Limits...
Detection and Characterization of Metastatic Cancer Cells in the Mesogastrium of Gastric Cancer Patients.

Xie D, Liu L, Osaiweran H, Yu C, Sheng F, Gao C, Hu J, Gong J - PLoS ONE (2015)

Bottom Line: Gastric cancer is the second leading cause of cancer death worldwide.Metastasis V was closely associated with tumor invasion depth, along with a number of positive lymph node metastasis.The prognosis of patients with Metastasis V was significantly (P<0.05) worse than those with tumor cell-free mesogastrium.

View Article: PubMed Central - PubMed

Affiliation: Tongji Cancer Research Institute, Tongji Hospital, Tongji Medical College in Huazhong University of Science and Technology, Wuhan, Hubei, China.

ABSTRACT
Gastric cancer is the second leading cause of cancer death worldwide. Here, we propose a novel type of tumor metastasis designated as Metastasis V in gastric cancer. Metastasis V is defined as the appearance of cancer cells in the mesogastrium with perigastric adipose tissue. To detect its incidence and characterize its clinic pathological features, large cross sectional tissue analysis of mesogastrium from 74 patients were used. Metastasis V was detected in 1 of 40 (2.5%) patients with early gastric cancer, 8 of 34 (24%) patients with advanced gastric cancer. The mean distance of Metastasis V from gastric wall was approximately 2.6 cm. Metastasis V was closely associated with tumor invasion depth, along with a number of positive lymph node metastasis. The prognosis of patients with Metastasis V was significantly (P<0.05) worse than those with tumor cell-free mesogastrium. These findings indicate that by using whole-sectional analysis, Metastasis V can be detected in the mesogastrium of gastric cancer patients, and also suggests that it may be a risk factor for patient survival after radical surgery.

Show MeSH

Related in: MedlinePlus

E-cadherin and DAB2IP expression in normal gastric mucosa, primary gastric tumors and Metastasis V within the mesogastrium.(A) Representative IHC staining for DAB2IP and E-cadherin from the same patient. (B) The relative quantitative analysis of E-cadherin and DAB2IP expression. One asterisk indicated statistical significance in normal mucosa vs. primary tumors (*, P < 0.01). Two asterisks indicated statistical significance in primary tumors vs. mesogastrium (**, P<0.01).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4643961&req=5

pone.0142970.g004: E-cadherin and DAB2IP expression in normal gastric mucosa, primary gastric tumors and Metastasis V within the mesogastrium.(A) Representative IHC staining for DAB2IP and E-cadherin from the same patient. (B) The relative quantitative analysis of E-cadherin and DAB2IP expression. One asterisk indicated statistical significance in normal mucosa vs. primary tumors (*, P < 0.01). Two asterisks indicated statistical significance in primary tumors vs. mesogastrium (**, P<0.01).

Mentions: In order to evaluate the clinical significance of the results and develop potential therapeutic target for Metastasis V, it was necessary to investigate the underlying molecular mechanisms. E-cadherin is regarded as a major marker for EMT (epithelial-to-mesenchymal transition), known as a critical process in the biology of cancer metastasis[14]. Our previous studies demonstrated that the loss of DAB2IP expression initiated EMT and promoted tumor invasion and metastasis[15]. In this study, the mesogastrium metastasis and its matching primary tumor and adjacent normal tissue were immunostained for DAB2IP and E-cadherin. Our results showed that the expression of both DAB2IP and E-cadherin decreased in the mesogastrium metastasis compared with that in the matched primary tumor and the adjacent normal tissue (Fig 4), which suggests that DAB2IP-regulated EMT may play a role in Metastasis V. Since the downstream of DAB2IP-mediated Wnt pathway is the activation of transcriptional factors such as ß-catenin and p65, we also detected the expression of ß-catenin and p65. Our results showed that the expression of both ß-catenin and p65 increased (as well as nuclear localization) in the mesogastrium metastasis compared with that in primary tumor (S1 Fig). The exact regulatory mechanisms, however, need to be further investigated.


