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Cecropins from Plutella xylostella and Their Interaction with Metarhizium anisopliae.

Ouyang L, Xu X, Freed S, Gao Y, Yu J, Wang S, Ju W, Zhang Y, Jin F - PLoS ONE (2015)

Bottom Line: Silencing of Spätzle and Dorsal separately caused the low expression of cecropins in the fat body, epidermis and hemocytes, and made the P.xylostella larvae more susceptible to M. anisopliae.Especially, Px-cecs showed higher activity against M. anisopliae than another selected fungi isolates.Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) revealed that cecropins exerted the vital morphological alterations to the spores of M. anisopliae.

View Article: PubMed Central - PubMed

Affiliation: College of Agriculture, South China Agricultural University, Guangzhou, 510642, P. R. China.

ABSTRACT
Cecropins are the most potent induced peptides to resist invading microorganisms. In the present study, two full length cDNA encoding cecropin2 (Px-cec2) and cecropin3 (Px-cec3) were obtained from P. xylostella by integrated analysis of genome and transcriptome data. qRT-PCR analysis revealed the high levels of transcripts of Px-cecs (Px-cec1, Px-cec2 and Px-cec3) in epidermis, fat body and hemocytes after 24, 30 and 36 h induction of Metarhizium anisopliae, respectively. Silencing of Spätzle and Dorsal separately caused the low expression of cecropins in the fat body, epidermis and hemocytes, and made the P.xylostella larvae more susceptible to M. anisopliae. Antimicrobial assays demonstrated that the purified recombinant cecropins, i.e., Px-cec1, Px-cec2 and Px-cec3, exerted a broad spectrum of antimicrobial activity against fungi, as well as Gram-positive and Gram-negative bacteria. Especially, Px-cecs showed higher activity against M. anisopliae than another selected fungi isolates. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) revealed that cecropins exerted the vital morphological alterations to the spores of M. anisopliae. Based on our results, cecropins played an imperative role in resisting infection of M. anisopliae, which will provide the foundation of biological control of insect pests by using cecorpins as a target in the future.

No MeSH data available.


Related in: MedlinePlus

Nucleotide sequence of Px-cec2 and Px-cec3 cDNA from P. xylostella and their deduced amino acids.The putative signal peptide is boxed, while the mature peptide is indicated in bold type, while the predicted transmembrane domain is shaded. The stop codon is marked as an asterisk.
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pone.0142451.g001: Nucleotide sequence of Px-cec2 and Px-cec3 cDNA from P. xylostella and their deduced amino acids.The putative signal peptide is boxed, while the mature peptide is indicated in bold type, while the predicted transmembrane domain is shaded. The stop codon is marked as an asterisk.

Mentions: Two full-length cDNA sequences of Px-cec2 and Px-cec3 were isolated from transcriptome and 5′ and 3′ RACE from of fungi infected P. xylostella in Fig 1. The nucleotide sequence of Px-cec2 (GenBank accession No. GU391356) consisted of 198 bp open reading frame (ORF) encoding a predicted polypeptide of 65 amino acids, while the deduced mature protein (Px-cec2) comprised of 39 amino acid residues having a predicted molecular weight of 4.1 kDa and a theoretical isoelectric point (pI) of 10.44. Whereas, the nucleotide sequence of Px-cec3 (GenBank accession No. KF960048) contained 186 bp ORF with 35 amino acids and a predicted molecular mass of 3.59 kDa and 10.57 pI (Fig 1).


Cecropins from Plutella xylostella and Their Interaction with Metarhizium anisopliae.

Ouyang L, Xu X, Freed S, Gao Y, Yu J, Wang S, Ju W, Zhang Y, Jin F - PLoS ONE (2015)

Nucleotide sequence of Px-cec2 and Px-cec3 cDNA from P. xylostella and their deduced amino acids.The putative signal peptide is boxed, while the mature peptide is indicated in bold type, while the predicted transmembrane domain is shaded. The stop codon is marked as an asterisk.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4636316&req=5

pone.0142451.g001: Nucleotide sequence of Px-cec2 and Px-cec3 cDNA from P. xylostella and their deduced amino acids.The putative signal peptide is boxed, while the mature peptide is indicated in bold type, while the predicted transmembrane domain is shaded. The stop codon is marked as an asterisk.
Mentions: Two full-length cDNA sequences of Px-cec2 and Px-cec3 were isolated from transcriptome and 5′ and 3′ RACE from of fungi infected P. xylostella in Fig 1. The nucleotide sequence of Px-cec2 (GenBank accession No. GU391356) consisted of 198 bp open reading frame (ORF) encoding a predicted polypeptide of 65 amino acids, while the deduced mature protein (Px-cec2) comprised of 39 amino acid residues having a predicted molecular weight of 4.1 kDa and a theoretical isoelectric point (pI) of 10.44. Whereas, the nucleotide sequence of Px-cec3 (GenBank accession No. KF960048) contained 186 bp ORF with 35 amino acids and a predicted molecular mass of 3.59 kDa and 10.57 pI (Fig 1).

Bottom Line: Silencing of Spätzle and Dorsal separately caused the low expression of cecropins in the fat body, epidermis and hemocytes, and made the P.xylostella larvae more susceptible to M. anisopliae.Especially, Px-cecs showed higher activity against M. anisopliae than another selected fungi isolates.Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) revealed that cecropins exerted the vital morphological alterations to the spores of M. anisopliae.

View Article: PubMed Central - PubMed

Affiliation: College of Agriculture, South China Agricultural University, Guangzhou, 510642, P. R. China.

ABSTRACT
Cecropins are the most potent induced peptides to resist invading microorganisms. In the present study, two full length cDNA encoding cecropin2 (Px-cec2) and cecropin3 (Px-cec3) were obtained from P. xylostella by integrated analysis of genome and transcriptome data. qRT-PCR analysis revealed the high levels of transcripts of Px-cecs (Px-cec1, Px-cec2 and Px-cec3) in epidermis, fat body and hemocytes after 24, 30 and 36 h induction of Metarhizium anisopliae, respectively. Silencing of Spätzle and Dorsal separately caused the low expression of cecropins in the fat body, epidermis and hemocytes, and made the P.xylostella larvae more susceptible to M. anisopliae. Antimicrobial assays demonstrated that the purified recombinant cecropins, i.e., Px-cec1, Px-cec2 and Px-cec3, exerted a broad spectrum of antimicrobial activity against fungi, as well as Gram-positive and Gram-negative bacteria. Especially, Px-cecs showed higher activity against M. anisopliae than another selected fungi isolates. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) revealed that cecropins exerted the vital morphological alterations to the spores of M. anisopliae. Based on our results, cecropins played an imperative role in resisting infection of M. anisopliae, which will provide the foundation of biological control of insect pests by using cecorpins as a target in the future.

No MeSH data available.


Related in: MedlinePlus