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GsCML27, a Gene Encoding a Calcium-Binding Ef-Hand Protein from Glycine soja, Plays Differential Roles in Plant Responses to Bicarbonate, Salt and Osmotic Stresses.

Chen C, Sun X, Duanmu H, Zhu D, Yu Y, Cao L, Liu A, Jia B, Xiao J, Zhu Y - PLoS ONE (2015)

Bottom Line: We further found that expression of GsCML27 was induced by bicarbonate, salt and osmotic stresses.Moreover, we also showed that GsCML27 localized in the whole cell, including cytoplasm, plasma membrane and nucleus in Arabidopsis protoplasts and onion epidermal cells, and displayed high expression in roots and embryos.Together, these data present evidence that GsCML27 as a Ca2+-binding EF-hand protein plays a role in plant responses to bicarbonate, salt and osmotic stresses.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, P.R. China.

ABSTRACT
Calcium, as the most widely accepted messenger, plays an important role in plant stress responses through calcium-dependent signaling pathways. The calmodulin-like family genes (CMLs) encode Ca2+ sensors and function in signaling transduction in response to environmental stimuli. However, until now, the function of plant CML proteins, especially soybean CMLs, is largely unknown. Here, we isolated a Glycine soja CML protein GsCML27, with four conserved EF-hands domains, and identified it as a calcium-binding protein through far-UV CD spectroscopy. We further found that expression of GsCML27 was induced by bicarbonate, salt and osmotic stresses. Interestingly, ectopic expression of GsCML27 in Arabidopsis enhanced plant tolerance to bicarbonate stress, but decreased the salt and osmotic tolerance during the seed germination and early growth stages. Furthermore, we found that ectopic expression of GsCML27 decreases salt tolerance through modifying both the cellular ionic (Na+, K+) content and the osmotic stress regulation. GsCML27 ectopic expression also decreased the expression levels of osmotic stress-responsive genes. Moreover, we also showed that GsCML27 localized in the whole cell, including cytoplasm, plasma membrane and nucleus in Arabidopsis protoplasts and onion epidermal cells, and displayed high expression in roots and embryos. Together, these data present evidence that GsCML27 as a Ca2+-binding EF-hand protein plays a role in plant responses to bicarbonate, salt and osmotic stresses.

No MeSH data available.


Related in: MedlinePlus

Expression profiles of GsCML27 in Glycine soja.A. Tissue specific expression of GsCML27 in Glycine soja. Total RNA was extracted from root, young stem, old stem, young leaf, old leaf, flower, embryo and episperm from 5-week-old soil-grown Glycine soja, and relative expression levels were determined by quantitative RT-PCR using GADPH as an internal control. B. Total RNA was extracted from roots of 3-week-old Glycine soja seedlings treated with 50 mM NaHCO3, 200 mM NaCl (salt stress) and 350 mM mannitol (osmotic stress) treatments, respectively. Relative transcript levels were determined by quantitative RT-PCR and GAPDH were used as internal controls. The mean value from three fully independent biological repeats and three technical repeats is shown.
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pone.0141888.g003: Expression profiles of GsCML27 in Glycine soja.A. Tissue specific expression of GsCML27 in Glycine soja. Total RNA was extracted from root, young stem, old stem, young leaf, old leaf, flower, embryo and episperm from 5-week-old soil-grown Glycine soja, and relative expression levels were determined by quantitative RT-PCR using GADPH as an internal control. B. Total RNA was extracted from roots of 3-week-old Glycine soja seedlings treated with 50 mM NaHCO3, 200 mM NaCl (salt stress) and 350 mM mannitol (osmotic stress) treatments, respectively. Relative transcript levels were determined by quantitative RT-PCR and GAPDH were used as internal controls. The mean value from three fully independent biological repeats and three technical repeats is shown.

Mentions: To characterize the spatial expression pattern of GsCML27 in Glycine soja, the expression levels of GsCML27 in different tissues were detected by quantitative RT-PCR analysis. The results showed that GsCML27 was expressed in most of the tissues in this study. The highest expression of GsCML27 was observed in roots and embryos, and little expression was obtained in old stems (Fig 3A), indicating tissue specificity of GsCML27 expression in Glycine soja.


