Limits...
Pretreatment with Resveratrol Prevents Neuronal Injury and Cognitive Deficits Induced by Perinatal Hypoxia-Ischemia in Rats.

Arteaga O, Revuelta M, Urigüen L, Álvarez A, Montalvo H, Hilario E - PLoS ONE (2015)

Bottom Line: Our results indicate that pretreatment with resveratrol protects against brain damage, reducing infarct volume, preserving myelination and minimizing the astroglial reactive response.Moreover its neuroprotective effect was found to be long lasting, as behavioral outcomes were significantly improved at adulthood.We speculate that one of the mechanisms for this neuroprotection may be related to the maintenance of the mitochondrial inner membrane integrity and potential, and to the reduction of reactive oxygen species.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology & Histology, School of Medicine & Dentistry, University of the Basque Country (UPV/EHU), Leioa, Bizkaia, Spain.

ABSTRACT
Despite advances in neonatal care, hypoxic-ischemic brain injury is still a serious clinical problem, which is responsible for many cases of perinatal mortality, cerebral palsy, motor impairment and cognitive deficits. Resveratrol, a natural polyphenol with important anti-oxidant and anti-inflammatory properties, is present in grapevines, peanuts and pomegranates. The aim of the present work was to evaluate the possible neuroprotective effect of resveratrol when administered before or immediately after a hypoxic-ischemic brain event in neonatal rats by analyzing brain damage, the mitochondrial status and long-term cognitive impairment. Our results indicate that pretreatment with resveratrol protects against brain damage, reducing infarct volume, preserving myelination and minimizing the astroglial reactive response. Moreover its neuroprotective effect was found to be long lasting, as behavioral outcomes were significantly improved at adulthood. We speculate that one of the mechanisms for this neuroprotection may be related to the maintenance of the mitochondrial inner membrane integrity and potential, and to the reduction of reactive oxygen species. Curiously, none of these protective features was observed when resveratrol was administered immediately after hypoxia-ischemia.

No MeSH data available.


Related in: MedlinePlus

Effect of brain hypoxia-ischemia on the production of reactive oxygen species in suspension of acutely isolated cells, measured using DCFH-DA.Percentage of DCFH-DA positive cells at different time points after hypoxia-ischemia: (A) 0 h, (B) 3 h and (C) 12 h. Relative fluorescence intensity of cells with in vivo marker DCFH-DA at different time points after hypoxia-ischemia; (D) 0 h, (E) 3 h and (F) 12 h, in control (n≥5), HI (n≥5) and animals pretreated with resveratrol (n≥5) groups. Asterisks denote the significance levels when compared to the control group (***P<0.0001 *P<0.05). The hash symbols denote the significance levels when compared to the hypoxia-ischemia group (###P<0.0001 or #P<0.05).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4636303&req=5

pone.0142424.g010: Effect of brain hypoxia-ischemia on the production of reactive oxygen species in suspension of acutely isolated cells, measured using DCFH-DA.Percentage of DCFH-DA positive cells at different time points after hypoxia-ischemia: (A) 0 h, (B) 3 h and (C) 12 h. Relative fluorescence intensity of cells with in vivo marker DCFH-DA at different time points after hypoxia-ischemia; (D) 0 h, (E) 3 h and (F) 12 h, in control (n≥5), HI (n≥5) and animals pretreated with resveratrol (n≥5) groups. Asterisks denote the significance levels when compared to the control group (***P<0.0001 *P<0.05). The hash symbols denote the significance levels when compared to the hypoxia-ischemia group (###P<0.0001 or #P<0.05).

Mentions: Oxygen reactive species were detected using the DCFH-DA fluorochrome. At 0 h ([F(2,18) = 216,9, P < 0.0001]) while the HI group showed a decrease in the percentage of DCFH positive cells, the treated group presented similar percentage values which were statistically similar to those of the control group (Fig 10A). At 3 h and 12 h, no statistically significant differences were found among all the groups (Fig 10B and 10C).


