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Pretreatment with Resveratrol Prevents Neuronal Injury and Cognitive Deficits Induced by Perinatal Hypoxia-Ischemia in Rats.

Arteaga O, Revuelta M, Urigüen L, Álvarez A, Montalvo H, Hilario E - PLoS ONE (2015)

Bottom Line: Our results indicate that pretreatment with resveratrol protects against brain damage, reducing infarct volume, preserving myelination and minimizing the astroglial reactive response.Moreover its neuroprotective effect was found to be long lasting, as behavioral outcomes were significantly improved at adulthood.We speculate that one of the mechanisms for this neuroprotection may be related to the maintenance of the mitochondrial inner membrane integrity and potential, and to the reduction of reactive oxygen species.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology & Histology, School of Medicine & Dentistry, University of the Basque Country (UPV/EHU), Leioa, Bizkaia, Spain.

ABSTRACT
Despite advances in neonatal care, hypoxic-ischemic brain injury is still a serious clinical problem, which is responsible for many cases of perinatal mortality, cerebral palsy, motor impairment and cognitive deficits. Resveratrol, a natural polyphenol with important anti-oxidant and anti-inflammatory properties, is present in grapevines, peanuts and pomegranates. The aim of the present work was to evaluate the possible neuroprotective effect of resveratrol when administered before or immediately after a hypoxic-ischemic brain event in neonatal rats by analyzing brain damage, the mitochondrial status and long-term cognitive impairment. Our results indicate that pretreatment with resveratrol protects against brain damage, reducing infarct volume, preserving myelination and minimizing the astroglial reactive response. Moreover its neuroprotective effect was found to be long lasting, as behavioral outcomes were significantly improved at adulthood. We speculate that one of the mechanisms for this neuroprotection may be related to the maintenance of the mitochondrial inner membrane integrity and potential, and to the reduction of reactive oxygen species. Curiously, none of these protective features was observed when resveratrol was administered immediately after hypoxia-ischemia.

No MeSH data available.


Related in: MedlinePlus

Effect of neonatal hypoxia-ischemia and pretreatment with resveratrol on choice accuracy in the discrete-trial delayed spatial alternation task (T-maze) in adult animals on P90.(A) Control (n = 16) and HI rats (n = 14), as well as RVT pretreated animals (n = 10) made a similar number of correct choices in the T-maze at 10 s delay. (B) In contrast, HI animals made significantly fewer correct choices after the 40 s delay. Impaired memory performance (percentage of correct trials) due to hypoxia-ischemia was reverted by RVT pre-administration. Asterisks denote the significance levels when compared to the control group (***P<0.0001). The hash symbols denote the significance levels when compared to the HI group (###P<0.0001).
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pone.0142424.g013: Effect of neonatal hypoxia-ischemia and pretreatment with resveratrol on choice accuracy in the discrete-trial delayed spatial alternation task (T-maze) in adult animals on P90.(A) Control (n = 16) and HI rats (n = 14), as well as RVT pretreated animals (n = 10) made a similar number of correct choices in the T-maze at 10 s delay. (B) In contrast, HI animals made significantly fewer correct choices after the 40 s delay. Impaired memory performance (percentage of correct trials) due to hypoxia-ischemia was reverted by RVT pre-administration. Asterisks denote the significance levels when compared to the control group (***P<0.0001). The hash symbols denote the significance levels when compared to the HI group (###P<0.0001).

Mentions: At a 10 s delay interval (Fig 13A), no differences were found between groups. In contrast, at a 40 s delay interval ([F(2,37) = 32.57, P < 0.0001]), HI animals made significantly fewer correct choices (Fig 13B) when compared to control animals. RVT reversed these changes and significantly increased the number of correct choices when compared to HI rats. The results indicate a working memory dysfunction induced by hypoxia-ischemia which is prevented by the acute administration of RVT before the ischemic event.


Pretreatment with Resveratrol Prevents Neuronal Injury and Cognitive Deficits Induced by Perinatal Hypoxia-Ischemia in Rats.

Arteaga O, Revuelta M, Urigüen L, Álvarez A, Montalvo H, Hilario E - PLoS ONE (2015)

Effect of neonatal hypoxia-ischemia and pretreatment with resveratrol on choice accuracy in the discrete-trial delayed spatial alternation task (T-maze) in adult animals on P90.(A) Control (n = 16) and HI rats (n = 14), as well as RVT pretreated animals (n = 10) made a similar number of correct choices in the T-maze at 10 s delay. (B) In contrast, HI animals made significantly fewer correct choices after the 40 s delay. Impaired memory performance (percentage of correct trials) due to hypoxia-ischemia was reverted by RVT pre-administration. Asterisks denote the significance levels when compared to the control group (***P<0.0001). The hash symbols denote the significance levels when compared to the HI group (###P<0.0001).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4636303&req=5

pone.0142424.g013: Effect of neonatal hypoxia-ischemia and pretreatment with resveratrol on choice accuracy in the discrete-trial delayed spatial alternation task (T-maze) in adult animals on P90.(A) Control (n = 16) and HI rats (n = 14), as well as RVT pretreated animals (n = 10) made a similar number of correct choices in the T-maze at 10 s delay. (B) In contrast, HI animals made significantly fewer correct choices after the 40 s delay. Impaired memory performance (percentage of correct trials) due to hypoxia-ischemia was reverted by RVT pre-administration. Asterisks denote the significance levels when compared to the control group (***P<0.0001). The hash symbols denote the significance levels when compared to the HI group (###P<0.0001).
Mentions: At a 10 s delay interval (Fig 13A), no differences were found between groups. In contrast, at a 40 s delay interval ([F(2,37) = 32.57, P < 0.0001]), HI animals made significantly fewer correct choices (Fig 13B) when compared to control animals. RVT reversed these changes and significantly increased the number of correct choices when compared to HI rats. The results indicate a working memory dysfunction induced by hypoxia-ischemia which is prevented by the acute administration of RVT before the ischemic event.

Bottom Line: Our results indicate that pretreatment with resveratrol protects against brain damage, reducing infarct volume, preserving myelination and minimizing the astroglial reactive response.Moreover its neuroprotective effect was found to be long lasting, as behavioral outcomes were significantly improved at adulthood.We speculate that one of the mechanisms for this neuroprotection may be related to the maintenance of the mitochondrial inner membrane integrity and potential, and to the reduction of reactive oxygen species.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology & Histology, School of Medicine & Dentistry, University of the Basque Country (UPV/EHU), Leioa, Bizkaia, Spain.

ABSTRACT
Despite advances in neonatal care, hypoxic-ischemic brain injury is still a serious clinical problem, which is responsible for many cases of perinatal mortality, cerebral palsy, motor impairment and cognitive deficits. Resveratrol, a natural polyphenol with important anti-oxidant and anti-inflammatory properties, is present in grapevines, peanuts and pomegranates. The aim of the present work was to evaluate the possible neuroprotective effect of resveratrol when administered before or immediately after a hypoxic-ischemic brain event in neonatal rats by analyzing brain damage, the mitochondrial status and long-term cognitive impairment. Our results indicate that pretreatment with resveratrol protects against brain damage, reducing infarct volume, preserving myelination and minimizing the astroglial reactive response. Moreover its neuroprotective effect was found to be long lasting, as behavioral outcomes were significantly improved at adulthood. We speculate that one of the mechanisms for this neuroprotection may be related to the maintenance of the mitochondrial inner membrane integrity and potential, and to the reduction of reactive oxygen species. Curiously, none of these protective features was observed when resveratrol was administered immediately after hypoxia-ischemia.

No MeSH data available.


Related in: MedlinePlus