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Drosophila domino Exhibits Genetic Interactions with a Wide Spectrum of Chromatin Protein-Encoding Loci.

Ellis K, Friedman C, Yedvobnick B - PLoS ONE (2015)

Bottom Line: Here, the potential relationship of Domino protein to other chromatin-associated proteins has been investigated through a genetic interaction analysis.In several cases the genetic interactions are associated with changes in the level of cell death measured across the dorsal-ventral margin of the wing imaginal disc.These results highlight the broad realms of action of many chromatin proteins and suggest significant overlap with Domino function in fundamental cell processes, including cell proliferation, cell death and cell signaling.

View Article: PubMed Central - PubMed

Affiliation: Biology Department, Emory University, Atlanta, Georgia, United States of America.

ABSTRACT
The Drosophila domino gene encodes protein of the SWI2/SNF2 family that has widespread roles in transcription, replication, recombination and DNA repair. Here, the potential relationship of Domino protein to other chromatin-associated proteins has been investigated through a genetic interaction analysis. We scored for genetic modification of a domino wing margin phenotype through coexpression of RNAi directed against a set of previously characterized and more newly characterized chromatin-encoding loci. A set of other SWI2/SNF2 loci were also assayed for interaction with domino. Our results show that the majority of tested loci exhibit synergistic enhancement or suppression of the domino wing phenotype. Therefore, depression in domino function sensitizes the wing margin to alterations in the activity of numerous chromatin components. In several cases the genetic interactions are associated with changes in the level of cell death measured across the dorsal-ventral margin of the wing imaginal disc. These results highlight the broad realms of action of many chromatin proteins and suggest significant overlap with Domino function in fundamental cell processes, including cell proliferation, cell death and cell signaling.

No MeSH data available.


Related in: MedlinePlus

Genetic interactions of 12 SWI2/SNF2 protein encoding loci with domino.Wing mounts were prepared from the following Bloomington (BL) strains after crosses to C96-domR. Wings shown in panels C-N derived from crosses to TRiP strains. A. Wild Type wing BL3605 w1118, B. Control wing C96-domR/w1118, C. BL32845 ISWI, D. BL34665 CHD1, E. BL33708 INO80, F. BL32894 XNP, G. BL34520 BRM, H. BL32895 HEL89B, I. BL33709 MARCAL1, J. BL33891 ETL1, K.BL33419 Mi-2, L. BL34908 KISMET, M. BL33420 CHD3, N. BL33707 OKR.
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pone.0142635.g002: Genetic interactions of 12 SWI2/SNF2 protein encoding loci with domino.Wing mounts were prepared from the following Bloomington (BL) strains after crosses to C96-domR. Wings shown in panels C-N derived from crosses to TRiP strains. A. Wild Type wing BL3605 w1118, B. Control wing C96-domR/w1118, C. BL32845 ISWI, D. BL34665 CHD1, E. BL33708 INO80, F. BL32894 XNP, G. BL34520 BRM, H. BL32895 HEL89B, I. BL33709 MARCAL1, J. BL33891 ETL1, K.BL33419 Mi-2, L. BL34908 KISMET, M. BL33420 CHD3, N. BL33707 OKR.

Mentions: *Only 3 flies eclosed from the Mi-2 x C96-domR crosses and 5 wings could be scored, all of which were scalloped severely (Fig 2). The Mi-2 x C96-Gal4 control crosses produced 45 offspring, where 12/90 wings contained minor nicks, and 100% were curled.


Drosophila domino Exhibits Genetic Interactions with a Wide Spectrum of Chromatin Protein-Encoding Loci.

Ellis K, Friedman C, Yedvobnick B - PLoS ONE (2015)

Genetic interactions of 12 SWI2/SNF2 protein encoding loci with domino.Wing mounts were prepared from the following Bloomington (BL) strains after crosses to C96-domR. Wings shown in panels C-N derived from crosses to TRiP strains. A. Wild Type wing BL3605 w1118, B. Control wing C96-domR/w1118, C. BL32845 ISWI, D. BL34665 CHD1, E. BL33708 INO80, F. BL32894 XNP, G. BL34520 BRM, H. BL32895 HEL89B, I. BL33709 MARCAL1, J. BL33891 ETL1, K.BL33419 Mi-2, L. BL34908 KISMET, M. BL33420 CHD3, N. BL33707 OKR.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4640824&req=5

pone.0142635.g002: Genetic interactions of 12 SWI2/SNF2 protein encoding loci with domino.Wing mounts were prepared from the following Bloomington (BL) strains after crosses to C96-domR. Wings shown in panels C-N derived from crosses to TRiP strains. A. Wild Type wing BL3605 w1118, B. Control wing C96-domR/w1118, C. BL32845 ISWI, D. BL34665 CHD1, E. BL33708 INO80, F. BL32894 XNP, G. BL34520 BRM, H. BL32895 HEL89B, I. BL33709 MARCAL1, J. BL33891 ETL1, K.BL33419 Mi-2, L. BL34908 KISMET, M. BL33420 CHD3, N. BL33707 OKR.
Mentions: *Only 3 flies eclosed from the Mi-2 x C96-domR crosses and 5 wings could be scored, all of which were scalloped severely (Fig 2). The Mi-2 x C96-Gal4 control crosses produced 45 offspring, where 12/90 wings contained minor nicks, and 100% were curled.

Bottom Line: Here, the potential relationship of Domino protein to other chromatin-associated proteins has been investigated through a genetic interaction analysis.In several cases the genetic interactions are associated with changes in the level of cell death measured across the dorsal-ventral margin of the wing imaginal disc.These results highlight the broad realms of action of many chromatin proteins and suggest significant overlap with Domino function in fundamental cell processes, including cell proliferation, cell death and cell signaling.

View Article: PubMed Central - PubMed

Affiliation: Biology Department, Emory University, Atlanta, Georgia, United States of America.

ABSTRACT
The Drosophila domino gene encodes protein of the SWI2/SNF2 family that has widespread roles in transcription, replication, recombination and DNA repair. Here, the potential relationship of Domino protein to other chromatin-associated proteins has been investigated through a genetic interaction analysis. We scored for genetic modification of a domino wing margin phenotype through coexpression of RNAi directed against a set of previously characterized and more newly characterized chromatin-encoding loci. A set of other SWI2/SNF2 loci were also assayed for interaction with domino. Our results show that the majority of tested loci exhibit synergistic enhancement or suppression of the domino wing phenotype. Therefore, depression in domino function sensitizes the wing margin to alterations in the activity of numerous chromatin components. In several cases the genetic interactions are associated with changes in the level of cell death measured across the dorsal-ventral margin of the wing imaginal disc. These results highlight the broad realms of action of many chromatin proteins and suggest significant overlap with Domino function in fundamental cell processes, including cell proliferation, cell death and cell signaling.

No MeSH data available.


Related in: MedlinePlus