Pretreatment with Resveratrol Prevents Neuronal Injury and Cognitive Deficits Induced by Perinatal Hypoxia-Ischemia in Rats.

Arteaga O, Revuelta M, Urigüen L, Álvarez A, Montalvo H, Hilario E - PLoS ONE (2015)

Effect of brain hypoxia-ischemia on the production of reactive oxygen species in suspension of acutely isolated cells, measured using DCFH-DA.Percentage of DCFH-DA positive cells at different time points after hypoxia-ischemia: (A) 0 h, (B) 3 h and (C) 12 h. Relative fluorescence intensity of cells with in vivo marker DCFH-DA at different time points after hypoxia-ischemia; (D) 0 h, (E) 3 h and (F) 12 h, in control (n≥5), HI (n≥5) and animals pretreated with resveratrol (n≥5) groups. Asterisks denote the significance levels when compared to the control group (***P<0.0001 *P<0.05). The hash symbols denote the significance levels when compared to the hypoxia-ischemia group (###P<0.0001 or #P<0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4636303&req=5

pone.0142424.g010: Effect of brain hypoxia-ischemia on the production of reactive oxygen species in suspension of acutely isolated cells, measured using DCFH-DA.Percentage of DCFH-DA positive cells at different time points after hypoxia-ischemia: (A) 0 h, (B) 3 h and (C) 12 h. Relative fluorescence intensity of cells with in vivo marker DCFH-DA at different time points after hypoxia-ischemia; (D) 0 h, (E) 3 h and (F) 12 h, in control (n≥5), HI (n≥5) and animals pretreated with resveratrol (n≥5) groups. Asterisks denote the significance levels when compared to the control group (***P<0.0001 *P<0.05). The hash symbols denote the significance levels when compared to the hypoxia-ischemia group (###P<0.0001 or #P<0.05).
Mentions: Oxygen reactive species were detected using the DCFH-DA fluorochrome. At 0 h ([F(2,18) = 216,9, P < 0.0001]) while the HI group showed a decrease in the percentage of DCFH positive cells, the treated group presented similar percentage values which were statistically similar to those of the control group (Fig 10A). At 3 h and 12 h, no statistically significant differences were found among all the groups (Fig 10B and 10C).

Bottom Line: Our results indicate that pretreatment with resveratrol protects against brain damage, reducing infarct volume, preserving myelination and minimizing the astroglial reactive response.Moreover its neuroprotective effect was found to be long lasting, as behavioral outcomes were significantly improved at adulthood.We speculate that one of the mechanisms for this neuroprotection may be related to the maintenance of the mitochondrial inner membrane integrity and potential, and to the reduction of reactive oxygen species.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology & Histology, School of Medicine & Dentistry, University of the Basque Country (UPV/EHU), Leioa, Bizkaia, Spain.

ABSTRACT
Despite advances in neonatal care, hypoxic-ischemic brain injury is still a serious clinical problem, which is responsible for many cases of perinatal mortality, cerebral palsy, motor impairment and cognitive deficits. Resveratrol, a natural polyphenol with important anti-oxidant and anti-inflammatory properties, is present in grapevines, peanuts and pomegranates. The aim of the present work was to evaluate the possible neuroprotective effect of resveratrol when administered before or immediately after a hypoxic-ischemic brain event in neonatal rats by analyzing brain damage, the mitochondrial status and long-term cognitive impairment. Our results indicate that pretreatment with resveratrol protects against brain damage, reducing infarct volume, preserving myelination and minimizing the astroglial reactive response. Moreover its neuroprotective effect was found to be long lasting, as behavioral outcomes were significantly improved at adulthood. We speculate that one of the mechanisms for this neuroprotection may be related to the maintenance of the mitochondrial inner membrane integrity and potential, and to the reduction of reactive oxygen species. Curiously, none of these protective features was observed when resveratrol was administered immediately after hypoxia-ischemia.

No MeSH data available.


Related in: MedlinePlus