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The combined effect of clothianidin and environmental stress on the behavioral and reproductive function in male mice.

Hirano T, Yanai S, Omotehara T, Hashimoto R, Umemura Y, Kubota N, Minami K, Nagahara D, Matsuo E, Aihara Y, Shinohara R, Furuyashiki T, Mantani Y, Yokoyama T, Kitagawa H, Hoshi N - J. Vet. Med. Sci. (2015)

Bottom Line: Mammal nAChRs appear to have a certain affinity for neonicotinoids with lower levels than those of insects; there is thus rising concern about unpredictable adverse effects of neonicotinoids on vertebrates.We hypothesized that the effects of neonicotinoids would be enhanced under a chronic stressed condition, which is known to alter the expression of targets of neonicotinoids, i.e., neuronal nAChRs.The present study demonstrates that the behavioral and reproductive effects of CTD become more serious in combination with environmental stress, which may reflect our actual situation of multiple exposure.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Molecular Morphology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo 657-8501, Japan.

ABSTRACT
Neonicotinoids, some of the most widely used pesticides in the world, act as agonists to the nicotinic acetylcholine receptors (nAChRs) of insects, resulting in death from abnormal excitability. Neonicotinoids unexpectedly became a major topic as a compelling cause of honeybee colony collapse disorder, which is damaging crop production that requires pollination worldwide. Mammal nAChRs appear to have a certain affinity for neonicotinoids with lower levels than those of insects; there is thus rising concern about unpredictable adverse effects of neonicotinoids on vertebrates. We hypothesized that the effects of neonicotinoids would be enhanced under a chronic stressed condition, which is known to alter the expression of targets of neonicotinoids, i.e., neuronal nAChRs. We performed immunohistochemical and behavioral analyses in male mice actively administered a neonicotinoid, clothianidin (CTD; 0, 10, 50 and 250 mg/kg/day), for 4 weeks under an unpredictable chronic stress procedure. Vacuolated seminiferous epithelia and a decrease in the immunoreactivity of the antioxidant enzyme glutathione peroxidase 4 were observed in the testes of the CTD+stress mice. In an open field test, although the locomotor activities were not affected, the anxiety-like behaviors of the mice were elevated by both CTD and stress. The present study demonstrates that the behavioral and reproductive effects of CTD become more serious in combination with environmental stress, which may reflect our actual situation of multiple exposure.

No MeSH data available.


Related in: MedlinePlus

Representative histology of the testis in the non-stressed and stressed groups. Theseminiferous tubules of the control group showed robust spermatogenesis with denselystacked germ cells. Multinucleated giant cells (arrow) were occasionally observed inthe testes of the CTD-0+stress groups. In the CTD groups, dose-dependent vacuoleddegeneration (arrowhead) of seminiferous tubules was observed. Degeneratedseminiferous tubules only composed of Sertoli cells were present in the CTD-250groups. Bar=100 µm.
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fig_003: Representative histology of the testis in the non-stressed and stressed groups. Theseminiferous tubules of the control group showed robust spermatogenesis with denselystacked germ cells. Multinucleated giant cells (arrow) were occasionally observed inthe testes of the CTD-0+stress groups. In the CTD groups, dose-dependent vacuoleddegeneration (arrowhead) of seminiferous tubules was observed. Degeneratedseminiferous tubules only composed of Sertoli cells were present in the CTD-250groups. Bar=100 µm.

Mentions: Histological and immunohistochemical findings: The general histologicalanalyses of the testes by HE staining are shown in Fig.3Fig. 3.


The combined effect of clothianidin and environmental stress on the behavioral and reproductive function in male mice.

Hirano T, Yanai S, Omotehara T, Hashimoto R, Umemura Y, Kubota N, Minami K, Nagahara D, Matsuo E, Aihara Y, Shinohara R, Furuyashiki T, Mantani Y, Yokoyama T, Kitagawa H, Hoshi N - J. Vet. Med. Sci. (2015)

Representative histology of the testis in the non-stressed and stressed groups. Theseminiferous tubules of the control group showed robust spermatogenesis with denselystacked germ cells. Multinucleated giant cells (arrow) were occasionally observed inthe testes of the CTD-0+stress groups. In the CTD groups, dose-dependent vacuoleddegeneration (arrowhead) of seminiferous tubules was observed. Degeneratedseminiferous tubules only composed of Sertoli cells were present in the CTD-250groups. Bar=100 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4638285&req=5

fig_003: Representative histology of the testis in the non-stressed and stressed groups. Theseminiferous tubules of the control group showed robust spermatogenesis with denselystacked germ cells. Multinucleated giant cells (arrow) were occasionally observed inthe testes of the CTD-0+stress groups. In the CTD groups, dose-dependent vacuoleddegeneration (arrowhead) of seminiferous tubules was observed. Degeneratedseminiferous tubules only composed of Sertoli cells were present in the CTD-250groups. Bar=100 µm.
Mentions: Histological and immunohistochemical findings: The general histologicalanalyses of the testes by HE staining are shown in Fig.3Fig. 3.

Bottom Line: Mammal nAChRs appear to have a certain affinity for neonicotinoids with lower levels than those of insects; there is thus rising concern about unpredictable adverse effects of neonicotinoids on vertebrates.We hypothesized that the effects of neonicotinoids would be enhanced under a chronic stressed condition, which is known to alter the expression of targets of neonicotinoids, i.e., neuronal nAChRs.The present study demonstrates that the behavioral and reproductive effects of CTD become more serious in combination with environmental stress, which may reflect our actual situation of multiple exposure.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Molecular Morphology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo 657-8501, Japan.

ABSTRACT
Neonicotinoids, some of the most widely used pesticides in the world, act as agonists to the nicotinic acetylcholine receptors (nAChRs) of insects, resulting in death from abnormal excitability. Neonicotinoids unexpectedly became a major topic as a compelling cause of honeybee colony collapse disorder, which is damaging crop production that requires pollination worldwide. Mammal nAChRs appear to have a certain affinity for neonicotinoids with lower levels than those of insects; there is thus rising concern about unpredictable adverse effects of neonicotinoids on vertebrates. We hypothesized that the effects of neonicotinoids would be enhanced under a chronic stressed condition, which is known to alter the expression of targets of neonicotinoids, i.e., neuronal nAChRs. We performed immunohistochemical and behavioral analyses in male mice actively administered a neonicotinoid, clothianidin (CTD; 0, 10, 50 and 250 mg/kg/day), for 4 weeks under an unpredictable chronic stress procedure. Vacuolated seminiferous epithelia and a decrease in the immunoreactivity of the antioxidant enzyme glutathione peroxidase 4 were observed in the testes of the CTD+stress mice. In an open field test, although the locomotor activities were not affected, the anxiety-like behaviors of the mice were elevated by both CTD and stress. The present study demonstrates that the behavioral and reproductive effects of CTD become more serious in combination with environmental stress, which may reflect our actual situation of multiple exposure.

No MeSH data available.


Related in: MedlinePlus