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Probiotics Blunt the Anti-Hypertensive Effect of Blueberry Feeding in Hypertensive Rats without Altering Hippuric Acid Production.

Blanton C, He Z, Gottschall-Pass KT, Sweeney MI - PLoS ONE (2015)

Bottom Line: Since probiotic bacteria produce bioactive metabolites from berry polyphenols that enhance the health benefits of berry consumption, we hypothesized that adding probiotics to a blueberry-enriched diet would augment the anti-hypertensive effects of blueberry consumption.Diet had a significant main effect on hippuric acid excretion (p<0.0001), with 2- and ~1.5-fold higher levels at weeks 4 and 8, respectively, in the BB and BB+PRO vs.Our findings show that adding probiotics to a blueberry-enriched diet does not enhance and actually may impair the anti-hypertensive effect of blueberry consumption.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition, Idaho State University, Pocatello, Idaho, United States of America.

ABSTRACT
Previously we showed that feeding polyphenol-rich wild blueberries to hypertensive rats lowered systolic blood pressure. Since probiotic bacteria produce bioactive metabolites from berry polyphenols that enhance the health benefits of berry consumption, we hypothesized that adding probiotics to a blueberry-enriched diet would augment the anti-hypertensive effects of blueberry consumption. Groups (n = 8) of male spontaneously hypertensive rats were fed one of four AIN '93G-based diets for 8 weeks: Control (CON); 3% freeze-dried wild blueberry (BB); 1% probiotic bacteria (PRO); or 3% BB + 1% PRO (BB+PRO). Blood pressure was measured at weeks 0, 2, 4, 6, and 8 by the tail-cuff method, and urine was collected at weeks 4 and 8 to determine markers of oxidative stress (F2-isoprostanes), nitric oxide synthesis (nitrites), and polyphenol metabolism (hippuric acid). Data were analyzed using mixed models ANOVA with repeated measures. Diet had a significant main effect on diastolic blood pressure (p = 0.046), with significantly lower measurements in the BB- vs. CON-fed rats (p = 0.035). Systolic blood pressure showed a similar but less pronounced response to diet (p = 0.220), again with the largest difference between the BB and CON groups. Absolute increase in blood pressure between weeks 0 and 8 tended to be smaller in the BB and PRO vs. CON and BB+PRO groups (systolic increase, p = 0.074; diastolic increase, p = 0.185). Diet had a significant main effect on hippuric acid excretion (p<0.0001), with 2- and ~1.5-fold higher levels at weeks 4 and 8, respectively, in the BB and BB+PRO vs. PRO and CON groups. Diet did not have a significant main effect on F2-isoprostane (p = 0.159) or nitrite excretion (p = 0.670). Our findings show that adding probiotics to a blueberry-enriched diet does not enhance and actually may impair the anti-hypertensive effect of blueberry consumption. However, probiotic bacteria are not interfering with blueberry polyphenol metabolism into hippuric acid.

No MeSH data available.


Related in: MedlinePlus

Systolic blood pressure.Systolic blood pressure of spontaneously hypertensive rats fed control (CON), 3% blueberry (BB), 1% probiotic (PRO), or 3% blueberry + 1% probiotic (BB+PRO) diet for 8 weeks. Data points are presented as means ± SEM for 8 rats in each diet group, with statistical analysis performed by mixed models ANOVA with repeated measures. Diet did not exert a significant main effect on systolic blood pressure across time points (p = 0.220) (S2 Dataset).
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pone.0142036.g002: Systolic blood pressure.Systolic blood pressure of spontaneously hypertensive rats fed control (CON), 3% blueberry (BB), 1% probiotic (PRO), or 3% blueberry + 1% probiotic (BB+PRO) diet for 8 weeks. Data points are presented as means ± SEM for 8 rats in each diet group, with statistical analysis performed by mixed models ANOVA with repeated measures. Diet did not exert a significant main effect on systolic blood pressure across time points (p = 0.220) (S2 Dataset).

Mentions: Diet had a significant main effect on diastolic BP across the experiment (p = 0.046), with significantly lower measurements in the BB-fed compared to CON-fed rats (post-hoc analysis with Tukey’s adjustment, p = 0.035, Fig 1). Systolic BP showed a similar but less pronounced response to diet (main effect of diet, p = 0.220), again with the largest difference occurring between the BB vs. CON groups (unadjusted p = 0.045; adjusted p = 0.187, Fig 2).


