalities at the diagnostic bifenthrin concentration (LC99) were observed among WCR adult populations with western Corn Belt populations exhibiting lower susceptibility to bifenthrin, especially in southwestern Nebraska and southwestern Kansas. This study provides evidence that resistance to bifenthrin is evolving in field populations that have been exposed for multiple years to pyrethroid insecticides. Implications to sustainable rootworm management are discussed.

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Related in: MedlinePlus

Probit mortality plots of bifenthrin determined by lethal concentration bioassays for adult WCR field populations from Keith and Perkins Counties, NE and a non-diapause laboratory colony (CCh) considered susceptible.Field collections and bioassays performed in 2014. Vertical dash-line represents the diagnostic concentration (LC99) of 0.77 μg/vial calculated from the 10 pooled lab colonies (Table 1).
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pone.0142299.g004: Probit mortality plots of bifenthrin determined by lethal concentration bioassays for adult WCR field populations from Keith and Perkins Counties, NE and a non-diapause laboratory colony (CCh) considered susceptible.Field collections and bioassays performed in 2014. Vertical dash-line represents the diagnostic concentration (LC99) of 0.77 μg/vial calculated from the 10 pooled lab colonies (Table 1).

Mentions: Complete dose-response bioassays for two field populations that exhibited reduced mortality at the diagnostic concentration (Keith 2 and Perkins 2) were conducted to estimate the LC50 as previously described and compared with a non-diapause strain (CCh) to determine a resistance ratio (Fig 4).


Evidence of Field-Evolved Resistance to Bifenthrin in Western Corn Rootworm (Diabrotica virgifera virgifera LeConte) Populations in Western Nebraska and Kansas.

Pereira AE, Wang H, Zukoff SN, Meinke LJ, French BW, Siegfried BD - PLoS ONE (2015)

Probit mortality plots of bifenthrin determined by lethal concentration bioassays for adult WCR field populations from Keith and Perkins Counties, NE and a non-diapause laboratory colony (CCh) considered susceptible.Field collections and bioassays performed in 2014. Vertical dash-line represents the diagnostic concentration (LC99) of 0.77 μg/vial calculated from the 10 pooled lab colonies (Table 1).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4643967&req=5

pone.0142299.g004: Probit mortality plots of bifenthrin determined by lethal concentration bioassays for adult WCR field populations from Keith and Perkins Counties, NE and a non-diapause laboratory colony (CCh) considered susceptible.Field collections and bioassays performed in 2014. Vertical dash-line represents the diagnostic concentration (LC99) of 0.77 μg/vial calculated from the 10 pooled lab colonies (Table 1).
Mentions: Complete dose-response bioassays for two field populations that exhibited reduced mortality at the diagnostic concentration (Keith 2 and Perkins 2) were conducted to estimate the LC50 as previously described and compared with a non-diapause strain (CCh) to determine a resistance ratio (Fig 4).

Bottom Line: Concentration-response bioassays were performed to estimate the baseline susceptibility.This study provides evidence that resistance to bifenthrin is evolving in field populations that have been exposed for multiple years to pyrethroid insecticides.Implications to sustainable rootworm management are discussed.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America.

ABSTRACT
Pyrethroid insecticides have been used to control larvae or adults of the western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte, a key pest of field corn in the United States. In response to reports of reduced efficacy of pyrethroids in WCR management programs in southwestern areas of Nebraska and Kansas the present research was designed to establish a baseline of susceptibility to the pyrethroid insecticide, bifenthrin, using susceptible laboratory populations and to compare this baseline with susceptibility of field populations. Concentration-response bioassays were performed to estimate the baseline susceptibility. From the baseline data, a diagnostic concentration (LC99) was determined and used to test adults of both laboratory and field populations. Larval susceptibility was also tested using both laboratory and field populations. Significant differences were recorded in adult and larval susceptibility among WCR field and laboratory populations. The highest LC50 for WCR adults was observed in populations from Keith 2 and Chase Counties, NE, with LC50s of 2.2 and 1.38 μg/vial, respectively, and Finney County 1, KS, with 1.43 μg/vial, as compared to a laboratory non-diapause population (0.24 μg/vial). For larvae, significant differences between WCR field and laboratory populations were also recorded. Significant differences in mortalities at the diagnostic bifenthrin concentration (LC99) were observed among WCR adult populations with western Corn Belt populations exhibiting lower susceptibility to bifenthrin, especially in southwestern Nebraska and southwestern Kansas. This study provides evidence that resistance to bifenthrin is evolving in field populations that have been exposed for multiple years to pyrethroid insecticides. Implications to sustainable rootworm management are discussed.

Show MeSH
Related in: MedlinePlus