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Species-Specific Expression of Full-Length and Alternatively Spliced Variant Forms of CDK5RAP2.

Park JS, Lee MK, Kang S, Jin Y, Fu S, Rosales JL, Lee KY - PLoS ONE (2015)

Bottom Line: CDK5RAP2 is one of the primary microcephaly genes that are associated with reduced brain size and mental retardation.We have previously shown that human CDK5RAP2 exists as a full-length form (hCDK5RAP2) or an alternatively spliced variant form (hCDK5RAP2-V1) that is lacking exon 32.Here, we demonstrate that rat expresses both a full length and an alternatively spliced variant form of CDK5RAP2 that are equivalent to our previously reported hCDK5RAP2 and hCDK5RAP2-V1, repectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology and Anatomy, Southern Alberta Cancer Research and Hotchkiss Brain Institutes, University of Calgary, Calgary, Alberta, Canada.

ABSTRACT
CDK5RAP2 is one of the primary microcephaly genes that are associated with reduced brain size and mental retardation. We have previously shown that human CDK5RAP2 exists as a full-length form (hCDK5RAP2) or an alternatively spliced variant form (hCDK5RAP2-V1) that is lacking exon 32. The equivalent of hCDK5RAP2-V1 has been reported in rat and mouse but the presence of full-length equivalent hCDK5RAP2 in rat and mouse has not been examined. Here, we demonstrate that rat expresses both a full length and an alternatively spliced variant form of CDK5RAP2 that are equivalent to our previously reported hCDK5RAP2 and hCDK5RAP2-V1, repectively. However, mouse expresses only one form of CDK5RAP2 that is equivalent to the human and rat alternatively spliced variant forms. Knowledge of this expression of different forms of CDK5RAP2 in human, rat and mouse is essential in selecting the appropriate model for studies of CDK5RAP2 and primary microcephaly but our findings further indicate the evolutionary divergence of mouse from the human and rat species.

No MeSH data available.


Related in: MedlinePlus

A. Expression analysis of rat cdk5RAP2. Tissue distribution of rcdk5RAP2 and rcdk5RAP2-v1 transcripts as determined by RT-PCR.Rat tissue cDNAs were used as templates. No template was used for the negative control. The bottom panels show the levels of rcdk5RAP2 and rcdk5RAP2-v1 transcripts as measured by densitometry using the NIH Image J software. Data represent one of three independent experiments (n = 3). B. Expression analysis of mouse cdk5RAP2. Tissue distribution of mCDk5rap2 transcripts as determined by RT-PCR. Mouse tissue cDNAs were used as templates. No template was used for the negative control. The bottom panels show the levels of mCDk5rap2 (right panel) transcripts as measured by densitometry using the NIH Image J software. Data represent one of three independent experiments (n = 3).
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pone.0142577.g002: A. Expression analysis of rat cdk5RAP2. Tissue distribution of rcdk5RAP2 and rcdk5RAP2-v1 transcripts as determined by RT-PCR.Rat tissue cDNAs were used as templates. No template was used for the negative control. The bottom panels show the levels of rcdk5RAP2 and rcdk5RAP2-v1 transcripts as measured by densitometry using the NIH Image J software. Data represent one of three independent experiments (n = 3). B. Expression analysis of mouse cdk5RAP2. Tissue distribution of mCDk5rap2 transcripts as determined by RT-PCR. Mouse tissue cDNAs were used as templates. No template was used for the negative control. The bottom panels show the levels of mCDk5rap2 (right panel) transcripts as measured by densitometry using the NIH Image J software. Data represent one of three independent experiments (n = 3).

Mentions: We therefore investigated whether rat and mouse express the full-length CDK5RAP2 forms that are equivalent to hCDK5RAP2 as well as alternatively spliced variant forms (rCDK5RAP2-V1 and mCDK5RAP2-V1) that are equivalent to hCDK5RAP2-V1. As shown in Fig 2A, the presence of both full-length rCDK5RAP2 and alternatively spliced rCDK5RAP2-V1 transcripts, corresponding to 352 bp and 103 bp PCR fragments, respectively, was analyzed in various rat tissues by RT-PCR analysis using forward and reverse primers (see Materials and Method section) spanning human exon 32-like region.


