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The adverse effects of the methoxsalen and ultraviolent A radiation on spermatogenesis in mice.

Farhadi M, Mohseni Kouchesfahani H, Shockravi A, Foroozanfar M, Parivar K - Iran J Reprod Med (2015)

Bottom Line: Different investigation showed that 5-methoxypsoralen and 8- methoxypsoralen reduce birth rates in the rats.Our results showed the number of spermatozoa in experimental group I was 22.6±2.12, in experimental group II was 33.6±2.05 and in control group was 44.3±2.77 (p<0.05).It is concluded from the results of this work that treatment with methoxsalen with UVA can damage and disorganize seminiferous tubules and decrease spermatogenic cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Mcrobiology, Karaj Branch, Islamic Azad University, Karaj, Iran.

ABSTRACT

Background: Different investigation showed that 5-methoxypsoralen and 8- methoxypsoralen reduce birth rates in the rats.

Objective: In this study we worked out the effect of methoxsalen together with ultraviolent A (UVA) radiation on mature Balb/C mice spermatogenesis.

Materials and methods: The LD50 standard was determined 160 mg/kg and the UVA dose which causes erythema was calculated 0.046 J/cm2. A sub-lethal dose of 80 mg/kg of methoxsalen solution was injected intrapritoneally to mature mice and after one hour they were exposed to UVA radiation for 20 minutes. Experiments applied included methoxsalen alone, methoxsalen with UVA, UVA alone, sham group (a group received Tween 80), and control group (N=6). In all experimental groups except UVA alone group, injections were carried out, during two consecutive weeks. Serial cross sections (5 µm thickness) were prepared for morphological and histological studies. Tunica albuginea diameter, and number of type A and type B spermatogonia and histological investigation of the testes were measured.

Results: Microscopical and statistical analyses showed significant anomalies among the experimental groups compared to control and sham group. These anomalies included decrease the body weight; increase the relative testis weight; and decrease the number of spermapogonia (type A and B), primary spermatocytes, spermatids and sperms in experimental groups I and II compared to control group. Our results showed the number of spermatozoa in experimental group I was 22.6±2.12, in experimental group II was 33.6±2.05 and in control group was 44.3±2.77 (p<0.05). Moreover in some experimental groups (I and II) shrinkage of seminiferous tubules and release of primary spermatocyte and spermatids were observed to the lumen of them.

Conclusion: It is concluded from the results of this work that treatment with methoxsalen with UVA can damage and disorganize seminiferous tubules and decrease spermatogenic cells.

No MeSH data available.


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Photomicrograph of seminiferous tubules of testis. Control (left) experimental: methoxsalen with UVA (right). The shrank seminiferous tubule with empty lumen of sperm (1000X
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Figure 2: Photomicrograph of seminiferous tubules of testis. Control (left) experimental: methoxsalen with UVA (right). The shrank seminiferous tubule with empty lumen of sperm (1000X

Mentions: The results showed that body weight reduced significantly in all experimental groups compared to the control group. Relative testes weight and the thickness of tunica albuginea, was significantly increased (Figure 1) in experimental groups I and II, while the testis volume and its length and diameter did not show significant changes (Table I). The number of type A and B spermatogonia, primary spermatocytes, spermatids and sperms were significantly reduced in all experimental groups, compared to control group (Table II). In addition, PUVA experience (experimental group I) and methoxsalen alone expeience (experimental group II) showed shrinkage of seminiferous tubules, release of primary spermatocytes and sperms into the lumen, and disorganized seminiferous tubules (Figure 2).


The adverse effects of the methoxsalen and ultraviolent A radiation on spermatogenesis in mice.

Farhadi M, Mohseni Kouchesfahani H, Shockravi A, Foroozanfar M, Parivar K - Iran J Reprod Med (2015)

Photomicrograph of seminiferous tubules of testis. Control (left) experimental: methoxsalen with UVA (right). The shrank seminiferous tubule with empty lumen of sperm (1000X
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4637113&req=5

Figure 2: Photomicrograph of seminiferous tubules of testis. Control (left) experimental: methoxsalen with UVA (right). The shrank seminiferous tubule with empty lumen of sperm (1000X
Mentions: The results showed that body weight reduced significantly in all experimental groups compared to the control group. Relative testes weight and the thickness of tunica albuginea, was significantly increased (Figure 1) in experimental groups I and II, while the testis volume and its length and diameter did not show significant changes (Table I). The number of type A and B spermatogonia, primary spermatocytes, spermatids and sperms were significantly reduced in all experimental groups, compared to control group (Table II). In addition, PUVA experience (experimental group I) and methoxsalen alone expeience (experimental group II) showed shrinkage of seminiferous tubules, release of primary spermatocytes and sperms into the lumen, and disorganized seminiferous tubules (Figure 2).

Bottom Line: Different investigation showed that 5-methoxypsoralen and 8- methoxypsoralen reduce birth rates in the rats.Our results showed the number of spermatozoa in experimental group I was 22.6±2.12, in experimental group II was 33.6±2.05 and in control group was 44.3±2.77 (p<0.05).It is concluded from the results of this work that treatment with methoxsalen with UVA can damage and disorganize seminiferous tubules and decrease spermatogenic cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Mcrobiology, Karaj Branch, Islamic Azad University, Karaj, Iran.

ABSTRACT

Background: Different investigation showed that 5-methoxypsoralen and 8- methoxypsoralen reduce birth rates in the rats.

Objective: In this study we worked out the effect of methoxsalen together with ultraviolent A (UVA) radiation on mature Balb/C mice spermatogenesis.

Materials and methods: The LD50 standard was determined 160 mg/kg and the UVA dose which causes erythema was calculated 0.046 J/cm2. A sub-lethal dose of 80 mg/kg of methoxsalen solution was injected intrapritoneally to mature mice and after one hour they were exposed to UVA radiation for 20 minutes. Experiments applied included methoxsalen alone, methoxsalen with UVA, UVA alone, sham group (a group received Tween 80), and control group (N=6). In all experimental groups except UVA alone group, injections were carried out, during two consecutive weeks. Serial cross sections (5 µm thickness) were prepared for morphological and histological studies. Tunica albuginea diameter, and number of type A and type B spermatogonia and histological investigation of the testes were measured.

Results: Microscopical and statistical analyses showed significant anomalies among the experimental groups compared to control and sham group. These anomalies included decrease the body weight; increase the relative testis weight; and decrease the number of spermapogonia (type A and B), primary spermatocytes, spermatids and sperms in experimental groups I and II compared to control group. Our results showed the number of spermatozoa in experimental group I was 22.6±2.12, in experimental group II was 33.6±2.05 and in control group was 44.3±2.77 (p<0.05). Moreover in some experimental groups (I and II) shrinkage of seminiferous tubules and release of primary spermatocyte and spermatids were observed to the lumen of them.

Conclusion: It is concluded from the results of this work that treatment with methoxsalen with UVA can damage and disorganize seminiferous tubules and decrease spermatogenic cells.

No MeSH data available.


Related in: MedlinePlus