Detection and Characterization of Metastatic Cancer Cells in the Mesogastrium of Gastric Cancer Patients.

Xie D, Liu L, Osaiweran H, Yu C, Sheng F, Gao C, Hu J, Gong J - PLoS ONE (2015)

E-cadherin and DAB2IP expression in normal gastric mucosa, primary gastric tumors and Metastasis V within the mesogastrium.(A) Representative IHC staining for DAB2IP and E-cadherin from the same patient. (B) The relative quantitative analysis of E-cadherin and DAB2IP expression. One asterisk indicated statistical significance in normal mucosa vs. primary tumors (*, P < 0.01). Two asterisks indicated statistical significance in primary tumors vs. mesogastrium (**, P<0.01).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4643961&req=5

pone.0142970.g004: E-cadherin and DAB2IP expression in normal gastric mucosa, primary gastric tumors and Metastasis V within the mesogastrium.(A) Representative IHC staining for DAB2IP and E-cadherin from the same patient. (B) The relative quantitative analysis of E-cadherin and DAB2IP expression. One asterisk indicated statistical significance in normal mucosa vs. primary tumors (*, P < 0.01). Two asterisks indicated statistical significance in primary tumors vs. mesogastrium (**, P<0.01).
Mentions: In order to evaluate the clinical significance of the results and develop potential therapeutic target for Metastasis V, it was necessary to investigate the underlying molecular mechanisms. E-cadherin is regarded as a major marker for EMT (epithelial-to-mesenchymal transition), known as a critical process in the biology of cancer metastasis[14]. Our previous studies demonstrated that the loss of DAB2IP expression initiated EMT and promoted tumor invasion and metastasis[15]. In this study, the mesogastrium metastasis and its matching primary tumor and adjacent normal tissue were immunostained for DAB2IP and E-cadherin. Our results showed that the expression of both DAB2IP and E-cadherin decreased in the mesogastrium metastasis compared with that in the matched primary tumor and the adjacent normal tissue (Fig 4), which suggests that DAB2IP-regulated EMT may play a role in Metastasis V. Since the downstream of DAB2IP-mediated Wnt pathway is the activation of transcriptional factors such as ß-catenin and p65, we also detected the expression of ß-catenin and p65. Our results showed that the expression of both ß-catenin and p65 increased (as well as nuclear localization) in the mesogastrium metastasis compared with that in primary tumor (S1 Fig). The exact regulatory mechanisms, however, need to be further investigated.

Bottom Line: Gastric cancer is the second leading cause of cancer death worldwide.Metastasis V was closely associated with tumor invasion depth, along with a number of positive lymph node metastasis.The prognosis of patients with Metastasis V was significantly (P<0.05) worse than those with tumor cell-free mesogastrium.

View Article: PubMed Central - PubMed

Affiliation: Tongji Cancer Research Institute, Tongji Hospital, Tongji Medical College in Huazhong University of Science and Technology, Wuhan, Hubei, China.

ABSTRACT
Gastric cancer is the second leading cause of cancer death worldwide. Here, we propose a novel type of tumor metastasis designated as Metastasis V in gastric cancer. Metastasis V is defined as the appearance of cancer cells in the mesogastrium with perigastric adipose tissue. To detect its incidence and characterize its clinic pathological features, large cross sectional tissue analysis of mesogastrium from 74 patients were used. Metastasis V was detected in 1 of 40 (2.5%) patients with early gastric cancer, 8 of 34 (24%) patients with advanced gastric cancer. The mean distance of Metastasis V from gastric wall was approximately 2.6 cm. Metastasis V was closely associated with tumor invasion depth, along with a number of positive lymph node metastasis. The prognosis of patients with Metastasis V was significantly (P<0.05) worse than those with tumor cell-free mesogastrium. These findings indicate that by using whole-sectional analysis, Metastasis V can be detected in the mesogastrium of gastric cancer patients, and also suggests that it may be a risk factor for patient survival after radical surgery.

Show MeSH
Related in: MedlinePlus