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A dietary phytochemical alters caste-associated gene expression in honey bees.

Mao W, Schuler MA, Berenbaum MR - Sci Adv (2015)

Bottom Line: RNA-Seq analysis demonstrated that p-coumaric acid, which is ubiquitous in honey and beebread, differentially regulates genes involved in caste determination.Rearing larvae in vitro on a royal jelly diet to which p-coumaric acid has been added produces adults with reduced ovary development.Thus, consuming royal jelly exclusively not only enriches the diet of queen-destined larvae but also may protect them from inhibitory effects of phytochemicals present in the honey and beebread fed to worker-destined larvae.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, University of Illinois at Urbana-Champaign, Urbana, IL 61801-3795, USA.

ABSTRACT
In the eusocial honey bee Apis mellifera, with reproductive queens and sterile workers, a female larva's developmental fate depends on its diet; nurse bees feed queen-destined larvae exclusively royal jelly, a glandular secretion, but worker-destined larvae receive royal jelly for 3 days and subsequently jelly to which honey and beebread are added. RNA-Seq analysis demonstrated that p-coumaric acid, which is ubiquitous in honey and beebread, differentially regulates genes involved in caste determination. Rearing larvae in vitro on a royal jelly diet to which p-coumaric acid has been added produces adults with reduced ovary development. Thus, consuming royal jelly exclusively not only enriches the diet of queen-destined larvae but also may protect them from inhibitory effects of phytochemicals present in the honey and beebread fed to worker-destined larvae.

No MeSH data available.


Related in: MedlinePlus

Hippo signaling pathway.Up-regulated genes appear in red, and down-regulated genes appear in green. For specific fold change and P values, see table S3.
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Figure 3: Hippo signaling pathway.Up-regulated genes appear in red, and down-regulated genes appear in green. For specific fold change and P values, see table S3.

Mentions: To characterize larval response profiles induced by p-coumaric acid at the level of a single pathway, we performed an RNA-Seq data pathway and gene-set analysis by using the two R packages GAGE and Pathview (21, 22). Among the two gene sets of signal transduction and metabolism extracted from the KEGG pathway database (www.genome.jpkeggpathway.html), only the Hippo signaling pathway, which contributes to regulating organ size in animals, was enriched (P < 0.1) (Fig. 3), suggesting that p-coumaric acid influences organ size in developing larvae. In this pathway, p-coumaric acid altered the expression of 26 genes among the 46 total genes (54.2%) (table S4). Among the five down-regulated genes, the only fold change less than −2.00 was that of Mats (−2.04). In contrast, 7 of the 21 up-regulated genes had fold changes greater than 2.00; these included Ds, Ft, Atrophin, Zyx, Ex, dJub, and Km.


A dietary phytochemical alters caste-associated gene expression in honey bees.

Mao W, Schuler MA, Berenbaum MR - Sci Adv (2015)

Hippo signaling pathway.Up-regulated genes appear in red, and down-regulated genes appear in green. For specific fold change and P values, see table S3.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4643792&req=5

Figure 3: Hippo signaling pathway.Up-regulated genes appear in red, and down-regulated genes appear in green. For specific fold change and P values, see table S3.
Mentions: To characterize larval response profiles induced by p-coumaric acid at the level of a single pathway, we performed an RNA-Seq data pathway and gene-set analysis by using the two R packages GAGE and Pathview (21, 22). Among the two gene sets of signal transduction and metabolism extracted from the KEGG pathway database (www.genome.jpkeggpathway.html), only the Hippo signaling pathway, which contributes to regulating organ size in animals, was enriched (P < 0.1) (Fig. 3), suggesting that p-coumaric acid influences organ size in developing larvae. In this pathway, p-coumaric acid altered the expression of 26 genes among the 46 total genes (54.2%) (table S4). Among the five down-regulated genes, the only fold change less than −2.00 was that of Mats (−2.04). In contrast, 7 of the 21 up-regulated genes had fold changes greater than 2.00; these included Ds, Ft, Atrophin, Zyx, Ex, dJub, and Km.

Bottom Line: RNA-Seq analysis demonstrated that p-coumaric acid, which is ubiquitous in honey and beebread, differentially regulates genes involved in caste determination.Rearing larvae in vitro on a royal jelly diet to which p-coumaric acid has been added produces adults with reduced ovary development.Thus, consuming royal jelly exclusively not only enriches the diet of queen-destined larvae but also may protect them from inhibitory effects of phytochemicals present in the honey and beebread fed to worker-destined larvae.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, University of Illinois at Urbana-Champaign, Urbana, IL 61801-3795, USA.

ABSTRACT
In the eusocial honey bee Apis mellifera, with reproductive queens and sterile workers, a female larva's developmental fate depends on its diet; nurse bees feed queen-destined larvae exclusively royal jelly, a glandular secretion, but worker-destined larvae receive royal jelly for 3 days and subsequently jelly to which honey and beebread are added. RNA-Seq analysis demonstrated that p-coumaric acid, which is ubiquitous in honey and beebread, differentially regulates genes involved in caste determination. Rearing larvae in vitro on a royal jelly diet to which p-coumaric acid has been added produces adults with reduced ovary development. Thus, consuming royal jelly exclusively not only enriches the diet of queen-destined larvae but also may protect them from inhibitory effects of phytochemicals present in the honey and beebread fed to worker-destined larvae.

No MeSH data available.


Related in: MedlinePlus