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ATP-Mediated Compositional Change in Peripheral Myelin Membranes: A Comparative Raman Spectroscopy and Time-Of-Flight Secondary Ion Mass Spectrometry Study.

Kutuzov N, Gulin A, Lyaskovskiy V, Nadtochenko V, Maksimov G - PLoS ONE (2015)

Bottom Line: The simultaneous increase in lipid ordering degree, decrease in membrane fluidity and the degree of fatty acid unsaturation were induced by both ATP and adenosine.Mass spectrometry measurements revealed that ATP administration also led to the marked elevation of membrane cholesterol and decrease of phosphotidylcholine amounts.Vesicular lipid transport pathways are considered as possible mechanisms of compositional and structural changes of myelin.

View Article: PubMed Central - PubMed

Affiliation: Biophysics Department, Biological Faculty, Moscow State University, Leninskie gory 1/12, Moscow, Russian Federation, 119991.

ABSTRACT
In the present paper we addressed a mechanism of the myelin reorganization initiated by extracellular ATP and adenosine in sciatic nerves of the frog Rana temporaria. In combination with Raman microspectroscopy, allowing noninvasive live-cell measurements, we employed time-of-flight secondary ion mass spectrometry (TOF-SIMS) to follow the underlying changes in chemical composition of myelin membranes triggered by the purinergic agents. The simultaneous increase in lipid ordering degree, decrease in membrane fluidity and the degree of fatty acid unsaturation were induced by both ATP and adenosine. Mass spectrometry measurements revealed that ATP administration also led to the marked elevation of membrane cholesterol and decrease of phosphotidylcholine amounts. Vesicular lipid transport pathways are considered as possible mechanisms of compositional and structural changes of myelin.

No MeSH data available.


TOF-SIMS spectra of myelin.
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pone.0142084.g003: TOF-SIMS spectra of myelin.

Mentions: We employed the TOF-SIMS to study the effect of ATP and adenosine on the lipid composition of myelin. Nerves incubated with ATP or adenosine were fixed and analyzed. Fig 3 illustrates a representative example for the positive ion mass spectrum of myelin. Due to the specifics of the currently employed method, which are described elsewhere [32, 34], detectable mass range is limited to several thousand of Daltons. Thus lipids, metals and various metabolites can be analyzed using this setup.


ATP-Mediated Compositional Change in Peripheral Myelin Membranes: A Comparative Raman Spectroscopy and Time-Of-Flight Secondary Ion Mass Spectrometry Study.

Kutuzov N, Gulin A, Lyaskovskiy V, Nadtochenko V, Maksimov G - PLoS ONE (2015)

TOF-SIMS spectra of myelin.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4636249&req=5

pone.0142084.g003: TOF-SIMS spectra of myelin.
Mentions: We employed the TOF-SIMS to study the effect of ATP and adenosine on the lipid composition of myelin. Nerves incubated with ATP or adenosine were fixed and analyzed. Fig 3 illustrates a representative example for the positive ion mass spectrum of myelin. Due to the specifics of the currently employed method, which are described elsewhere [32, 34], detectable mass range is limited to several thousand of Daltons. Thus lipids, metals and various metabolites can be analyzed using this setup.

Bottom Line: The simultaneous increase in lipid ordering degree, decrease in membrane fluidity and the degree of fatty acid unsaturation were induced by both ATP and adenosine.Mass spectrometry measurements revealed that ATP administration also led to the marked elevation of membrane cholesterol and decrease of phosphotidylcholine amounts.Vesicular lipid transport pathways are considered as possible mechanisms of compositional and structural changes of myelin.

View Article: PubMed Central - PubMed

Affiliation: Biophysics Department, Biological Faculty, Moscow State University, Leninskie gory 1/12, Moscow, Russian Federation, 119991.

ABSTRACT
In the present paper we addressed a mechanism of the myelin reorganization initiated by extracellular ATP and adenosine in sciatic nerves of the frog Rana temporaria. In combination with Raman microspectroscopy, allowing noninvasive live-cell measurements, we employed time-of-flight secondary ion mass spectrometry (TOF-SIMS) to follow the underlying changes in chemical composition of myelin membranes triggered by the purinergic agents. The simultaneous increase in lipid ordering degree, decrease in membrane fluidity and the degree of fatty acid unsaturation were induced by both ATP and adenosine. Mass spectrometry measurements revealed that ATP administration also led to the marked elevation of membrane cholesterol and decrease of phosphotidylcholine amounts. Vesicular lipid transport pathways are considered as possible mechanisms of compositional and structural changes of myelin.

No MeSH data available.