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Conjugative transfer of a derivative of the IncP-1α plasmid RP4 and establishment of transconjugants in the indigenous bacterial community of poplar plants.

Ulrich A, Becker R, Ulrich K, Ewald D - FEMS Microbiol. Lett. (2015)

Bottom Line: The increased colonization of the cuttings by indigenous bacteria at stem elongation seemed to strongly compete with the introduced strains.Otherwise, the phenological stage of the plants as well as the density of the indigenous recipients could serve as the driver for a more frequent conjugative plasmid transfer.A phylogenetic assignment of transconjugants indicated the transfer of RP4-Tn-luc into six genera of Proteobacteria, mainly Sphingomonas, Stenotrophomonas and Xanthomonas.

View Article: PubMed Central - PubMed

Affiliation: Leibniz Centre for Agricultural Landscape Research (ZALF), Institute for Landscape Biogeochemistry, D-15374 Müncheberg, Germany aulrich@zalf.de.

No MeSH data available.


Dendrogram showing the similarity between the transconjugants and related bacterial species. The phylogenetic tree was generated using the neighbor-joining algorithm. The 16S rRNA gene sequence of E. coli (J01695) was used as an outgroup. Type strains of the bacterial species were used for the comparison; the sequence accession numbers are provided in brackets. Numbers at nodes indicate levels of bootstrap support >75%. Strains used for inoculation are underlined. 16S rRNA genotypes Rhizobium 1 were represented by S169-III-8 and S169-34, Rhizobium 2 by P9-10, Sphingomonas 1 by P9-50, Sphingomonas 2 by S169-9, Stenotrophomonas 1 by S169-III-5 and Stenotrophomonas 2 by P9-23. The 16S rRNA gene sequences of the transconjugants written in bold letters were deposited in the EMBL database (HE652088–HE652100).
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fig1: Dendrogram showing the similarity between the transconjugants and related bacterial species. The phylogenetic tree was generated using the neighbor-joining algorithm. The 16S rRNA gene sequence of E. coli (J01695) was used as an outgroup. Type strains of the bacterial species were used for the comparison; the sequence accession numbers are provided in brackets. Numbers at nodes indicate levels of bootstrap support >75%. Strains used for inoculation are underlined. 16S rRNA genotypes Rhizobium 1 were represented by S169-III-8 and S169-34, Rhizobium 2 by P9-10, Sphingomonas 1 by P9-50, Sphingomonas 2 by S169-9, Stenotrophomonas 1 by S169-III-5 and Stenotrophomonas 2 by P9-23. The 16S rRNA gene sequences of the transconjugants written in bold letters were deposited in the EMBL database (HE652088–HE652100).

Mentions: As shown in Fig. 1, two of the Rhizobium genotypes were phylogenetically related to R. radiobacter (formerly Agrobacterium tumefaciens), whereas the third genotype showed a high similarity to R. soli. A phylogenetic differentiation was also found within the genus Sphingomonas. Most isolates of this genus showed homology to S. yanoikuyae, and two isolates were related to S. faenia. The genus Methylobacterium was represented by two isolates with a high similarity to M. mesophilicum. Betaproteobacteria represented by the genus Achromobacter were detected based on three isolates with nearest relationship to A. piechaudii. The remaining 16S rRNA genotypes were γ-Protebacteria of the genera Stenotrophomonas and Xanthomonas. Isolates identified as members of Xanthomonas and Stenotrophomonas 1 displayed identical RFLP patterns, but clearly differed in colony colour. Xanthomonas transconjugants detected after two different inoculations had identical 16S rRNA gene sequences and were most nearly related to X. campestris und X. populi. By contrast, transconjugants of the genus Stenotrophomonas represented two genotypes that were similar to S. rhizophilia or S. chelatiphaga, and each was distinguishable from the donor stain Stenotrophomonas 169-1 RP4-4-Tn-luc.


