Limits...
Effects of papaverine on carbachol- and high K(+)-induced contraction in the bovine abomasum.

Kaneda T, Saito E, Kanda H, Urakawa N, Shimizu K - J. Vet. Med. Sci. (2015)

Bottom Line: Changes in the extracellular Ca(2+) from 1.5 mM to 7.5 mM reduced verapamil-induced relaxation in high K(+)-depolarized muscles, but papaverine-induced relaxation did not change.Futhermore, papaverine (30 µM) and NaCN (300 µM) decreased the creatine phosphate contents.These results suggest that the relaxing effects of papaverine on the bovine abomasum are mainly due to the inhibition of aerobic energy metabolism.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Nippon Veterinary and Life Science University, 7-1 Kyonan-cho 1-chome, Musashino, Tokyo 180-8602, Japan.

ABSTRACT
The effects of papaverine on carbachol (CCh) -and high K(+)- induced contraction in the bovine abomasum were investigated. Papaverine inhibited CCh (1 µM) -and KCl (65 mM) -induced contractions in a concentration-dependent manner. Forskolin or sodium nitroprusside inhibited CCh-induced contractions in a concentration-dependent manner in association with increases in the cAMP or cGMP contents, whereas papaverine increased cGMP contents only at 30 µM. Changes in the extracellular Ca(2+) from 1.5 mM to 7.5 mM reduced verapamil-induced relaxation in high K(+)-depolarized muscles, but papaverine-induced relaxation did not change. Futhermore, papaverine (30 µM) and NaCN (300 µM) decreased the creatine phosphate contents. These results suggest that the relaxing effects of papaverine on the bovine abomasum are mainly due to the inhibition of aerobic energy metabolism.

No MeSH data available.


Related in: MedlinePlus

Effects of papaverine, forskolin and sodium nitroprusside (SNP) on the contractioninduced by 1 µM carbachol (CCh) and 65 mM KCl (high K+) inbovine abomasum. Preparations were precontracted with CCh or high K+, andthen, the specified agents were added to the bath solution. The maximum contractionsinduced by high K+ and CCh in the absence of these agents were taken as100%. Each point represents the mean of 4–7 preparations. Vertical bars indicateSEM.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4638294&req=5

fig_001: Effects of papaverine, forskolin and sodium nitroprusside (SNP) on the contractioninduced by 1 µM carbachol (CCh) and 65 mM KCl (high K+) inbovine abomasum. Preparations were precontracted with CCh or high K+, andthen, the specified agents were added to the bath solution. The maximum contractionsinduced by high K+ and CCh in the absence of these agents were taken as100%. Each point represents the mean of 4–7 preparations. Vertical bars indicateSEM.

Mentions: Effects of papaverine, forskolin or sodium nitroprusside on the CCh- or highK+-induced contraction: When a contractile response induced by 65 mMKCl (high K+) or 1 µM CCh reached a steady level about 15–20 minafter application, papaverine (1–100 µM) was added cumulatively. Papaverineinhibited the CCh- or high K+-induced contraction in a concentration-dependentmanner (Fig. 1AFig. 1.


Effects of papaverine on carbachol- and high K(+)-induced contraction in the bovine abomasum.

Kaneda T, Saito E, Kanda H, Urakawa N, Shimizu K - J. Vet. Med. Sci. (2015)

Effects of papaverine, forskolin and sodium nitroprusside (SNP) on the contractioninduced by 1 µM carbachol (CCh) and 65 mM KCl (high K+) inbovine abomasum. Preparations were precontracted with CCh or high K+, andthen, the specified agents were added to the bath solution. The maximum contractionsinduced by high K+ and CCh in the absence of these agents were taken as100%. Each point represents the mean of 4–7 preparations. Vertical bars indicateSEM.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4638294&req=5

fig_001: Effects of papaverine, forskolin and sodium nitroprusside (SNP) on the contractioninduced by 1 µM carbachol (CCh) and 65 mM KCl (high K+) inbovine abomasum. Preparations were precontracted with CCh or high K+, andthen, the specified agents were added to the bath solution. The maximum contractionsinduced by high K+ and CCh in the absence of these agents were taken as100%. Each point represents the mean of 4–7 preparations. Vertical bars indicateSEM.
Mentions: Effects of papaverine, forskolin or sodium nitroprusside on the CCh- or highK+-induced contraction: When a contractile response induced by 65 mMKCl (high K+) or 1 µM CCh reached a steady level about 15–20 minafter application, papaverine (1–100 µM) was added cumulatively. Papaverineinhibited the CCh- or high K+-induced contraction in a concentration-dependentmanner (Fig. 1AFig. 1.

Bottom Line: Changes in the extracellular Ca(2+) from 1.5 mM to 7.5 mM reduced verapamil-induced relaxation in high K(+)-depolarized muscles, but papaverine-induced relaxation did not change.Futhermore, papaverine (30 µM) and NaCN (300 µM) decreased the creatine phosphate contents.These results suggest that the relaxing effects of papaverine on the bovine abomasum are mainly due to the inhibition of aerobic energy metabolism.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Nippon Veterinary and Life Science University, 7-1 Kyonan-cho 1-chome, Musashino, Tokyo 180-8602, Japan.

ABSTRACT
The effects of papaverine on carbachol (CCh) -and high K(+)- induced contraction in the bovine abomasum were investigated. Papaverine inhibited CCh (1 µM) -and KCl (65 mM) -induced contractions in a concentration-dependent manner. Forskolin or sodium nitroprusside inhibited CCh-induced contractions in a concentration-dependent manner in association with increases in the cAMP or cGMP contents, whereas papaverine increased cGMP contents only at 30 µM. Changes in the extracellular Ca(2+) from 1.5 mM to 7.5 mM reduced verapamil-induced relaxation in high K(+)-depolarized muscles, but papaverine-induced relaxation did not change. Futhermore, papaverine (30 µM) and NaCN (300 µM) decreased the creatine phosphate contents. These results suggest that the relaxing effects of papaverine on the bovine abomasum are mainly due to the inhibition of aerobic energy metabolism.

No MeSH data available.


Related in: MedlinePlus