Probiotics Blunt the Anti-Hypertensive Effect of Blueberry Feeding in Hypertensive Rats without Altering Hippuric Acid Production.

Blanton C, He Z, Gottschall-Pass KT, Sweeney MI - PLoS ONE (2015)

Systolic blood pressure.Systolic blood pressure of spontaneously hypertensive rats fed control (CON), 3% blueberry (BB), 1% probiotic (PRO), or 3% blueberry + 1% probiotic (BB+PRO) diet for 8 weeks. Data points are presented as means ± SEM for 8 rats in each diet group, with statistical analysis performed by mixed models ANOVA with repeated measures. Diet did not exert a significant main effect on systolic blood pressure across time points (p = 0.220) (S2 Dataset).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4636313&req=5

pone.0142036.g002: Systolic blood pressure.Systolic blood pressure of spontaneously hypertensive rats fed control (CON), 3% blueberry (BB), 1% probiotic (PRO), or 3% blueberry + 1% probiotic (BB+PRO) diet for 8 weeks. Data points are presented as means ± SEM for 8 rats in each diet group, with statistical analysis performed by mixed models ANOVA with repeated measures. Diet did not exert a significant main effect on systolic blood pressure across time points (p = 0.220) (S2 Dataset).
Mentions: Diet had a significant main effect on diastolic BP across the experiment (p = 0.046), with significantly lower measurements in the BB-fed compared to CON-fed rats (post-hoc analysis with Tukey’s adjustment, p = 0.035, Fig 1). Systolic BP showed a similar but less pronounced response to diet (main effect of diet, p = 0.220), again with the largest difference occurring between the BB vs. CON groups (unadjusted p = 0.045; adjusted p = 0.187, Fig 2).

Bottom Line: Since probiotic bacteria produce bioactive metabolites from berry polyphenols that enhance the health benefits of berry consumption, we hypothesized that adding probiotics to a blueberry-enriched diet would augment the anti-hypertensive effects of blueberry consumption.Diet had a significant main effect on hippuric acid excretion (p<0.0001), with 2- and ~1.5-fold higher levels at weeks 4 and 8, respectively, in the BB and BB+PRO vs.Our findings show that adding probiotics to a blueberry-enriched diet does not enhance and actually may impair the anti-hypertensive effect of blueberry consumption.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition, Idaho State University, Pocatello, Idaho, United States of America.

ABSTRACT
Previously we showed that feeding polyphenol-rich wild blueberries to hypertensive rats lowered systolic blood pressure. Since probiotic bacteria produce bioactive metabolites from berry polyphenols that enhance the health benefits of berry consumption, we hypothesized that adding probiotics to a blueberry-enriched diet would augment the anti-hypertensive effects of blueberry consumption. Groups (n = 8) of male spontaneously hypertensive rats were fed one of four AIN '93G-based diets for 8 weeks: Control (CON); 3% freeze-dried wild blueberry (BB); 1% probiotic bacteria (PRO); or 3% BB + 1% PRO (BB+PRO). Blood pressure was measured at weeks 0, 2, 4, 6, and 8 by the tail-cuff method, and urine was collected at weeks 4 and 8 to determine markers of oxidative stress (F2-isoprostanes), nitric oxide synthesis (nitrites), and polyphenol metabolism (hippuric acid). Data were analyzed using mixed models ANOVA with repeated measures. Diet had a significant main effect on diastolic blood pressure (p = 0.046), with significantly lower measurements in the BB- vs. CON-fed rats (p = 0.035). Systolic blood pressure showed a similar but less pronounced response to diet (p = 0.220), again with the largest difference between the BB and CON groups. Absolute increase in blood pressure between weeks 0 and 8 tended to be smaller in the BB and PRO vs. CON and BB+PRO groups (systolic increase, p = 0.074; diastolic increase, p = 0.185). Diet had a significant main effect on hippuric acid excretion (p<0.0001), with 2- and ~1.5-fold higher levels at weeks 4 and 8, respectively, in the BB and BB+PRO vs. PRO and CON groups. Diet did not have a significant main effect on F2-isoprostane (p = 0.159) or nitrite excretion (p = 0.670). Our findings show that adding probiotics to a blueberry-enriched diet does not enhance and actually may impair the anti-hypertensive effect of blueberry consumption. However, probiotic bacteria are not interfering with blueberry polyphenol metabolism into hippuric acid.

No MeSH data available.


Related in: MedlinePlus