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Lung ultrasound is a reliable method for evaluating extravascular lung water volume in rodents.

Ma H, Huang D, Zhang M, Huang X, Ma S, Mao S, Li W, Chen Y, Guo L - BMC Anesthesiol (2015)

Bottom Line: Repeatability studies of the LUS technique (Bland-Altman plots) showed good intra-observer and inter-observer reproducibility.The data suggest that, in an experimental rat model of ALI, B lines score as assessed by LUS can provide an easy, semi-quantitative, noninvasive.Real-time index of EVLW which is strongly correlated to experimental gravimetric assessments.

View Article: PubMed Central - PubMed

Affiliation: The Department of Critical Care Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, Guangdong, People's Republic of China.

ABSTRACT

Backgroud: Lung ultrasound (LUS) can diagnose extravacular lung water (EVLW) through the visualization of B lines in both humans and large animals. However, there are no published data on the use of ultrasound to detect EVLW in rats, the gold standard to evaluate of EVLW in rats is post-mortem gravimetric analysis. The present study was designed to determine the similarity between lung sonography and gravimetric measurements of EVLW in rats in an acute lung injury (ALI) model.

Methods: Thirty male Sprague-Dawley rats were randomized into control and experimental groups. The B lines were measured byLUS at baseline. ALI was induced by the intravenous administration of oleic acid (OA) at a dose of 9 ul/100 mg, and controls were injected the same amount of isotonic saline. After 1 h, B-lines were measured by LUS in each rat following the induction of ALI. At the end of each experiment, both lungs were dissected, weighed and dried to determine wet/dry weight ratio according to the standard gravimetric methodology. Lung samples from three rats in each group were examined histologically.

Results: B-lines were present in all rats from experimental group at 1 h point after OA injection. The statistical correlation between the two methods of assessing EVLW provided an r = 0.834 (p < 0.001). Repeatability studies of the LUS technique (Bland-Altman plots) showed good intra-observer and inter-observer reproducibility.

Conclusion: The data suggest that, in an experimental rat model of ALI, B lines score as assessed by LUS can provide an easy, semi-quantitative, noninvasive. Real-time index of EVLW which is strongly correlated to experimental gravimetric assessments.

No MeSH data available.


Related in: MedlinePlus

How to calculate the B line score a 0 B line (Score = 0). b 1 B line (Score = 1). c 3 B lines (Score = 3). d Confluent B lines assesse the percentage of the rib space and divided it by ten. Score = 045 cm/0.9 cm × 10 = 5. e Confluent B lines present in more than one rib spaces: Add each score together to get the whole score in one scan. Score = 0.7 cm/2.8 cm × 10 + 0.7 cm/2.8 cm × 10 + 0.6 cm/2.8 cm × 10 = 10.15 ≈ 10. The whole score of the rat lung semiquantified by LUS is the sum of four scores in each scan
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Fig2: How to calculate the B line score a 0 B line (Score = 0). b 1 B line (Score = 1). c 3 B lines (Score = 3). d Confluent B lines assesse the percentage of the rib space and divided it by ten. Score = 045 cm/0.9 cm × 10 = 5. e Confluent B lines present in more than one rib spaces: Add each score together to get the whole score in one scan. Score = 0.7 cm/2.8 cm × 10 + 0.7 cm/2.8 cm × 10 + 0.6 cm/2.8 cm × 10 = 10.15 ≈ 10. The whole score of the rat lung semiquantified by LUS is the sum of four scores in each scan

Mentions: For each scan, the A lines or B lines was recorded at baseline [11], and 1 h after the injection of OA or NS. The A lines were present as repetitive horizontal artifacts parallel to the pleural line and arising from it, caused by the preponderance of air over liquid in the lung parenchyma. The presence of A lines was given a score of zero, indicating a normal LUS pattern. The B lines defined as discrete laser-like vertical hyperechoic reverberation artifacts that arise from the pleural line (previously described as “comet tails”), extending to the bottom of the screen without fading, and move synchronously with lung sliding [10]. The B lines score was evaluated as follows: at each intercostal space, the number of B lines was counted (ranging from zero to ten, corresponding to a confluency of signal) and then the percentage of the rib space occupied by B-lines was determined and divided by ten (see Fig. 2). The B lines scores was evaluated by two physicians trained in the evaluation of chest ultrasound, and a consensus was reached when there was disagreement.Fig. 2


Lung ultrasound is a reliable method for evaluating extravascular lung water volume in rodents.

