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Seminal plasma hexosaminidase in patients with normal and abnormal spermograms.

Menendez-Helman RJ, Sanjurjo C, Miranda PV - Iran J Reprod Med (2015)

Bottom Line: Hex activity, like glucosidase, was significantly reduced in azoospermic samples (44, 49, and 60% reduction for total Hex, HexA and glucosidase, respectively).A reduced amount of Hex in azoospermic samples was confirmed by western immunoblot.The results suggested that Hex activity was reduced in azoospermic samples and this was due to a lower amount of enzyme.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Biología y Medicina Experimental - CONICET. Buenos Aires, Argentina. ; IQUIBICEN (FCEyN, UBA - CONICET). Buenos Aires, Argentina.

ABSTRACT

Background: Glycosidases profusion in male reproductive fluids suggests a possible relationship with sperm function. Although Hexosaminidase (Hex) is the most active glycosidase in epididymal fluid and seminal plasma, as well as in spermatozoa, Glucosidase is considered a marker for epididymal function and azoospermia.

Objective: The aim of this study was to determine Hex activity in seminal plasma from patients with normal and abnormal spermograms and analyze its correlation with seminal parameters.

Materials and methods: In this cross sectional study, seminal plasma from azoospermic, asthenozoospermic, teratozoospermic, and normozoospermic patients was analyzed for the activity of: total Hex, HexA isoform, and glucosidase. Besides, hexosamine levels were determined, and the amount of Hex was quantified by immunoblot with a specific antibody. Correlation of Hex activity with seminal parameters was also analyzed.

Results: Hex activity, like glucosidase, was significantly reduced in azoospermic samples (44, 49, and 60% reduction for total Hex, HexA and glucosidase, respectively). A reduced amount of Hex in azoospermic samples was confirmed by western immunoblot. Hex activity was negatively correlated with round cells in azoospermic samples and positively correlated with motility in asthenozoospermic ones.

Conclusion: The results suggested that Hex activity was reduced in azoospermic samples and this was due to a lower amount of enzyme. The correlation to seminal parameters related to particular pathologies suggests a possible relationship of Hex with fertilizing capacity.

No MeSH data available.


Related in: MedlinePlus

Standard curves for both hexosamines, N-acetyglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc), in the Morgan- Elson method.
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Figure 2: Standard curves for both hexosamines, N-acetyglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc), in the Morgan- Elson method.

Mentions: Considering that seminal vesicles secrete hexosamines which are able to inhibit Hex catalytic capacity, we analyzed if the lower Hex activity in the azoospermic group could be due to a higher concentration of hexosamines. First, the inhibitory capacity of both hexosamines on seminal plasma Hex was analyzed using samples from fertile donors. Both monosaccharides showed typical inhibition curves with EC50 values of 10 ± 1 and 0.8 ± 0.3 mM for GlcNAc and GalNAc, respectively (Figure 1). On the other hand, the concentration of hexosamines in seminal plasma from each group was determined. This quantification was currently done using a colorimetric procedure where both monosaccharides react giving a different response (Figure 2). Although the signal could not be assigned to a particular hexosamine, the average absorbance was found to be similar for all groups (Figure 3) suggesting that different levels of these monosaccharides in seminal plasma would not be the reason for the low Hex activity in the azoospermic group. To evaluate the possibility that the reduced Hex activity was the result of a lower concentration of enzyme, the amount of enzyme in seminal plasma was determined by western immunoblot.


Seminal plasma hexosaminidase in patients with normal and abnormal spermograms.

Menendez-Helman RJ, Sanjurjo C, Miranda PV - Iran J Reprod Med (2015)

Standard curves for both hexosamines, N-acetyglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc), in the Morgan- Elson method.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4637121&req=5

Figure 2: Standard curves for both hexosamines, N-acetyglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc), in the Morgan- Elson method.
Mentions: Considering that seminal vesicles secrete hexosamines which are able to inhibit Hex catalytic capacity, we analyzed if the lower Hex activity in the azoospermic group could be due to a higher concentration of hexosamines. First, the inhibitory capacity of both hexosamines on seminal plasma Hex was analyzed using samples from fertile donors. Both monosaccharides showed typical inhibition curves with EC50 values of 10 ± 1 and 0.8 ± 0.3 mM for GlcNAc and GalNAc, respectively (Figure 1). On the other hand, the concentration of hexosamines in seminal plasma from each group was determined. This quantification was currently done using a colorimetric procedure where both monosaccharides react giving a different response (Figure 2). Although the signal could not be assigned to a particular hexosamine, the average absorbance was found to be similar for all groups (Figure 3) suggesting that different levels of these monosaccharides in seminal plasma would not be the reason for the low Hex activity in the azoospermic group. To evaluate the possibility that the reduced Hex activity was the result of a lower concentration of enzyme, the amount of enzyme in seminal plasma was determined by western immunoblot.

Bottom Line: Hex activity, like glucosidase, was significantly reduced in azoospermic samples (44, 49, and 60% reduction for total Hex, HexA and glucosidase, respectively).A reduced amount of Hex in azoospermic samples was confirmed by western immunoblot.The results suggested that Hex activity was reduced in azoospermic samples and this was due to a lower amount of enzyme.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Biología y Medicina Experimental - CONICET. Buenos Aires, Argentina. ; IQUIBICEN (FCEyN, UBA - CONICET). Buenos Aires, Argentina.

ABSTRACT

Background: Glycosidases profusion in male reproductive fluids suggests a possible relationship with sperm function. Although Hexosaminidase (Hex) is the most active glycosidase in epididymal fluid and seminal plasma, as well as in spermatozoa, Glucosidase is considered a marker for epididymal function and azoospermia.

Objective: The aim of this study was to determine Hex activity in seminal plasma from patients with normal and abnormal spermograms and analyze its correlation with seminal parameters.

Materials and methods: In this cross sectional study, seminal plasma from azoospermic, asthenozoospermic, teratozoospermic, and normozoospermic patients was analyzed for the activity of: total Hex, HexA isoform, and glucosidase. Besides, hexosamine levels were determined, and the amount of Hex was quantified by immunoblot with a specific antibody. Correlation of Hex activity with seminal parameters was also analyzed.

Results: Hex activity, like glucosidase, was significantly reduced in azoospermic samples (44, 49, and 60% reduction for total Hex, HexA and glucosidase, respectively). A reduced amount of Hex in azoospermic samples was confirmed by western immunoblot. Hex activity was negatively correlated with round cells in azoospermic samples and positively correlated with motility in asthenozoospermic ones.

Conclusion: The results suggested that Hex activity was reduced in azoospermic samples and this was due to a lower amount of enzyme. The correlation to seminal parameters related to particular pathologies suggests a possible relationship of Hex with fertilizing capacity.

No MeSH data available.


Related in: MedlinePlus