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Pretreatment with Resveratrol Prevents Neuronal Injury and Cognitive Deficits Induced by Perinatal Hypoxia-Ischemia in Rats.

Arteaga O, Revuelta M, Urigüen L, Álvarez A, Montalvo H, Hilario E - PLoS ONE (2015)

Bottom Line: Our results indicate that pretreatment with resveratrol protects against brain damage, reducing infarct volume, preserving myelination and minimizing the astroglial reactive response.Moreover its neuroprotective effect was found to be long lasting, as behavioral outcomes were significantly improved at adulthood.We speculate that one of the mechanisms for this neuroprotection may be related to the maintenance of the mitochondrial inner membrane integrity and potential, and to the reduction of reactive oxygen species.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology & Histology, School of Medicine & Dentistry, University of the Basque Country (UPV/EHU), Leioa, Bizkaia, Spain.

ABSTRACT
Despite advances in neonatal care, hypoxic-ischemic brain injury is still a serious clinical problem, which is responsible for many cases of perinatal mortality, cerebral palsy, motor impairment and cognitive deficits. Resveratrol, a natural polyphenol with important anti-oxidant and anti-inflammatory properties, is present in grapevines, peanuts and pomegranates. The aim of the present work was to evaluate the possible neuroprotective effect of resveratrol when administered before or immediately after a hypoxic-ischemic brain event in neonatal rats by analyzing brain damage, the mitochondrial status and long-term cognitive impairment. Our results indicate that pretreatment with resveratrol protects against brain damage, reducing infarct volume, preserving myelination and minimizing the astroglial reactive response. Moreover its neuroprotective effect was found to be long lasting, as behavioral outcomes were significantly improved at adulthood. We speculate that one of the mechanisms for this neuroprotection may be related to the maintenance of the mitochondrial inner membrane integrity and potential, and to the reduction of reactive oxygen species. Curiously, none of these protective features was observed when resveratrol was administered immediately after hypoxia-ischemia.

No MeSH data available.


Related in: MedlinePlus

Representative microphotographs of Nissl-stained brain sections in animals exposed to hypoxia-ischemia at P7 and evaluated at P14.Individual fields represent different experimental groups and different areas of the hippocampus (CA 1, CA 2–3 and DG) and from the parietal cortex (CTX), with high magnification insets in CTX. Cell loss was especially evident in the DG and parietal cortex (arrows) in the hypoxia-ischemia and resveratrol post-treatment groups. In contrast, the resveratrol pre-treated group showed a remarkable conservation in the cellularity of the different studied areas with respect to the HI group. Scale bar: 50 μm.
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pone.0142424.g002: Representative microphotographs of Nissl-stained brain sections in animals exposed to hypoxia-ischemia at P7 and evaluated at P14.Individual fields represent different experimental groups and different areas of the hippocampus (CA 1, CA 2–3 and DG) and from the parietal cortex (CTX), with high magnification insets in CTX. Cell loss was especially evident in the DG and parietal cortex (arrows) in the hypoxia-ischemia and resveratrol post-treatment groups. In contrast, the resveratrol pre-treated group showed a remarkable conservation in the cellularity of the different studied areas with respect to the HI group. Scale bar: 50 μm.

Mentions: The hippocampus and the parietal cortex of hypoxic-ischemic animals displayed significant evidence of infarction, whereas those of control animals did not. In Nissl stained brain sections, hypoxia-ischemia induced a significant cell loss (see arrows in Fig 2). Moreover, asphyctic animals showed swollen and deformed neurons especially in the ipsilateral CA 1 and CTX areas. In contrast, only mild cell loss and a few damaged neurons were observed in slices in all brain regions studied from animals pretreated with resveratrol, Overall, the subfields in the hippocampus and the parietal cortex were similar in structure to those of the control group. Animals treated with resveratrol immediately after hypoxia presented extensive cell loss, as well as swollen and deformed cells, with overall characteristics which were similar to those exhibited by the hypoxia-ischemia group.


