Limits...
Six- and seven-dimensional experiments by combination of sparse random sampling and projection spectroscopy dedicated for backbone resonance assignment of intrinsically disordered proteins.

Żerko S, Koźmiński W - J. Biomol. NMR (2015)

Bottom Line: The resulted data sets could be processed as five-dimensional data using existing software.The novel experiments were successfully tested using 1 mM sample of α-synuclein on 600 and 800 MHz NMR spectrometers equipped with standard room temperature probes.The experiments allowed backbone assignment from a 1-day acquisition.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, Żwirki i Wigury 101, 02089, Warsaw, Poland.

ABSTRACT
Two novel six- and seven-dimensional NMR experiments are proposed. The new experiments employ non-uniform sampling that enables achieving high resolution in four indirectly detected dimensions and synchronous sampling in the additional dimensions using projection spectroscopy principle. The resulted data sets could be processed as five-dimensional data using existing software. The experiments facilitate resonance assignment of intrinsically disordered proteins. The novel experiments were successfully tested using 1 mM sample of α-synuclein on 600 and 800 MHz NMR spectrometers equipped with standard room temperature probes. The experiments allowed backbone assignment from a 1-day acquisition.

No MeSH data available.


Transfer efficiencies for auto-correlation and sequential peaks as a function of ΔN–CA delay length
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4642589&req=5

Fig2: Transfer efficiencies for auto-correlation and sequential peaks as a function of ΔN–CA delay length

Mentions: Assuming , values of 11, 7 and 35 Hz (Sattler et al. 1999), respectively, the optimal choice of ΔN–CA delay length for both high intensity of sequential signal (Ni−1(ω1), COi−2(ω2), COi−1(ω3), Ni(ω4), HiN(ω5)) and the suppression of auto-correlation signal (Ni(ω1), COi−1(ω2), COi−1(ω3), Ni(ω4), HiN(ω5)) can be obtained with the value of 54 ms (see Fig. 2), similar approach was also presented by Nováček et al. 2011. In the present work the auto-correlation peaks suppressed version was used.Fig. 2


Six- and seven-dimensional experiments by combination of sparse random sampling and projection spectroscopy dedicated for backbone resonance assignment of intrinsically disordered proteins.

Żerko S, Koźmiński W - J. Biomol. NMR (2015)

Transfer efficiencies for auto-correlation and sequential peaks as a function of ΔN–CA delay length
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4642589&req=5

Fig2: Transfer efficiencies for auto-correlation and sequential peaks as a function of ΔN–CA delay length
Mentions: Assuming , values of 11, 7 and 35 Hz (Sattler et al. 1999), respectively, the optimal choice of ΔN–CA delay length for both high intensity of sequential signal (Ni−1(ω1), COi−2(ω2), COi−1(ω3), Ni(ω4), HiN(ω5)) and the suppression of auto-correlation signal (Ni(ω1), COi−1(ω2), COi−1(ω3), Ni(ω4), HiN(ω5)) can be obtained with the value of 54 ms (see Fig. 2), similar approach was also presented by Nováček et al. 2011. In the present work the auto-correlation peaks suppressed version was used.Fig. 2

Bottom Line: The resulted data sets could be processed as five-dimensional data using existing software.The novel experiments were successfully tested using 1 mM sample of α-synuclein on 600 and 800 MHz NMR spectrometers equipped with standard room temperature probes.The experiments allowed backbone assignment from a 1-day acquisition.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, Żwirki i Wigury 101, 02089, Warsaw, Poland.

ABSTRACT
Two novel six- and seven-dimensional NMR experiments are proposed. The new experiments employ non-uniform sampling that enables achieving high resolution in four indirectly detected dimensions and synchronous sampling in the additional dimensions using projection spectroscopy principle. The resulted data sets could be processed as five-dimensional data using existing software. The experiments facilitate resonance assignment of intrinsically disordered proteins. The novel experiments were successfully tested using 1 mM sample of α-synuclein on 600 and 800 MHz NMR spectrometers equipped with standard room temperature probes. The experiments allowed backbone assignment from a 1-day acquisition.

No MeSH data available.