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Immunosuppressive phenolic compounds from Hydnora abyssinica A. Braun.

Koko WS, Mesaik MA, Ranjitt R, Galal M, Choudhary MI - BMC Complement Altern Med (2015)

Bottom Line: Catechin, (1), tyrosol (2) and benzoic acid, 3, 4, dihydroxy-, ethyl ester (3) compounds were isolated from HA ethanolic extract which revealed potent immunosuppressive activity against reactive oxygen species from both polymorph nuclear cells (PMNs) (45-90 % inhibition) and mononuclear cells (MNCs) (30 -65 % inhibition), T lymphocyte proliferation assay (70-93 % inhibition) as well as potent inhibitory effect against superoxide production (42-71 % inhibition) at concentrations of 6.25-100 μg/mL.Catechin (1) was found the most potent immunosuppressive agent among all constituents examined.These results can support the traditional uses of H. abyssinica extracts as anti-inflammatory and immunosuppressive and further investigations of the mode of action and other pharmacological studies are highly desirable.

View Article: PubMed Central - PubMed

Affiliation: College of Science and Arts in Ar Rass, University of Qassim, Qassim, KSA, Saudi Arabia. wasyko2002@yahoo.com.

ABSTRACT

Background: Hydnora abyssinica (HA) A. Braun is an endemic Sudanese medicinal plant traditionally used as anti-inflammatory and against many infectious diseases. However, it proved to be very rich in phenols and tannins, so the present study was undertaken to investigate the immunomodulatory potential of the whole plant ethanolic extract and its isolated compounds.

Methods: Lymphocyte proliferation, chemiluminescence and superoxide reduction assays were used for immunomodulatory evaluation. While, MTT (3-(4, 5-dimethylthazol-2-yl)-2, 5-diphenyl tetrazonium bromide) test was performed on 3 T3 cell line clone in order to evaluate the cytoxicity effect of the extracts and isolated compounds of phenolic derivatives which were carried out by chromotographic techniques.

Results: Catechin, (1), tyrosol (2) and benzoic acid, 3, 4, dihydroxy-, ethyl ester (3) compounds were isolated from HA ethanolic extract which revealed potent immunosuppressive activity against reactive oxygen species from both polymorph nuclear cells (PMNs) (45-90 % inhibition) and mononuclear cells (MNCs) (30 -65 % inhibition), T lymphocyte proliferation assay (70-93 % inhibition) as well as potent inhibitory effect against superoxide production (42-71 % inhibition) at concentrations of 6.25-100 μg/mL. Catechin (1) was found the most potent immunosuppressive agent among all constituents examined.

Conclusion: These results can support the traditional uses of H. abyssinica extracts as anti-inflammatory and immunosuppressive and further investigations of the mode of action and other pharmacological studies are highly desirable.

No MeSH data available.


Related in: MedlinePlus

Inhibitory properties of isolated phenolic compounds for PMNs activated by SOZ (luminol based)
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Fig3: Inhibitory properties of isolated phenolic compounds for PMNs activated by SOZ (luminol based)

Mentions: The preliminary screening results of H. abyssinica ethanolic extract on both isolated immune cells types,PMNs and MNCs was carried out after activation of cells using serum opsonized zymosan (SOZ) and followed by luminol as indicator. The dose dependent inhibitory properties for phagocytosis was reported for all tested concentrations (6.25 to 100 μg/mL) against both PMNs (45–90 % inhibition) and MNCs (30–65 % inhibition) Fig. 2. The inhibitory effects of the isolated compounds on PMNs activated by SOZ was found 92, 79 and 95 % with higher concentration {50 μg/mL} and 30, 25 and 5 % with the lower concentration {3.15 μg/mL} for the compounds catechin (0.17–0.01 mM), tyrosol (0.36–0.02 mM) and benzoic acid derivative (0.27–0.02 mM), respectively Fig. 3.Fig. 2


Immunosuppressive phenolic compounds from Hydnora abyssinica A. Braun.