Conjugative transfer of a derivative of the IncP-1α plasmid RP4 and establishment of transconjugants in the indigenous bacterial community of poplar plants.

Ulrich A, Becker R, Ulrich K, Ewald D - FEMS Microbiol. Lett. (2015)

Dendrogram showing the similarity between the transconjugants and related bacterial species. The phylogenetic tree was generated using the neighbor-joining algorithm. The 16S rRNA gene sequence of E. coli (J01695) was used as an outgroup. Type strains of the bacterial species were used for the comparison; the sequence accession numbers are provided in brackets. Numbers at nodes indicate levels of bootstrap support >75%. Strains used for inoculation are underlined. 16S rRNA genotypes Rhizobium 1 were represented by S169-III-8 and S169-34, Rhizobium 2 by P9-10, Sphingomonas 1 by P9-50, Sphingomonas 2 by S169-9, Stenotrophomonas 1 by S169-III-5 and Stenotrophomonas 2 by P9-23. The 16S rRNA gene sequences of the transconjugants written in bold letters were deposited in the EMBL database (HE652088–HE652100).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4643746&req=5

fig1: Dendrogram showing the similarity between the transconjugants and related bacterial species. The phylogenetic tree was generated using the neighbor-joining algorithm. The 16S rRNA gene sequence of E. coli (J01695) was used as an outgroup. Type strains of the bacterial species were used for the comparison; the sequence accession numbers are provided in brackets. Numbers at nodes indicate levels of bootstrap support >75%. Strains used for inoculation are underlined. 16S rRNA genotypes Rhizobium 1 were represented by S169-III-8 and S169-34, Rhizobium 2 by P9-10, Sphingomonas 1 by P9-50, Sphingomonas 2 by S169-9, Stenotrophomonas 1 by S169-III-5 and Stenotrophomonas 2 by P9-23. The 16S rRNA gene sequences of the transconjugants written in bold letters were deposited in the EMBL database (HE652088–HE652100).
Mentions: As shown in Fig. 1, two of the Rhizobium genotypes were phylogenetically related to R. radiobacter (formerly Agrobacterium tumefaciens), whereas the third genotype showed a high similarity to R. soli. A phylogenetic differentiation was also found within the genus Sphingomonas. Most isolates of this genus showed homology to S. yanoikuyae, and two isolates were related to S. faenia. The genus Methylobacterium was represented by two isolates with a high similarity to M. mesophilicum. Betaproteobacteria represented by the genus Achromobacter were detected based on three isolates with nearest relationship to A. piechaudii. The remaining 16S rRNA genotypes were γ-Protebacteria of the genera Stenotrophomonas and Xanthomonas. Isolates identified as members of Xanthomonas and Stenotrophomonas 1 displayed identical RFLP patterns, but clearly differed in colony colour. Xanthomonas transconjugants detected after two different inoculations had identical 16S rRNA gene sequences and were most nearly related to X. campestris und X. populi. By contrast, transconjugants of the genus Stenotrophomonas represented two genotypes that were similar to S. rhizophilia or S. chelatiphaga, and each was distinguishable from the donor stain Stenotrophomonas 169-1 RP4-4-Tn-luc.

Bottom Line: The increased colonization of the cuttings by indigenous bacteria at stem elongation seemed to strongly compete with the introduced strains.Otherwise, the phenological stage of the plants as well as the density of the indigenous recipients could serve as the driver for a more frequent conjugative plasmid transfer.A phylogenetic assignment of transconjugants indicated the transfer of RP4-Tn-luc into six genera of Proteobacteria, mainly Sphingomonas, Stenotrophomonas and Xanthomonas.

View Article: PubMed Central - PubMed

Affiliation: Leibniz Centre for Agricultural Landscape Research (ZALF), Institute for Landscape Biogeochemistry, D-15374 Müncheberg, Germany aulrich@zalf.de.

No MeSH data available.