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Pretreatment with Resveratrol Prevents Neuronal Injury and Cognitive Deficits Induced by Perinatal Hypoxia-Ischemia in Rats.

Arteaga O, Revuelta M, Urigüen L, Álvarez A, Montalvo H, Hilario E - PLoS ONE (2015)

Bottom Line: Our results indicate that pretreatment with resveratrol protects against brain damage, reducing infarct volume, preserving myelination and minimizing the astroglial reactive response.Moreover its neuroprotective effect was found to be long lasting, as behavioral outcomes were significantly improved at adulthood.We speculate that one of the mechanisms for this neuroprotection may be related to the maintenance of the mitochondrial inner membrane integrity and potential, and to the reduction of reactive oxygen species.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology & Histology, School of Medicine & Dentistry, University of the Basque Country (UPV/EHU), Leioa, Bizkaia, Spain.

ABSTRACT
Despite advances in neonatal care, hypoxic-ischemic brain injury is still a serious clinical problem, which is responsible for many cases of perinatal mortality, cerebral palsy, motor impairment and cognitive deficits. Resveratrol, a natural polyphenol with important anti-oxidant and anti-inflammatory properties, is present in grapevines, peanuts and pomegranates. The aim of the present work was to evaluate the possible neuroprotective effect of resveratrol when administered before or immediately after a hypoxic-ischemic brain event in neonatal rats by analyzing brain damage, the mitochondrial status and long-term cognitive impairment. Our results indicate that pretreatment with resveratrol protects against brain damage, reducing infarct volume, preserving myelination and minimizing the astroglial reactive response. Moreover its neuroprotective effect was found to be long lasting, as behavioral outcomes were significantly improved at adulthood. We speculate that one of the mechanisms for this neuroprotection may be related to the maintenance of the mitochondrial inner membrane integrity and potential, and to the reduction of reactive oxygen species. Curiously, none of these protective features was observed when resveratrol was administered immediately after hypoxia-ischemia.

No MeSH data available.


Related in: MedlinePlus

Representative light microphotographs of myelin basic protein (MBP)-stained brain sections and comparison of loss of MBP immunostaining in the external capsule of different groups: 14-day-old control (n = 5) (A), HI (n = 14) (B), RVT-b (n = 14) (C) and RVT-a (n = 7) (D) (scale bar: 40 μm).In the histogram (E), the extent of tissue injury, expressed as a ratio of left-to-right hemispheric MBP immunostaining is represented. Asterisks denote the significance levels when compared to the control group (**P<0.0001). Hashes denote the significance level when compared to the HI group (###P<0.0001).
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pone.0142424.g005: Representative light microphotographs of myelin basic protein (MBP)-stained brain sections and comparison of loss of MBP immunostaining in the external capsule of different groups: 14-day-old control (n = 5) (A), HI (n = 14) (B), RVT-b (n = 14) (C) and RVT-a (n = 7) (D) (scale bar: 40 μm).In the histogram (E), the extent of tissue injury, expressed as a ratio of left-to-right hemispheric MBP immunostaining is represented. Asterisks denote the significance levels when compared to the control group (**P<0.0001). Hashes denote the significance level when compared to the HI group (###P<0.0001).

Mentions: A substantial loss of ipsilateral MBP immunostaining was observed in both external capsule and striatum in P14 animals that had been subjected one week earlier to hypoxia-ischemia with respect to controls (Figs 5 and 6). This loss was absent with resveratrol pretreatment, but not when resveratrol was administered after the injury.


Pretreatment with Resveratrol Prevents Neuronal Injury and Cognitive Deficits Induced by Perinatal Hypoxia-Ischemia in Rats.

Arteaga O, Revuelta M, Urigüen L, Álvarez A, Montalvo H, Hilario E - PLoS ONE (2015)

Representative light microphotographs of myelin basic protein (MBP)-stained brain sections and comparison of loss of MBP immunostaining in the external capsule of different groups: 14-day-old control (n = 5) (A), HI (n = 14) (B), RVT-b (n = 14) (C) and RVT-a (n = 7) (D) (scale bar: 40 μm).In the histogram (E), the extent of tissue injury, expressed as a ratio of left-to-right hemispheric MBP immunostaining is represented. Asterisks denote the significance levels when compared to the control group (**P<0.0001). Hashes denote the significance level when compared to the HI group (###P<0.0001).
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4636303&req=5

pone.0142424.g005: Representative light microphotographs of myelin basic protein (MBP)-stained brain sections and comparison of loss of MBP immunostaining in the external capsule of different groups: 14-day-old control (n = 5) (A), HI (n = 14) (B), RVT-b (n = 14) (C) and RVT-a (n = 7) (D) (scale bar: 40 μm).In the histogram (E), the extent of tissue injury, expressed as a ratio of left-to-right hemispheric MBP immunostaining is represented. Asterisks denote the significance levels when compared to the control group (**P<0.0001). Hashes denote the significance level when compared to the HI group (###P<0.0001).
Mentions: A substantial loss of ipsilateral MBP immunostaining was observed in both external capsule and striatum in P14 animals that had been subjected one week earlier to hypoxia-ischemia with respect to controls (Figs 5 and 6). This loss was absent with resveratrol pretreatment, but not when resveratrol was administered after the injury.

Bottom Line: Our results indicate that pretreatment with resveratrol protects against brain damage, reducing infarct volume, preserving myelination and minimizing the astroglial reactive response.Moreover its neuroprotective effect was found to be long lasting, as behavioral outcomes were significantly improved at adulthood.We speculate that one of the mechanisms for this neuroprotection may be related to the maintenance of the mitochondrial inner membrane integrity and potential, and to the reduction of reactive oxygen species.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology & Histology, School of Medicine & Dentistry, University of the Basque Country (UPV/EHU), Leioa, Bizkaia, Spain.

ABSTRACT
Despite advances in neonatal care, hypoxic-ischemic brain injury is still a serious clinical problem, which is responsible for many cases of perinatal mortality, cerebral palsy, motor impairment and cognitive deficits. Resveratrol, a natural polyphenol with important anti-oxidant and anti-inflammatory properties, is present in grapevines, peanuts and pomegranates. The aim of the present work was to evaluate the possible neuroprotective effect of resveratrol when administered before or immediately after a hypoxic-ischemic brain event in neonatal rats by analyzing brain damage, the mitochondrial status and long-term cognitive impairment. Our results indicate that pretreatment with resveratrol protects against brain damage, reducing infarct volume, preserving myelination and minimizing the astroglial reactive response. Moreover its neuroprotective effect was found to be long lasting, as behavioral outcomes were significantly improved at adulthood. We speculate that one of the mechanisms for this neuroprotection may be related to the maintenance of the mitochondrial inner membrane integrity and potential, and to the reduction of reactive oxygen species. Curiously, none of these protective features was observed when resveratrol was administered immediately after hypoxia-ischemia.

No MeSH data available.


Related in: MedlinePlus