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Pretreatment with Resveratrol Prevents Neuronal Injury and Cognitive Deficits Induced by Perinatal Hypoxia-Ischemia in Rats.

Arteaga O, Revuelta M, Urigüen L, Álvarez A, Montalvo H, Hilario E - PLoS ONE (2015)

Bottom Line: Our results indicate that pretreatment with resveratrol protects against brain damage, reducing infarct volume, preserving myelination and minimizing the astroglial reactive response.Moreover its neuroprotective effect was found to be long lasting, as behavioral outcomes were significantly improved at adulthood.We speculate that one of the mechanisms for this neuroprotection may be related to the maintenance of the mitochondrial inner membrane integrity and potential, and to the reduction of reactive oxygen species.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology & Histology, School of Medicine & Dentistry, University of the Basque Country (UPV/EHU), Leioa, Bizkaia, Spain.

ABSTRACT
Despite advances in neonatal care, hypoxic-ischemic brain injury is still a serious clinical problem, which is responsible for many cases of perinatal mortality, cerebral palsy, motor impairment and cognitive deficits. Resveratrol, a natural polyphenol with important anti-oxidant and anti-inflammatory properties, is present in grapevines, peanuts and pomegranates. The aim of the present work was to evaluate the possible neuroprotective effect of resveratrol when administered before or immediately after a hypoxic-ischemic brain event in neonatal rats by analyzing brain damage, the mitochondrial status and long-term cognitive impairment. Our results indicate that pretreatment with resveratrol protects against brain damage, reducing infarct volume, preserving myelination and minimizing the astroglial reactive response. Moreover its neuroprotective effect was found to be long lasting, as behavioral outcomes were significantly improved at adulthood. We speculate that one of the mechanisms for this neuroprotection may be related to the maintenance of the mitochondrial inner membrane integrity and potential, and to the reduction of reactive oxygen species. Curiously, none of these protective features was observed when resveratrol was administered immediately after hypoxia-ischemia.

No MeSH data available.


Related in: MedlinePlus

Representative confocal microphotographs of glial fibrillary acidic protein (GFAP)-immunoreactivity in brain sections counterstained with DAPI.On postnatal day 14, GFAP immunoreactivity (green) was particularly pronounced in the vicinity of damaged areas and this reactivity was substantially reduced in animals pre-treated with resveratrol. Scale bar: 40 μm.
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pone.0142424.g004: Representative confocal microphotographs of glial fibrillary acidic protein (GFAP)-immunoreactivity in brain sections counterstained with DAPI.On postnatal day 14, GFAP immunoreactivity (green) was particularly pronounced in the vicinity of damaged areas and this reactivity was substantially reduced in animals pre-treated with resveratrol. Scale bar: 40 μm.

Mentions: GFAP astrogliosis was found in animals with hypoxic-ischemic injury, particularly in regions near dead or dying cells, such as the CA1 and dentate gyrus areas of the hippocampus, while control cases showed low levels of GFAP immunoreactivity (Fig 4). This reactive astrocyte response was diminished when resveratrol was administered before hypoxia, but not after it, demonstrating that pretreatment with resveratrol reduced the astroglial reactive response.


Pretreatment with Resveratrol Prevents Neuronal Injury and Cognitive Deficits Induced by Perinatal Hypoxia-Ischemia in Rats.

Arteaga O, Revuelta M, Urigüen L, Álvarez A, Montalvo H, Hilario E - PLoS ONE (2015)

Representative confocal microphotographs of glial fibrillary acidic protein (GFAP)-immunoreactivity in brain sections counterstained with DAPI.On postnatal day 14, GFAP immunoreactivity (green) was particularly pronounced in the vicinity of damaged areas and this reactivity was substantially reduced in animals pre-treated with resveratrol. Scale bar: 40 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4636303&req=5

pone.0142424.g004: Representative confocal microphotographs of glial fibrillary acidic protein (GFAP)-immunoreactivity in brain sections counterstained with DAPI.On postnatal day 14, GFAP immunoreactivity (green) was particularly pronounced in the vicinity of damaged areas and this reactivity was substantially reduced in animals pre-treated with resveratrol. Scale bar: 40 μm.
Mentions: GFAP astrogliosis was found in animals with hypoxic-ischemic injury, particularly in regions near dead or dying cells, such as the CA1 and dentate gyrus areas of the hippocampus, while control cases showed low levels of GFAP immunoreactivity (Fig 4). This reactive astrocyte response was diminished when resveratrol was administered before hypoxia, but not after it, demonstrating that pretreatment with resveratrol reduced the astroglial reactive response.

Bottom Line: Our results indicate that pretreatment with resveratrol protects against brain damage, reducing infarct volume, preserving myelination and minimizing the astroglial reactive response.Moreover its neuroprotective effect was found to be long lasting, as behavioral outcomes were significantly improved at adulthood.We speculate that one of the mechanisms for this neuroprotection may be related to the maintenance of the mitochondrial inner membrane integrity and potential, and to the reduction of reactive oxygen species.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology & Histology, School of Medicine & Dentistry, University of the Basque Country (UPV/EHU), Leioa, Bizkaia, Spain.

ABSTRACT
Despite advances in neonatal care, hypoxic-ischemic brain injury is still a serious clinical problem, which is responsible for many cases of perinatal mortality, cerebral palsy, motor impairment and cognitive deficits. Resveratrol, a natural polyphenol with important anti-oxidant and anti-inflammatory properties, is present in grapevines, peanuts and pomegranates. The aim of the present work was to evaluate the possible neuroprotective effect of resveratrol when administered before or immediately after a hypoxic-ischemic brain event in neonatal rats by analyzing brain damage, the mitochondrial status and long-term cognitive impairment. Our results indicate that pretreatment with resveratrol protects against brain damage, reducing infarct volume, preserving myelination and minimizing the astroglial reactive response. Moreover its neuroprotective effect was found to be long lasting, as behavioral outcomes were significantly improved at adulthood. We speculate that one of the mechanisms for this neuroprotection may be related to the maintenance of the mitochondrial inner membrane integrity and potential, and to the reduction of reactive oxygen species. Curiously, none of these protective features was observed when resveratrol was administered immediately after hypoxia-ischemia.

No MeSH data available.


Related in: MedlinePlus