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miR-599 Inhibits Vascular Smooth Muscle Cells Proliferation and Migration by Targeting TGFB2.

Xie B, Zhang C, Kang K, Jiang S - PLoS ONE (2015)

Bottom Line: Furthermore, we identified TGFb2 as a direct target gene of miR-599 in VSMCs.Overexpression of TGFb2 reversed miR-599-induced inhibition of VSMCs proliferation and type I collagen, type V collagen and proteoglycan expression.In conclusion, our findings suggest miR-599 plays a crucial role in controlling VSMCs proliferation and matrix gene expression by regulating TGFb2 expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiovascular Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.

ABSTRACT
Aberrant proliferation and migration of vascular smooth muscle cells (VSMCs) play a crucial role in the pathogenesis of cardiovascular diseases including coronary heart disease, restenosis and atherosclerosis. MicroRNAs are a class of small, non-coding and endogenous RNAs that play critical roles in VSMCs function. In this study, we showed that PDGF-bb, as a stimulant, promoted VSMCs proliferation and suppressed the expression of miR-599. Moreover, overexpression of miR-599 inhibited VSMCs proliferation and also suppressed the PCNA and ki-67 expression. In addition, we demonstrated that ectopic expression of miR-599 repressed the VSMCs migration. We also showed that miR-599 inhibited type I collagen, type V collagen and proteoglycan expression. Furthermore, we identified TGFb2 as a direct target gene of miR-599 in VSMCs. Overexpression of TGFb2 reversed miR-599-induced inhibition of VSMCs proliferation and type I collagen, type V collagen and proteoglycan expression. In conclusion, our findings suggest miR-599 plays a crucial role in controlling VSMCs proliferation and matrix gene expression by regulating TGFb2 expression.

No MeSH data available.


Related in: MedlinePlus

miR-599 suppressed VSMCs migration.Overexpression of miR-599 suppressed VSMCs migration. ***p<0.001.
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pone.0141512.g003: miR-599 suppressed VSMCs migration.Overexpression of miR-599 suppressed VSMCs migration. ***p<0.001.

Mentions: As shown in the Fig 2A, the expression of miR-599 was increased in VSMCs after tranfection of miR-599 mimic. Ectopic expression of miR-599 suppressed the VSMCs proliferation (Fig 2B). We also found that overexpression of miR-599 inhibited the mRNA and protein expression of PCNA in VSMCs (Fig 2C and 2D). Moreover, ectopic expression of miR-599 suppressed the mRNA and protein expression of ki-67 in VSMCs (Fig 2E and 2F) (S1 Fig). In addition, we showed that ectopic expression of miR-599 inhibited VSMCs migration (Fig 3).


miR-599 Inhibits Vascular Smooth Muscle Cells Proliferation and Migration by Targeting TGFB2.

Xie B, Zhang C, Kang K, Jiang S - PLoS ONE (2015)

miR-599 suppressed VSMCs migration.Overexpression of miR-599 suppressed VSMCs migration. ***p<0.001.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4638351&req=5

pone.0141512.g003: miR-599 suppressed VSMCs migration.Overexpression of miR-599 suppressed VSMCs migration. ***p<0.001.
Mentions: As shown in the Fig 2A, the expression of miR-599 was increased in VSMCs after tranfection of miR-599 mimic. Ectopic expression of miR-599 suppressed the VSMCs proliferation (Fig 2B). We also found that overexpression of miR-599 inhibited the mRNA and protein expression of PCNA in VSMCs (Fig 2C and 2D). Moreover, ectopic expression of miR-599 suppressed the mRNA and protein expression of ki-67 in VSMCs (Fig 2E and 2F) (S1 Fig). In addition, we showed that ectopic expression of miR-599 inhibited VSMCs migration (Fig 3).

Bottom Line: Furthermore, we identified TGFb2 as a direct target gene of miR-599 in VSMCs.Overexpression of TGFb2 reversed miR-599-induced inhibition of VSMCs proliferation and type I collagen, type V collagen and proteoglycan expression.In conclusion, our findings suggest miR-599 plays a crucial role in controlling VSMCs proliferation and matrix gene expression by regulating TGFb2 expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiovascular Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.

ABSTRACT
Aberrant proliferation and migration of vascular smooth muscle cells (VSMCs) play a crucial role in the pathogenesis of cardiovascular diseases including coronary heart disease, restenosis and atherosclerosis. MicroRNAs are a class of small, non-coding and endogenous RNAs that play critical roles in VSMCs function. In this study, we showed that PDGF-bb, as a stimulant, promoted VSMCs proliferation and suppressed the expression of miR-599. Moreover, overexpression of miR-599 inhibited VSMCs proliferation and also suppressed the PCNA and ki-67 expression. In addition, we demonstrated that ectopic expression of miR-599 repressed the VSMCs migration. We also showed that miR-599 inhibited type I collagen, type V collagen and proteoglycan expression. Furthermore, we identified TGFb2 as a direct target gene of miR-599 in VSMCs. Overexpression of TGFb2 reversed miR-599-induced inhibition of VSMCs proliferation and type I collagen, type V collagen and proteoglycan expression. In conclusion, our findings suggest miR-599 plays a crucial role in controlling VSMCs proliferation and matrix gene expression by regulating TGFb2 expression.

No MeSH data available.


Related in: MedlinePlus