Ma H, Huang D, Zhang M, Huang X, Ma S, Mao S, Li W, Chen Y, Guo L - BMC Anesthesiol (2015)

How to calculate the B line score a 0 B line (Score = 0). b 1 B line (Score = 1). c 3 B lines (Score = 3). d Confluent B lines assesse the percentage of the rib space and divided it by ten. Score = 045 cm/0.9 cm × 10 = 5. e Confluent B lines present in more than one rib spaces: Add each score together to get the whole score in one scan. Score = 0.7 cm/2.8 cm × 10 + 0.7 cm/2.8 cm × 10 + 0.6 cm/2.8 cm × 10 = 10.15 ≈ 10. The whole score of the rat lung semiquantified by LUS is the sum of four scores in each scan
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4643534&req=5

Fig2: How to calculate the B line score a 0 B line (Score = 0). b 1 B line (Score = 1). c 3 B lines (Score = 3). d Confluent B lines assesse the percentage of the rib space and divided it by ten. Score = 045 cm/0.9 cm × 10 = 5. e Confluent B lines present in more than one rib spaces: Add each score together to get the whole score in one scan. Score = 0.7 cm/2.8 cm × 10 + 0.7 cm/2.8 cm × 10 + 0.6 cm/2.8 cm × 10 = 10.15 ≈ 10. The whole score of the rat lung semiquantified by LUS is the sum of four scores in each scan
Mentions: For each scan, the A lines or B lines was recorded at baseline [11], and 1 h after the injection of OA or NS. The A lines were present as repetitive horizontal artifacts parallel to the pleural line and arising from it, caused by the preponderance of air over liquid in the lung parenchyma. The presence of A lines was given a score of zero, indicating a normal LUS pattern. The B lines defined as discrete laser-like vertical hyperechoic reverberation artifacts that arise from the pleural line (previously described as “comet tails”), extending to the bottom of the screen without fading, and move synchronously with lung sliding [10]. The B lines score was evaluated as follows: at each intercostal space, the number of B lines was counted (ranging from zero to ten, corresponding to a confluency of signal) and then the percentage of the rib space occupied by B-lines was determined and divided by ten (see Fig. 2). The B lines scores was evaluated by two physicians trained in the evaluation of chest ultrasound, and a consensus was reached when there was disagreement.Fig. 2

Bottom Line: Repeatability studies of the LUS technique (Bland-Altman plots) showed good intra-observer and inter-observer reproducibility.The data suggest that, in an experimental rat model of ALI, B lines score as assessed by LUS can provide an easy, semi-quantitative, noninvasive.Real-time index of EVLW which is strongly correlated to experimental gravimetric assessments.

View Article: PubMed Central - PubMed

Affiliation: The Department of Critical Care Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, Guangdong, People's Republic of China.

ABSTRACT

Backgroud: Lung ultrasound (LUS) can diagnose extravacular lung water (EVLW) through the visualization of B lines in both humans and large animals. However, there are no published data on the use of ultrasound to detect EVLW in rats, the gold standard to evaluate of EVLW in rats is post-mortem gravimetric analysis. The present study was designed to determine the similarity between lung sonography and gravimetric measurements of EVLW in rats in an acute lung injury (ALI) model.

Methods: Thirty male Sprague-Dawley rats were randomized into control and experimental groups. The B lines were measured byLUS at baseline. ALI was induced by the intravenous administration of oleic acid (OA) at a dose of 9 ul/100 mg, and controls were injected the same amount of isotonic saline. After 1 h, B-lines were measured by LUS in each rat following the induction of ALI. At the end of each experiment, both lungs were dissected, weighed and dried to determine wet/dry weight ratio according to the standard gravimetric methodology. Lung samples from three rats in each group were examined histologically.

Results: B-lines were present in all rats from experimental group at 1 h point after OA injection. The statistical correlation between the two methods of assessing EVLW provided an r = 0.834 (p < 0.001). Repeatability studies of the LUS technique (Bland-Altman plots) showed good intra-observer and inter-observer reproducibility.

Conclusion: The data suggest that, in an experimental rat model of ALI, B lines score as assessed by LUS can provide an easy, semi-quantitative, noninvasive. Real-time index of EVLW which is strongly correlated to experimental gravimetric assessments.

No MeSH data available.


Related in: MedlinePlus