Pretreatment with Resveratrol Prevents Neuronal Injury and Cognitive Deficits Induced by Perinatal Hypoxia-Ischemia in Rats.

Arteaga O, Revuelta M, Urigüen L, Álvarez A, Montalvo H, Hilario E - PLoS ONE (2015)

Representative microphotographs of Nissl-stained brain sections in animals exposed to hypoxia-ischemia at P7 and evaluated at P14.Individual fields represent different experimental groups and different areas of the hippocampus (CA 1, CA 2–3 and DG) and from the parietal cortex (CTX), with high magnification insets in CTX. Cell loss was especially evident in the DG and parietal cortex (arrows) in the hypoxia-ischemia and resveratrol post-treatment groups. In contrast, the resveratrol pre-treated group showed a remarkable conservation in the cellularity of the different studied areas with respect to the HI group. Scale bar: 50 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4636303&req=5

pone.0142424.g002: Representative microphotographs of Nissl-stained brain sections in animals exposed to hypoxia-ischemia at P7 and evaluated at P14.Individual fields represent different experimental groups and different areas of the hippocampus (CA 1, CA 2–3 and DG) and from the parietal cortex (CTX), with high magnification insets in CTX. Cell loss was especially evident in the DG and parietal cortex (arrows) in the hypoxia-ischemia and resveratrol post-treatment groups. In contrast, the resveratrol pre-treated group showed a remarkable conservation in the cellularity of the different studied areas with respect to the HI group. Scale bar: 50 μm.
Mentions: The hippocampus and the parietal cortex of hypoxic-ischemic animals displayed significant evidence of infarction, whereas those of control animals did not. In Nissl stained brain sections, hypoxia-ischemia induced a significant cell loss (see arrows in Fig 2). Moreover, asphyctic animals showed swollen and deformed neurons especially in the ipsilateral CA 1 and CTX areas. In contrast, only mild cell loss and a few damaged neurons were observed in slices in all brain regions studied from animals pretreated with resveratrol, Overall, the subfields in the hippocampus and the parietal cortex were similar in structure to those of the control group. Animals treated with resveratrol immediately after hypoxia presented extensive cell loss, as well as swollen and deformed cells, with overall characteristics which were similar to those exhibited by the hypoxia-ischemia group.

Bottom Line: Our results indicate that pretreatment with resveratrol protects against brain damage, reducing infarct volume, preserving myelination and minimizing the astroglial reactive response.Moreover its neuroprotective effect was found to be long lasting, as behavioral outcomes were significantly improved at adulthood.We speculate that one of the mechanisms for this neuroprotection may be related to the maintenance of the mitochondrial inner membrane integrity and potential, and to the reduction of reactive oxygen species.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology & Histology, School of Medicine & Dentistry, University of the Basque Country (UPV/EHU), Leioa, Bizkaia, Spain.

ABSTRACT
Despite advances in neonatal care, hypoxic-ischemic brain injury is still a serious clinical problem, which is responsible for many cases of perinatal mortality, cerebral palsy, motor impairment and cognitive deficits. Resveratrol, a natural polyphenol with important anti-oxidant and anti-inflammatory properties, is present in grapevines, peanuts and pomegranates. The aim of the present work was to evaluate the possible neuroprotective effect of resveratrol when administered before or immediately after a hypoxic-ischemic brain event in neonatal rats by analyzing brain damage, the mitochondrial status and long-term cognitive impairment. Our results indicate that pretreatment with resveratrol protects against brain damage, reducing infarct volume, preserving myelination and minimizing the astroglial reactive response. Moreover its neuroprotective effect was found to be long lasting, as behavioral outcomes were significantly improved at adulthood. We speculate that one of the mechanisms for this neuroprotection may be related to the maintenance of the mitochondrial inner membrane integrity and potential, and to the reduction of reactive oxygen species. Curiously, none of these protective features was observed when resveratrol was administered immediately after hypoxia-ischemia.

No MeSH data available.


Related in: MedlinePlus