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Specific loss of CatSper function is sufficient to compromise fertilizing capacity of human spermatozoa.

Williams HL, Mansell S, Alasmari W, Brown SG, Wilson SM, Sutton KA, Miller MR, Lishko PV, Barratt CL, Publicover SJ, Martins da Silva S - Hum. Reprod. (2015)

Bottom Line: A suboptimal Ca(2+) influx is significantly associated with, and more prevalent in, men with abnormal semen parameters, and is associated with reduced fertilizing capacity.As such it cannot be concluded that impaired CatSper function alone causes infertility or that CatSper blockade is a potential safe target for contraception.Not applicable.

View Article: PubMed Central - PubMed

Affiliation: Reproductive and Developmental Biology, Medical School, Ninewells Hospital, University of Dundee, Dundee DD1 9SY, UK Assisted Conception Unit, NHS Tayside, Ninewells Hospital, Dundee DD1 9SY, UK.

No MeSH data available.


Related in: MedlinePlus

Calcium response to progesterone, hyperactivation in response to 4-AP and penetration into viscous media for spermatozoa from donors and IVF patients. (A) Peak calcium response to progesterone (progesterone addition indicated by the arrow) was not significantly different between donors and IVF patients (donor n = 34 ejaculates from 23 different donors, IVF n = 102), however, the plateau phase response (mean of the last 12 s of recording) was (donor versus IVF peak calcium response to progesterone, P = 0.003, Student's unpaired t-test). (B and C) 4-aminopyridine (4-AP) significantly increased hyperactivation (HA) in spermatozoa from both donors and IVF patients (P < 0.001 versus control, Student's paired t-test). HA assay donor n = 35, IVF n = 66. (D and E) Penetration into viscous media was higher after stimulation with progesterone for both donors and IVF patients (progesterone versus control, donor n = 18, P < 0.05; IVF n = 16, P < 0.05, one-way analysis of variance). Asterisk denotes statistical significance at the P < 0.05 level.
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DEV243F1: Calcium response to progesterone, hyperactivation in response to 4-AP and penetration into viscous media for spermatozoa from donors and IVF patients. (A) Peak calcium response to progesterone (progesterone addition indicated by the arrow) was not significantly different between donors and IVF patients (donor n = 34 ejaculates from 23 different donors, IVF n = 102), however, the plateau phase response (mean of the last 12 s of recording) was (donor versus IVF peak calcium response to progesterone, P = 0.003, Student's unpaired t-test). (B and C) 4-aminopyridine (4-AP) significantly increased hyperactivation (HA) in spermatozoa from both donors and IVF patients (P < 0.001 versus control, Student's paired t-test). HA assay donor n = 35, IVF n = 66. (D and E) Penetration into viscous media was higher after stimulation with progesterone for both donors and IVF patients (progesterone versus control, donor n = 18, P < 0.05; IVF n = 16, P < 0.05, one-way analysis of variance). Asterisk denotes statistical significance at the P < 0.05 level.

Mentions: The majority of IVF patients (101/102; 99%) and donors (22/23; 95.7%) exhibited a biphasic [Ca2+]i response as previously described (Alasmari et al, 2013a). The [Ca2+]i transient amplitude was similar in cells from donors and patients attending for IVF treatment (Fig. 1A; donors = 2.57 ± 0.68 [n = 34 ejaculates from 23 different donors], IVF patients = 2.66 ± 0.68 [n = 102 patients], P = 0.63) but the plateau phase of the [Ca2+]i response was greater in IVF patients (P = 0.003). In total, 9/10 patients with previous low/failed fertilization at IVF showed a normal Ca2+ response.Figure 1


Specific loss of CatSper function is sufficient to compromise fertilizing capacity of human spermatozoa.

Williams HL, Mansell S, Alasmari W, Brown SG, Wilson SM, Sutton KA, Miller MR, Lishko PV, Barratt CL, Publicover SJ, Martins da Silva S - Hum. Reprod. (2015)

Calcium response to progesterone, hyperactivation in response to 4-AP and penetration into viscous media for spermatozoa from donors and IVF patients. (A) Peak calcium response to progesterone (progesterone addition indicated by the arrow) was not significantly different between donors and IVF patients (donor n = 34 ejaculates from 23 different donors, IVF n = 102), however, the plateau phase response (mean of the last 12 s of recording) was (donor versus IVF peak calcium response to progesterone, P = 0.003, Student's unpaired t-test). (B and C) 4-aminopyridine (4-AP) significantly increased hyperactivation (HA) in spermatozoa from both donors and IVF patients (P < 0.001 versus control, Student's paired t-test). HA assay donor n = 35, IVF n = 66. (D and E) Penetration into viscous media was higher after stimulation with progesterone for both donors and IVF patients (progesterone versus control, donor n = 18, P < 0.05; IVF n = 16, P < 0.05, one-way analysis of variance). Asterisk denotes statistical significance at the P < 0.05 level.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4643530&req=5

DEV243F1: Calcium response to progesterone, hyperactivation in response to 4-AP and penetration into viscous media for spermatozoa from donors and IVF patients. (A) Peak calcium response to progesterone (progesterone addition indicated by the arrow) was not significantly different between donors and IVF patients (donor n = 34 ejaculates from 23 different donors, IVF n = 102), however, the plateau phase response (mean of the last 12 s of recording) was (donor versus IVF peak calcium response to progesterone, P = 0.003, Student's unpaired t-test). (B and C) 4-aminopyridine (4-AP) significantly increased hyperactivation (HA) in spermatozoa from both donors and IVF patients (P < 0.001 versus control, Student's paired t-test). HA assay donor n = 35, IVF n = 66. (D and E) Penetration into viscous media was higher after stimulation with progesterone for both donors and IVF patients (progesterone versus control, donor n = 18, P < 0.05; IVF n = 16, P < 0.05, one-way analysis of variance). Asterisk denotes statistical significance at the P < 0.05 level.
Mentions: The majority of IVF patients (101/102; 99%) and donors (22/23; 95.7%) exhibited a biphasic [Ca2+]i response as previously described (Alasmari et al, 2013a). The [Ca2+]i transient amplitude was similar in cells from donors and patients attending for IVF treatment (Fig. 1A; donors = 2.57 ± 0.68 [n = 34 ejaculates from 23 different donors], IVF patients = 2.66 ± 0.68 [n = 102 patients], P = 0.63) but the plateau phase of the [Ca2+]i response was greater in IVF patients (P = 0.003). In total, 9/10 patients with previous low/failed fertilization at IVF showed a normal Ca2+ response.Figure 1

Bottom Line: A suboptimal Ca(2+) influx is significantly associated with, and more prevalent in, men with abnormal semen parameters, and is associated with reduced fertilizing capacity.As such it cannot be concluded that impaired CatSper function alone causes infertility or that CatSper blockade is a potential safe target for contraception.Not applicable.

View Article: PubMed Central - PubMed

Affiliation: Reproductive and Developmental Biology, Medical School, Ninewells Hospital, University of Dundee, Dundee DD1 9SY, UK Assisted Conception Unit, NHS Tayside, Ninewells Hospital, Dundee DD1 9SY, UK.

No MeSH data available.


Related in: MedlinePlus