GsCML27, a Gene Encoding a Calcium-Binding Ef-Hand Protein from Glycine soja, Plays Differential Roles in Plant Responses to Bicarbonate, Salt and Osmotic Stresses.

Chen C, Sun X, Duanmu H, Zhu D, Yu Y, Cao L, Liu A, Jia B, Xiao J, Zhu Y - PLoS ONE (2015)

Expression profiles of GsCML27 in Glycine soja.A. Tissue specific expression of GsCML27 in Glycine soja. Total RNA was extracted from root, young stem, old stem, young leaf, old leaf, flower, embryo and episperm from 5-week-old soil-grown Glycine soja, and relative expression levels were determined by quantitative RT-PCR using GADPH as an internal control. B. Total RNA was extracted from roots of 3-week-old Glycine soja seedlings treated with 50 mM NaHCO3, 200 mM NaCl (salt stress) and 350 mM mannitol (osmotic stress) treatments, respectively. Relative transcript levels were determined by quantitative RT-PCR and GAPDH were used as internal controls. The mean value from three fully independent biological repeats and three technical repeats is shown.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4638360&req=5

pone.0141888.g003: Expression profiles of GsCML27 in Glycine soja.A. Tissue specific expression of GsCML27 in Glycine soja. Total RNA was extracted from root, young stem, old stem, young leaf, old leaf, flower, embryo and episperm from 5-week-old soil-grown Glycine soja, and relative expression levels were determined by quantitative RT-PCR using GADPH as an internal control. B. Total RNA was extracted from roots of 3-week-old Glycine soja seedlings treated with 50 mM NaHCO3, 200 mM NaCl (salt stress) and 350 mM mannitol (osmotic stress) treatments, respectively. Relative transcript levels were determined by quantitative RT-PCR and GAPDH were used as internal controls. The mean value from three fully independent biological repeats and three technical repeats is shown.
Mentions: To characterize the spatial expression pattern of GsCML27 in Glycine soja, the expression levels of GsCML27 in different tissues were detected by quantitative RT-PCR analysis. The results showed that GsCML27 was expressed in most of the tissues in this study. The highest expression of GsCML27 was observed in roots and embryos, and little expression was obtained in old stems (Fig 3A), indicating tissue specificity of GsCML27 expression in Glycine soja.

Bottom Line: We further found that expression of GsCML27 was induced by bicarbonate, salt and osmotic stresses.Moreover, we also showed that GsCML27 localized in the whole cell, including cytoplasm, plasma membrane and nucleus in Arabidopsis protoplasts and onion epidermal cells, and displayed high expression in roots and embryos.Together, these data present evidence that GsCML27 as a Ca2+-binding EF-hand protein plays a role in plant responses to bicarbonate, salt and osmotic stresses.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, P.R. China.

ABSTRACT
Calcium, as the most widely accepted messenger, plays an important role in plant stress responses through calcium-dependent signaling pathways. The calmodulin-like family genes (CMLs) encode Ca2+ sensors and function in signaling transduction in response to environmental stimuli. However, until now, the function of plant CML proteins, especially soybean CMLs, is largely unknown. Here, we isolated a Glycine soja CML protein GsCML27, with four conserved EF-hands domains, and identified it as a calcium-binding protein through far-UV CD spectroscopy. We further found that expression of GsCML27 was induced by bicarbonate, salt and osmotic stresses. Interestingly, ectopic expression of GsCML27 in Arabidopsis enhanced plant tolerance to bicarbonate stress, but decreased the salt and osmotic tolerance during the seed germination and early growth stages. Furthermore, we found that ectopic expression of GsCML27 decreases salt tolerance through modifying both the cellular ionic (Na+, K+) content and the osmotic stress regulation. GsCML27 ectopic expression also decreased the expression levels of osmotic stress-responsive genes. Moreover, we also showed that GsCML27 localized in the whole cell, including cytoplasm, plasma membrane and nucleus in Arabidopsis protoplasts and onion epidermal cells, and displayed high expression in roots and embryos. Together, these data present evidence that GsCML27 as a Ca2+-binding EF-hand protein plays a role in plant responses to bicarbonate, salt and osmotic stresses.

No MeSH data available.


Related in: MedlinePlus