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Extraction and Identification of the Pigment in the Adductor Muscle Scar of Pacific Oyster Crassostrea gigas.

Hao S, Hou X, Wei L, Li J, Li Z, Wang X - PLoS ONE (2015)

Bottom Line: The maximum absorption of the pigment in the UV absorption spectrum within the range of 190-500 nm was observed between 210-220 nm.The UV absorbance decreased with increasing wavelength which was consistent with the UV spectral absorption characteristics of melanin.In addition, Fourier transform infrared spectroscopy scanning revealed characteristic absorption peaks that emerged near 3440 cm-1 and 1630 cm-1, which was consistent with infrared scanning features of eumelanin (a type of melanin).

View Article: PubMed Central - PubMed

Affiliation: School of Agriculture, Ludong University, Yantai, Shandong, China.

ABSTRACT
In this study, UV (ultraviolet) and IR (infrared radiation) spectral analysis were integrated to identify the pigment in the adductor muscle scar of the Pacific oyster Crassostrea gigas. The pigment was extracted from the adductor muscle scars of cleaned oyster shells that were pulverized, hydrolyzed in hot hydrochloric acid, purified with diethyl ether, and dissolved in 0.01 mL/L NaOH. The maximum absorption of the pigment in the UV absorption spectrum within the range of 190-500 nm was observed between 210-220 nm. The UV absorbance decreased with increasing wavelength which was consistent with the UV spectral absorption characteristics of melanin. In addition, Fourier transform infrared spectroscopy scanning revealed characteristic absorption peaks that emerged near 3440 cm-1 and 1630 cm-1, which was consistent with infrared scanning features of eumelanin (a type of melanin). This study has demonstrated for the first time that the pigment in the adductor muscle scar of the Pacific oyster is melanin, hinting that the adductor muscle could be another organ pigmenting the mollusc shell with melanin other than mantle.

No MeSH data available.


Related in: MedlinePlus

IR scanning spectra of the pigment extracted from the adductor muscle scar of Pacific oyster.‘T’ denotes the transmittance, ‘Wav’ the wavenumber.
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pone.0142439.g004: IR scanning spectra of the pigment extracted from the adductor muscle scar of Pacific oyster.‘T’ denotes the transmittance, ‘Wav’ the wavenumber.

Mentions: The IR spectrum of the pigment extracted from the adductor muscle scar of C. gigas is shown in Fig 4. The characteristic absorption peaks of the pigment were mainly distributed in the following three groups: 3500~3300 cm-1, 1620~1600 cm-1, and 1150~1000 cm-1.


Extraction and Identification of the Pigment in the Adductor Muscle Scar of Pacific Oyster Crassostrea gigas.

Hao S, Hou X, Wei L, Li J, Li Z, Wang X - PLoS ONE (2015)

IR scanning spectra of the pigment extracted from the adductor muscle scar of Pacific oyster.‘T’ denotes the transmittance, ‘Wav’ the wavenumber.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4640808&req=5

pone.0142439.g004: IR scanning spectra of the pigment extracted from the adductor muscle scar of Pacific oyster.‘T’ denotes the transmittance, ‘Wav’ the wavenumber.
Mentions: The IR spectrum of the pigment extracted from the adductor muscle scar of C. gigas is shown in Fig 4. The characteristic absorption peaks of the pigment were mainly distributed in the following three groups: 3500~3300 cm-1, 1620~1600 cm-1, and 1150~1000 cm-1.

Bottom Line: The maximum absorption of the pigment in the UV absorption spectrum within the range of 190-500 nm was observed between 210-220 nm.The UV absorbance decreased with increasing wavelength which was consistent with the UV spectral absorption characteristics of melanin.In addition, Fourier transform infrared spectroscopy scanning revealed characteristic absorption peaks that emerged near 3440 cm-1 and 1630 cm-1, which was consistent with infrared scanning features of eumelanin (a type of melanin).

View Article: PubMed Central - PubMed

Affiliation: School of Agriculture, Ludong University, Yantai, Shandong, China.

ABSTRACT
In this study, UV (ultraviolet) and IR (infrared radiation) spectral analysis were integrated to identify the pigment in the adductor muscle scar of the Pacific oyster Crassostrea gigas. The pigment was extracted from the adductor muscle scars of cleaned oyster shells that were pulverized, hydrolyzed in hot hydrochloric acid, purified with diethyl ether, and dissolved in 0.01 mL/L NaOH. The maximum absorption of the pigment in the UV absorption spectrum within the range of 190-500 nm was observed between 210-220 nm. The UV absorbance decreased with increasing wavelength which was consistent with the UV spectral absorption characteristics of melanin. In addition, Fourier transform infrared spectroscopy scanning revealed characteristic absorption peaks that emerged near 3440 cm-1 and 1630 cm-1, which was consistent with infrared scanning features of eumelanin (a type of melanin). This study has demonstrated for the first time that the pigment in the adductor muscle scar of the Pacific oyster is melanin, hinting that the adductor muscle could be another organ pigmenting the mollusc shell with melanin other than mantle.

No MeSH data available.


Related in: MedlinePlus