Koko WS, Mesaik MA, Ranjitt R, Galal M, Choudhary MI - BMC Complement Altern Med (2015)

Inhibitory properties of isolated phenolic compounds for PMNs activated by SOZ (luminol based)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4638089&req=5

Fig3: Inhibitory properties of isolated phenolic compounds for PMNs activated by SOZ (luminol based)
Mentions: The preliminary screening results of H. abyssinica ethanolic extract on both isolated immune cells types,PMNs and MNCs was carried out after activation of cells using serum opsonized zymosan (SOZ) and followed by luminol as indicator. The dose dependent inhibitory properties for phagocytosis was reported for all tested concentrations (6.25 to 100 μg/mL) against both PMNs (45–90 % inhibition) and MNCs (30–65 % inhibition) Fig. 2. The inhibitory effects of the isolated compounds on PMNs activated by SOZ was found 92, 79 and 95 % with higher concentration {50 μg/mL} and 30, 25 and 5 % with the lower concentration {3.15 μg/mL} for the compounds catechin (0.17–0.01 mM), tyrosol (0.36–0.02 mM) and benzoic acid derivative (0.27–0.02 mM), respectively Fig. 3.Fig. 2

Bottom Line: Catechin, (1), tyrosol (2) and benzoic acid, 3, 4, dihydroxy-, ethyl ester (3) compounds were isolated from HA ethanolic extract which revealed potent immunosuppressive activity against reactive oxygen species from both polymorph nuclear cells (PMNs) (45-90 % inhibition) and mononuclear cells (MNCs) (30 -65 % inhibition), T lymphocyte proliferation assay (70-93 % inhibition) as well as potent inhibitory effect against superoxide production (42-71 % inhibition) at concentrations of 6.25-100 μg/mL.Catechin (1) was found the most potent immunosuppressive agent among all constituents examined.These results can support the traditional uses of H. abyssinica extracts as anti-inflammatory and immunosuppressive and further investigations of the mode of action and other pharmacological studies are highly desirable.

View Article: PubMed Central - PubMed

Affiliation: College of Science and Arts in Ar Rass, University of Qassim, Qassim, KSA, Saudi Arabia. wasyko2002@yahoo.com.

ABSTRACT

Background: Hydnora abyssinica (HA) A. Braun is an endemic Sudanese medicinal plant traditionally used as anti-inflammatory and against many infectious diseases. However, it proved to be very rich in phenols and tannins, so the present study was undertaken to investigate the immunomodulatory potential of the whole plant ethanolic extract and its isolated compounds.

Methods: Lymphocyte proliferation, chemiluminescence and superoxide reduction assays were used for immunomodulatory evaluation. While, MTT (3-(4, 5-dimethylthazol-2-yl)-2, 5-diphenyl tetrazonium bromide) test was performed on 3 T3 cell line clone in order to evaluate the cytoxicity effect of the extracts and isolated compounds of phenolic derivatives which were carried out by chromotographic techniques.

Results: Catechin, (1), tyrosol (2) and benzoic acid, 3, 4, dihydroxy-, ethyl ester (3) compounds were isolated from HA ethanolic extract which revealed potent immunosuppressive activity against reactive oxygen species from both polymorph nuclear cells (PMNs) (45-90 % inhibition) and mononuclear cells (MNCs) (30 -65 % inhibition), T lymphocyte proliferation assay (70-93 % inhibition) as well as potent inhibitory effect against superoxide production (42-71 % inhibition) at concentrations of 6.25-100 μg/mL. Catechin (1) was found the most potent immunosuppressive agent among all constituents examined.

Conclusion: These results can support the traditional uses of H. abyssinica extracts as anti-inflammatory and immunosuppressive and further investigations of the mode of action and other pharmacological studies are highly desirable.

No MeSH data available.


Related in: MedlinePlus