Species-Specific Expression of Full-Length and Alternatively Spliced Variant Forms of CDK5RAP2.

Park JS, Lee MK, Kang S, Jin Y, Fu S, Rosales JL, Lee KY - PLoS ONE (2015)

A. Expression analysis of rat cdk5RAP2. Tissue distribution of rcdk5RAP2 and rcdk5RAP2-v1 transcripts as determined by RT-PCR.Rat tissue cDNAs were used as templates. No template was used for the negative control. The bottom panels show the levels of rcdk5RAP2 and rcdk5RAP2-v1 transcripts as measured by densitometry using the NIH Image J software. Data represent one of three independent experiments (n = 3). B. Expression analysis of mouse cdk5RAP2. Tissue distribution of mCDk5rap2 transcripts as determined by RT-PCR. Mouse tissue cDNAs were used as templates. No template was used for the negative control. The bottom panels show the levels of mCDk5rap2 (right panel) transcripts as measured by densitometry using the NIH Image J software. Data represent one of three independent experiments (n = 3).
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pone.0142577.g002: A. Expression analysis of rat cdk5RAP2. Tissue distribution of rcdk5RAP2 and rcdk5RAP2-v1 transcripts as determined by RT-PCR.Rat tissue cDNAs were used as templates. No template was used for the negative control. The bottom panels show the levels of rcdk5RAP2 and rcdk5RAP2-v1 transcripts as measured by densitometry using the NIH Image J software. Data represent one of three independent experiments (n = 3). B. Expression analysis of mouse cdk5RAP2. Tissue distribution of mCDk5rap2 transcripts as determined by RT-PCR. Mouse tissue cDNAs were used as templates. No template was used for the negative control. The bottom panels show the levels of mCDk5rap2 (right panel) transcripts as measured by densitometry using the NIH Image J software. Data represent one of three independent experiments (n = 3).
Mentions: We therefore investigated whether rat and mouse express the full-length CDK5RAP2 forms that are equivalent to hCDK5RAP2 as well as alternatively spliced variant forms (rCDK5RAP2-V1 and mCDK5RAP2-V1) that are equivalent to hCDK5RAP2-V1. As shown in Fig 2A, the presence of both full-length rCDK5RAP2 and alternatively spliced rCDK5RAP2-V1 transcripts, corresponding to 352 bp and 103 bp PCR fragments, respectively, was analyzed in various rat tissues by RT-PCR analysis using forward and reverse primers (see Materials and Method section) spanning human exon 32-like region.

Bottom Line: CDK5RAP2 is one of the primary microcephaly genes that are associated with reduced brain size and mental retardation.We have previously shown that human CDK5RAP2 exists as a full-length form (hCDK5RAP2) or an alternatively spliced variant form (hCDK5RAP2-V1) that is lacking exon 32.Here, we demonstrate that rat expresses both a full length and an alternatively spliced variant form of CDK5RAP2 that are equivalent to our previously reported hCDK5RAP2 and hCDK5RAP2-V1, repectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology and Anatomy, Southern Alberta Cancer Research and Hotchkiss Brain Institutes, University of Calgary, Calgary, Alberta, Canada.

ABSTRACT
CDK5RAP2 is one of the primary microcephaly genes that are associated with reduced brain size and mental retardation. We have previously shown that human CDK5RAP2 exists as a full-length form (hCDK5RAP2) or an alternatively spliced variant form (hCDK5RAP2-V1) that is lacking exon 32. The equivalent of hCDK5RAP2-V1 has been reported in rat and mouse but the presence of full-length equivalent hCDK5RAP2 in rat and mouse has not been examined. Here, we demonstrate that rat expresses both a full length and an alternatively spliced variant form of CDK5RAP2 that are equivalent to our previously reported hCDK5RAP2 and hCDK5RAP2-V1, repectively. However, mouse expresses only one form of CDK5RAP2 that is equivalent to the human and rat alternatively spliced variant forms. Knowledge of this expression of different forms of CDK5RAP2 in human, rat and mouse is essential in selecting the appropriate model for studies of CDK5RAP2 and primary microcephaly but our findings further indicate the evolutionary divergence of mouse from the human and rat species.

No MeSH data available.


Related in: MedlinePlus