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Human papillomavirus type 16 E7 oncoprotein mediates CCNA1 promoter methylation.

Chalertpet K, Pakdeechaidan W, Patel V, Mutirangura A, Yanatatsaneejit P - Cancer Sci. (2015)

Bottom Line: CCNA1 methylation is found in HPV-associated cervical cancers, as well as in head and neck squamous cell cancer.Using both E7 knockdown and overexpression approaches in SiHa and C33a cells, our data showed that CCNA1 promoter methylation decreases with a corresponding increase in expression in E7 siRNA-transfected cells.By contrast, CCNA1 promoter methylation was augmented with a corresponding reduction in expression in E7-overexpressing cells.

View Article: PubMed Central - PubMed

Affiliation: Human Genetics Research Group, Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.

No MeSH data available.


Related in: MedlinePlus

DNA methyltransferase (Dnmt) family member expression (Dnmt1, Dnmt3a, and Dnmt3b) in E7- and empty vector (PC)-transfected cells. β-actin was used an internal control.
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fig05: DNA methyltransferase (Dnmt) family member expression (Dnmt1, Dnmt3a, and Dnmt3b) in E7- and empty vector (PC)-transfected cells. β-actin was used an internal control.

Mentions: To observe the expression of Dnmt1, Dnmt3a, and Dnmt3b in E7-transfected cells by Western blot analysis, a decreasing level of Dnmt1 protein in E7-overexpressing C33a cells was observed compared with control cells. In contrast, there was no difference between the expression of Dnmt3a and Dnmt3b in E7-overexpressing C33a and control cells (Fig. 5).


Human papillomavirus type 16 E7 oncoprotein mediates CCNA1 promoter methylation.

Chalertpet K, Pakdeechaidan W, Patel V, Mutirangura A, Yanatatsaneejit P - Cancer Sci. (2015)

DNA methyltransferase (Dnmt) family member expression (Dnmt1, Dnmt3a, and Dnmt3b) in E7- and empty vector (PC)-transfected cells. β-actin was used an internal control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4638020&req=5

fig05: DNA methyltransferase (Dnmt) family member expression (Dnmt1, Dnmt3a, and Dnmt3b) in E7- and empty vector (PC)-transfected cells. β-actin was used an internal control.
Mentions: To observe the expression of Dnmt1, Dnmt3a, and Dnmt3b in E7-transfected cells by Western blot analysis, a decreasing level of Dnmt1 protein in E7-overexpressing C33a cells was observed compared with control cells. In contrast, there was no difference between the expression of Dnmt3a and Dnmt3b in E7-overexpressing C33a and control cells (Fig. 5).

Bottom Line: CCNA1 methylation is found in HPV-associated cervical cancers, as well as in head and neck squamous cell cancer.Using both E7 knockdown and overexpression approaches in SiHa and C33a cells, our data showed that CCNA1 promoter methylation decreases with a corresponding increase in expression in E7 siRNA-transfected cells.By contrast, CCNA1 promoter methylation was augmented with a corresponding reduction in expression in E7-overexpressing cells.

View Article: PubMed Central - PubMed

Affiliation: Human Genetics Research Group, Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.

No MeSH data available.


Related in: MedlinePlus