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Similar Squamous Cell Carcinoma Epithelium microRNA Expression in Never Smokers and Ever Smokers.

Kolokythas A, Zhou Y, Schwartz JL, Adami GR - PLoS ONE (2015)

Bottom Line: Examination of The Cancer Genome Atlas (TCGA) project miRNA data on 305 OSCCs and 30 controls revealed 100% of those miRNAs enriched in never smoker OSCCs in this patient group were also enriched in ever smoker OSCCs.Nonsupervised clustering of TCGA OSCCs was suggestive of two or four subgroups of tumors based on miRNA levels with limited evidence for differences in tobacco exposure among the groups.It implies that there may be great similarity in etiology of OSCC in never and ever smokers and that classifying OSCC based on tobacco exposure may not be helpful in the clinic.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, College of Dentistry, University of Illinois at Chicago, 801 South Paulina Street, Chicago, Illinois, 60610, United States of America.

ABSTRACT
The incidence of oral tumors in patients who never used mutagenic agents such as tobacco is increasing. In an effort to better understand these tumors we studied microRNA (miRNA) expression in tumor epithelium of never tobacco users, tumor epithelium of ever tobacco users, and nonpathological control oral epithelium. A comparison of levels among 372 miRNAs in 12 never tobacco users with oral squamous cell carcinoma (OSCC) versus 10 healthy controls was made using the reverse transcription quantitative polymerase chain reaction. A similar analysis was done with 8 ever tobacco users with OSCC. These comparisons revealed miR-10b-5p, miR-196a-5p, and miR-31-5p as enriched in the tumor epithelium in OSCC of both never and ever tobacco users. Examination of The Cancer Genome Atlas (TCGA) project miRNA data on 305 OSCCs and 30 controls revealed 100% of those miRNAs enriched in never smoker OSCCs in this patient group were also enriched in ever smoker OSCCs. Nonsupervised clustering of TCGA OSCCs was suggestive of two or four subgroups of tumors based on miRNA levels with limited evidence for differences in tobacco exposure among the groups. Results from both patient groups together stress the importance of miR196a-5p in OSCC malignancy in both never and ever smokers, and emphasize the overall similarity of miRNA expression in OSCCs in these two risk groups. It implies that there may be great similarity in etiology of OSCC in never and ever smokers and that classifying OSCC based on tobacco exposure may not be helpful in the clinic.

No MeSH data available.


Related in: MedlinePlus

Differentially expressed miRNAs expressed in controls and OSCCs from never smokers and ever smokers.Supervized hierarchical clustering of 50 differentially expressed miRNAs with fold changes above 2, among 9 controls, 12 never smoker OSCCs, and 8 ever smoker OSCCs. Epithelial samples are listed in the columns and miRNAs are in rows. Red indicates higher expression than average and blue indicates lower than average.
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pone.0141695.g001: Differentially expressed miRNAs expressed in controls and OSCCs from never smokers and ever smokers.Supervized hierarchical clustering of 50 differentially expressed miRNAs with fold changes above 2, among 9 controls, 12 never smoker OSCCs, and 8 ever smoker OSCCs. Epithelial samples are listed in the columns and miRNAs are in rows. Red indicates higher expression than average and blue indicates lower than average.

Mentions: Among 372 miRNAs analyzed seven, miR-196a-5p, miR-10b-5p, miR-503-5p, miR-451a, miR-144-3p, miR-187-3p and miR-31-5p, showed increased expression in the OSCC samples of the never smokers, again using the rank product methodology (Table 2c) [29, 30]. Fig 1 shows a heat map with relative expression of the different miRNAs in samples from ever smoker OSCC, never smoker OSCC, and never smoker controls. Again, no miRNAs showed down regulation. This may be because the tumor samples are often a mixture of tumor and normal epithelium while control samples are pure normal epithelium making a loss of expression in tumor hard to detect.


Similar Squamous Cell Carcinoma Epithelium microRNA Expression in Never Smokers and Ever Smokers.

Kolokythas A, Zhou Y, Schwartz JL, Adami GR - PLoS ONE (2015)

Differentially expressed miRNAs expressed in controls and OSCCs from never smokers and ever smokers.Supervized hierarchical clustering of 50 differentially expressed miRNAs with fold changes above 2, among 9 controls, 12 never smoker OSCCs, and 8 ever smoker OSCCs. Epithelial samples are listed in the columns and miRNAs are in rows. Red indicates higher expression than average and blue indicates lower than average.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4636300&req=5

pone.0141695.g001: Differentially expressed miRNAs expressed in controls and OSCCs from never smokers and ever smokers.Supervized hierarchical clustering of 50 differentially expressed miRNAs with fold changes above 2, among 9 controls, 12 never smoker OSCCs, and 8 ever smoker OSCCs. Epithelial samples are listed in the columns and miRNAs are in rows. Red indicates higher expression than average and blue indicates lower than average.
Mentions: Among 372 miRNAs analyzed seven, miR-196a-5p, miR-10b-5p, miR-503-5p, miR-451a, miR-144-3p, miR-187-3p and miR-31-5p, showed increased expression in the OSCC samples of the never smokers, again using the rank product methodology (Table 2c) [29, 30]. Fig 1 shows a heat map with relative expression of the different miRNAs in samples from ever smoker OSCC, never smoker OSCC, and never smoker controls. Again, no miRNAs showed down regulation. This may be because the tumor samples are often a mixture of tumor and normal epithelium while control samples are pure normal epithelium making a loss of expression in tumor hard to detect.

Bottom Line: Examination of The Cancer Genome Atlas (TCGA) project miRNA data on 305 OSCCs and 30 controls revealed 100% of those miRNAs enriched in never smoker OSCCs in this patient group were also enriched in ever smoker OSCCs.Nonsupervised clustering of TCGA OSCCs was suggestive of two or four subgroups of tumors based on miRNA levels with limited evidence for differences in tobacco exposure among the groups.It implies that there may be great similarity in etiology of OSCC in never and ever smokers and that classifying OSCC based on tobacco exposure may not be helpful in the clinic.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, College of Dentistry, University of Illinois at Chicago, 801 South Paulina Street, Chicago, Illinois, 60610, United States of America.

ABSTRACT
The incidence of oral tumors in patients who never used mutagenic agents such as tobacco is increasing. In an effort to better understand these tumors we studied microRNA (miRNA) expression in tumor epithelium of never tobacco users, tumor epithelium of ever tobacco users, and nonpathological control oral epithelium. A comparison of levels among 372 miRNAs in 12 never tobacco users with oral squamous cell carcinoma (OSCC) versus 10 healthy controls was made using the reverse transcription quantitative polymerase chain reaction. A similar analysis was done with 8 ever tobacco users with OSCC. These comparisons revealed miR-10b-5p, miR-196a-5p, and miR-31-5p as enriched in the tumor epithelium in OSCC of both never and ever tobacco users. Examination of The Cancer Genome Atlas (TCGA) project miRNA data on 305 OSCCs and 30 controls revealed 100% of those miRNAs enriched in never smoker OSCCs in this patient group were also enriched in ever smoker OSCCs. Nonsupervised clustering of TCGA OSCCs was suggestive of two or four subgroups of tumors based on miRNA levels with limited evidence for differences in tobacco exposure among the groups. Results from both patient groups together stress the importance of miR196a-5p in OSCC malignancy in both never and ever smokers, and emphasize the overall similarity of miRNA expression in OSCCs in these two risk groups. It implies that there may be great similarity in etiology of OSCC in never and ever smokers and that classifying OSCC based on tobacco exposure may not be helpful in the clinic.

No MeSH data available.


Related in: MedlinePlus